2224-15-9Relevant academic research and scientific papers
Study of imidazole performance as pseudo-affinity ligand in the purification of IgG from bovine milk
Abbasi, Hassan,Pourrostam-Ravadanaq, Pariya,Safa, Kazem D.
, (2020)
The spherical sepharose CL-6B beads were activated by epichlorohydrin in different epoxy contents (80, 120 and 160 μmolepoxide/mLgel) and, L-histidine and imidazole as pseudo-affinity ligands were covalently immobilized to them. Some linkers with different length, (1,2-ethanediol diglycidyl ether and 1,4-butanediol diglycidyl ether) were synthesized for activation of sepharose and the activated sepharose beads modified with imidazole and the performance of these adsorbents in the purification of immunoglobulin G from bovine milk were evaluated. Among the L-histidine bearing adsorbents, higher adsorption of IgG (0.28 mg/mL) was obtained by adsorbent with the lower concentration of L-histidine. The highest amount of IgG adsorption (0.53 mg/mL) was obtained by imidazole bearing adsorbent with the highest amount of imidazole and Among the adsorbents with synthesized linkers, the adsorbent with 1,2-ethanediol diglycidyl ether showed better performance and was able to purify 0.25 mg/mL IgG with high purity. The synthesized pseudo-affinity adsorbents represented the abbility to purify immunoglobulin G in one-step process with high purity and efficiency.
Quaternary ammonium salt polymer and preparation method and application thereof
-
Paragraph 0061-0062, (2020/05/29)
The invention provides a quaternary ammonium salt polymer and a preparation method and application thereof. The structure of the quaternary ammonium salt polymer contains a hydrophilic group and adjustable hydrophilic/hydrophobic groups such as PPG and PAG. With high surface tension, the quaternary ammonium salt polymer can effectively separate miscellaneous oil wrapping metal cuttings and enablesthe miscellaneous oil to float above a metal processing aqueous solution, so demulsification can be effectively realized, the surface tension of the solution is increased, and the oil floats to a liquid level; and compared with an existing sedimentation additive for a metal working fluid, the quaternary ammonium salt polymer can effectively settle particles in the metal working fluid, and has good application prospects.
Polyoxyethylene ether carboxylic acid dimeric surfactant type drag reducer as well as preparation method and application thereof
-
Paragraph 0087; 0090-0091; 0096; 0099-0100; 0105; 0108-0109, (2019/09/13)
The invention discloses a polyoxyethylene ether carboxylic acid dimeric surfactant type drag reducer as well as a preparation method and application thereof. The polyoxyethylene ether carboxylic aciddimeric surfactant type drag reducer has a structure formula m=2, 3, 4, and n=1, 2, 3. The drag reducer disclosed by the invention is a dimeric surfactant type drag reducer which has a head group of carboxylic acid, and the head group has the advantages of being very good in temperature resistance, salt resistance, environment protection and the like, and is capable of overcoming influence of a high-salt environment upon drag reduction performance, so that the drag reducer disclosed by the invention is high in drag reduction efficiency, good in shearing resistance, long-lasting and stable in drag reduction rate and good in salt resistance; and polyoxyethylene ether carboxylic acid dimeric surfactants of different mass concentrations are dissolved into water, a drag reduction solution whichis high in drag reduction efficiency, good in shearing resistance, long-lasting and stable in drag reduction rate and good in salt resistance can be prepared without any other compounded chemical reagent, solution blending steps are simple, the drag reducer is very convenient to use, and meanwhile, the salt resistance is greatly improved.
Synthesis method of ethylene glycol diglycidyl ether
-
Paragraph 0014; 0015; 0016; 0017; 0018; 0019, (2017/07/21)
The invention relates to a synthesis method of ethylene glycol diglycidyl ether. According to the method, ethylene glycol and epoxy chloropropane are used as raw materials; argon gas is continuously introduced; ring-opening reaction is performed under the combined action of ultrasonic waves and a ternary composite catalyst. A 5A molecular sieve and the ternary composite catalyst are combined, so that the catalyst dosage is greatly reduced; the reaction progress is accelerated; the method is suitable for industrial large-scale production. After the reaction is completed, the temperature is lowered; the mixture and liquid alkaline take ring-closure reaction under the effects of solvent methylbenzene and a 3A molecular sieve supported phase transfer catalyst; the 3A molecular sieve supported phase transfer catalyst has high reaction activity; after the reaction is completed, water washing is very smooth; after methylbenzene and water are removed from a crude product, the ethylene glycol diglycidyl ether with high epoxide value is obtained, wherein the epoxide value can reach 0.78. Compared with the prior art, the synthesis method has the advantages that the selectivity is high; side reactions are few; the reaction effect is good; the yield is high; the product quality is the best; the cost performance is high.
One-pot Three Component Synthesis of ω-(oxathiolan-2-thion-5-yl)-α-oxazolidin-2-ones
Ghrab, Saad,Aroua, Lotfi,Beji, Mohamed
, p. 2397 - 2404 (2017/07/25)
We report a practical and one-pot synthesis of novel series of ω-(oxathiolan-2-thione-5-yl)-α- oxazolidin-2-ones (3a-h). The obtained compounds have been designed, synthesized via reaction of oligoethylene glycols diglycidyl ethers, isocyanate and carbon disulfide in the presence of catalytic amount of lithium bromide. A variety of important oxazolidinone derivatives can be obtained from simple starting materials in good yields and the biological activity of these new products will be investigated in complementary study.
Solvent-free synthesis of dihydroxy dithiacrown ethers
Zoghlami, Houcine,Romdhani-Younes, Moufida,Chaabouni, Mohamed Moncef,Baklouti, Ahmed
scheme or table, p. 881 - 883 (2011/03/20)
The solvent-free reaction of oligoethylene glycol diglycidyl ethers with dimercaptoethane in the presence of benzyltrimethylammonium hydroxide (Triton B) gave, via regiospecific epoxide opening reactions, dihydroxy dithiacrown ethers in excellent yields and in short reaction times.
Diol glycidyl ether-bridged cyclens: Preparation and their applications in gene delivery
Yi, Wen-Jing,Feng, Zhi-Hua,Zhang, Qin-Fang,Zhang, Ji,Li, Ling-Dong,Zhu, Wen,Yu, Xiao-Qi
experimental part, p. 2413 - 2421 (2011/05/14)
Polymeric 1,4,7,10-tetraazacyclododecanes (cyclens) using diol glycidyl ether with different chain length as bridges (5a-e) were designed and synthesized from various diols, 1,7-diprotected cyclen and epichlorohydrin. The molecular weights of the title polymers were measured by GPC with good polydispersity. Agarose gel retardation and fluorescent titration using ethidium bromide showed good DNA-binding ability of 5. They could retard plasmid DNA (pDNA) at an N/P ratio of 4-6 and form polyplexes with sizes around 100-250 nm from an N/P ratio of 10 to 60 and relatively low zeta-potential values (5-22 mV). The cytotoxicity of 5 assayed by MTT is much lower than that of 20 kDa PEI. In vitro transfection against A549 and 293 cells showed that the transfection efficiency (TE) of 5c/DNA polyplexes is close to that of 20 kDa PEI at an N/P ratio of 5. Structure-activity relationship (SAR) of 5 was discussed in their DNA-binding, cytotoxicity, and transfection studies. The TE of 5c/DNA polyplexes could be improved by the introduction of 50 μM of chloroquine, the endosomolytic agents, to pretreated cells. These studies may extend the application areas of macrocyclic polyamines, especially for cyclen.
Dinuclear macrocyclic polyamine zinc(II) complexes linked with flexible spacers: Synthesis, characterization, and DNA cleavage
Peng, Wei,Liu, Pei-Yan,Jiang, Ning,Lin, Hong-Hui,Zhang, Guo-Lin,Liu, Yi,Yu, Xiao-Qi
, p. 374 - 385 (2008/02/01)
Dinuclear macrocyclic polyamine zinc(II) complexes, which have two cyclen groups linked by flexible spacers, have been synthesized as DNA cleavage agents. The structures of these new dinuclear complexes are consistent with the data obtained from elemental analysis, MS and 1H NMR spectroscopy. The catalytic activity of these dinuclear complexes on DNA cleavage was studied. The results showed that the dinuclear zinc(II) complexes can catalyze the cleavage of supercoiled DNA (pUC 19 plasmid DNA) (Form I) under physiological conditions to produce selectively nicked DNA (Form II).
Structure and hardening activity of oxirane gelatin hardeners based on ethylene glycol and ethylenediamine
Serdyuk,Davydova,Chezlov,D'yakonov
, p. 1844 - 1845 (2007/10/03)
Ethylene glycol diglycidyl ether and N,N′-diglycidyl-N,N′-dibutylethylenediamine were prepared, and their cross-linking effect on gelatin was studied, judging from the melting point and mechanical strength of model gelatin layers.
