Separation of heparin on SEPHAROSE CL-4B (cas 61970-08-9) in the presence of high concentrations of ammonium sulphate

Add time:07/12/2019    Source:sciencedirect.com

Heparin was fractionated by chromatography on SEPHAROSE CL-4B (cas 61970-08-9)/3.8–2.0 M ammonium sulphate in 0.01 M hydrochloric acid at 4°C based on a principle different from that of gel filtration —possibly due to multiple interaction mechanisms including those based on hydrophobic bonds. The results of the fractionation were very similar to those of chromatography on Phenyl-Sepharose CL-4B/3.8–2.0 M ammonium sulphate in 0.01 M hydrochloric acid at room temperature. That is, the separation was related to the molecular size distribution, N-acetyl content and anticoagulant activities. As the result of studies on a set of Sepharose 4B gels with different degrees of cross-linking, it has been shown that the introduction of the cross-linked structure, OCH2CH(OH)CH2O, into the gel matrix enhances the interaction between the Sepharose CL-4B gel and heparin, indicating that heparin retention by the highly cross-linked Sepharose 4B gel surpasses that by Phenyl-Sepharose CL-4B.

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