Three independent methods for quantitative determination of octyl covalently coupled to SEPHAROSE CL-4B (cas 61970-08-9)

Add time:07/16/2019    Source:sciencedirect.com

Quantification of the octyl content in Octyl-Sepharose CL-4B was accomplished by three independent methods. Firstly, the 1H NMR spectrum was registered on a deuterium chloride hydrolysed gel. Secondly, gas chromatography was applied to the ether-linked ligands cleaved by boron tribromide. Finally, the gel was combusted to carbon dioxide and elemental carbon analysis was performed. The results from the three methods indicate only random errors at a confidence level of 95%. All developed methods are therefore usable for the determination of the ligand content.

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