Desorption of immunoglobulins from Protein A-SEPHAROSE CL-4B (cas 61970-08-9) under mild conditions

Add time:07/18/2019    Source:sciencedirect.com

Conditions which permit the dissociation of IgG-staphylococcal protein A interactions with resort to low pH buffers or the use of chaotropic ions are discussed in relation to: (a) the mechanism of the binding reaction; (b) the use of immobilised protein A for purification of cells or removal of immune complexes from serum. Glycyl-tyrosine, glycyl-histidine, glycyl-phenylalanine and tryptophan, representing the class of competing ligand desorbents, and ethylene glycol, an agent which disrupts hydrophobic interactions, were found to be the best desorbents.

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