Contractile effects of prostaglandin E2 in rat rectum: Sensitivity to the prostaglandin antagonists diphloretin phosphate and SC 19220 (cas 19395-87-0)

Add time:08/18/2019    Source:sciencedirect.com

Prostaglandin E2 (PGE2) applied cumulatively (1 nM − 1 μM) induced concentration-dependent tonic contractions in the longitudinal muscle of isolated rat rectum. The PGE2 effects were not altered by guanethidine (50 μM), whereas atropine (3 μM), guanethidine plus atropine or tetrodotoxin (0.1 μM) reduced them to an almost equal extent and increased the EC50 values for PGE2. The after-contractions following electrical stimulation were enhanced by PGE2 (10 nM) and inhibited by atropine. Diphloretin phosphate (DPP, 100 μM) shifted the regression lines for PGE2 to the right in both untreated and tetrodotoxin-treated preparations, and thereby increased the EC50 values. Slopes of the concentration-effect lines for PGE2 before and after DPP differed in the presence of tetrodotoxin. The regression line for PGE2 with SC 19220 (100 μM) in tetrodotoxin-treated preparations was shifted to the right in a parallel fashion. It is concluded that PGE2 exerted both a neural (cholinergic) and a smooth muscle effect. There may be a competitive antagonism between SC 19220 and PGE2 but the block by DPP may be nonselective.

We also recommend Trading Suppliers and Manufacturers of SC 19220 (cas 19395-87-0). Pls Click Website Link as below: cas 19395-87-0 suppliers

Prev:Effects of prostaglandin antagonist SC-19220 on body temperature and on hyperthermic responses to prostaglandin E1 and leukocytic pyrogen in the cat
Next:Reversal of the antilipolytic effect of prostaglandin E2 by an oxazepine derivative (SC-19220))