Artificial enzyme-based catalytic sensor for the electrochemical detection of 5-HYDROXYINDOLE-3-ACETIC ACID (cas 1321-73-9) tumor marker in urine

Add time:08/17/2019    Source:sciencedirect.com

A novel approach towards the development of biomimetic electrochemical sensors is presented. It is based on magnetic-core/porous-shell molecularly imprinted composites as recognition phase. The selected substrate is 5-HYDROXYINDOLE-3-ACETIC ACID (cas 1321-73-9) (5-HIAA), an urinary metabolite used as carcinoid tumor maker. Using 5-HIAA as template molecule, hemin, 4-vinylpyridine and ethylenglycoldimethacrylate crosslinker are polymerized onto the magnetic core to obtain an artificial enzyme with peroxidase activity. An aliquot of a suspension of the resulting material is deposited onto a carbon screen printed electrode, and the solid material is fixed with a permanent magnet to prepare the sensor. The product of the catalytic oxidation of 5-HIAA at the sensing phase is monitored by differential pulse voltammetry. This sensor allows the detection of 5-HIAA with good sensitivity (0.72 ± 0.01 nA μM−1) and linear response over a concentration range covering the whole window of normal and pathologic urinary levels of 5-HIAA 1–50 μM (0.2–9.6 ppm) with a detection limit of 1.4 μM (0.27 ppm). Urine samples of healthy individuals, spiked with known concentrations of 5-HIAA, yield good recovery values and reproducibility for normal and pathological concentrations (90–100% recovery values, with RSD below 10%).

We also recommend Trading Suppliers and Manufacturers of 5-HYDROXYINDOLE-3-ACETIC ACID (cas 1321-73-9). Pls Click Website Link as below: cas 1321-73-9 suppliers

Prev:Pharmaceutical NanotechnologyHPMC effect on catalyzed hydrolysis of α-naphthyl acetate in cationic micellar systems
Next:Photo-activated 5-HYDROXYINDOLE-3-ACETIC ACID (cas 1321-73-9) induces apoptosis of prostate and bladder cancer cells)