Short communicationSimultaneous determination of yohimbine hydrochloride, strychnine nitrate and methyltestosterone by ion-pair high-performance liquid chromatography

Add time:09/28/2019    Source:sciencedirect.com

A reversed-phase ion-pair chromatographic method was developed for the simultaneous determination of yohimbine hydrochloride (YOH), strychnine nitrate (SN) and methyltestosterone (MT) in a pharmaceutical preparation (tablet). YOH, SN and MT were extracted and separated within 15 min on an octadecylsilano-silica gel column (15 cm × 4.6 mm I.D., 5 μm particle size). The mobile phase was a mixture of 5 · 10−3M sodium octanesulphonate solution (pH 5.5) and methanol (40:60, v/v). The ultraviolet absorption detector was set at 254 nm, and noscapine was used as an internal standard. The recoveries of YOH, SN and MT from a known sample were 101.9 and 101.8 and 99.4%, respectively. By this method, these components in commercial tablets could be simultaneously determined without any interference. The method is sufficiently sensitive, reproducible and accurate.

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