Reference

Comparison of the kinetics and utilization of D(-)- and L(+)-sodium lactate in normal man

Comparison of the kinetics and utilization of D(-)- and L(+)-sodium lactate in normal man. Connor, H.; Woods, H. F.; Ledingham, J. G. G. (Univ. Dep. Ther., R. Hallamshire Hosp., Sheffield, UK). Ann. Nutr. Metab., 27(6), 481-7 (English) 1983. CODEN: ANUMDS. 72-17-3 and 312-85-6 are cas registry numbers of chemicals which are used as reagents here. ISSN: 0250-6807. DOCUMENT TYPE: Journal CA Section: 18 (Animal Nutrition) After infusion of Na D(-)L(+)-lactate [312-85-6] in healthy humans the clearance of the D(-)-isomer from blood was 70% of that of the L(+)-isomer. Utilization of L(+)-lactate [79-33-4] may have been inhibited by the presence of the D(-)-isomer. The changes in blood pyruvate concn. and ketone body ratio were compatible with mitochondrial oxidn. of D(-)-lactate [10326-41-7] to pyruvate. After infusion of a D(-)L(+)-lactate racemic mixt., the renal excretion of the D(-)-isomer was much greater than that of the L(+)-isomer, although peak blood concns. of the L(+)-isomer were higher than those of the D(-)-isomer. After infusion of Na L(+)-lactate [867-56-1], the renal excretion of D(-)-lactate increased 7-fold, although no D(-)-lactate could be detected in the blood or in the Na L(+)-lactate infused. .

Production of optivally active lactic acid

Production of optivally active lactic acid. (Unitika Ltd., Japan). Jpn. Kokai Tokkyo Koho JP 59031690 A2 20 Feb 1984 Showa, 4 pp. (Japanese). (Japan). CODEN: JKXXAF. CLASS: IC: C12P007-56. APPLICATION: JP 82-140294 11 Aug 1982. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) Racemic 2-halopropionic acid is treated with a dehalogenating enzyme that is specific for either optical isomer and then with another enzyme that reacts with both optical isomers to yield either D- [10326-41-7] or L-lactic acid [79-33-4]. Thus, 100 mL of a soln. contg. 0.1M DL-2-chloropropionic acid [62138-52-7] and 0.05 g NaN3 was adjusted to pH 9-9.5 with NaOH, mixed with 10 units of L-2-haloacid dehalogenase [37289-39-7], and incubated at 30° at a pH of 10-10.5. At 12 and 24 h, 10 units of L-2-haloacid dehalogenase were added to the mixt. HCl was added to a pH of 2 at 36 h. The mixt. was concd. and worked up by column chromatog. on Dowex 1 to yield 4.05 g D-lactic acid. The column was eluted with N HCOOH. The eluant was evapd. to dryness. The residue was dissolved in 100 mL H2O contg. 0.05 g NaN3 and adjusted to pH 9.5 with NaOH. About 10 units of DL-2-haloacid dehalogenase [89511-96-6] were added. The mixt. was incubated at 30°. At 12 and 24 h, 10 units of DL-2-haloacid dehalogenase were added. The mixt. was adjusted to pH 2 with HCl at 36 h, concd., and crystd. with EtOH to obtain 3.65 g L-lactic acid.Except for chemicals metioned above, 10326-41-7 and 37289-39-7 are also used. .