Xi,
XnRelevant articles and documents
All total 12 Articles be found
All total 12 Articles be foundA large scale synthesis of mono- and di-urethane derivatives of lysine
Wiejak, Stanislaw,Masiukiewicz, Elzbieta,Rzeszotarska, Barbara
, p. 1489 - 1490 (1999)
In the search for a practical route to lysine diurethane derivatives useful for peptide synthesis, we elaborated the synthesis of N(ε)-tert- butoxycarbonyl-L-lysine copper(II) complex (1). This served as substrate for obtaining N(ε)-tert-butoxycarbonyl-L-lysine (2), N(α)-benzyloxycarbonyl- N(ε)-tert-butoxycarbonyl-L-lysine dicyclohexylamine salt (3) and N(α)-(9- fluorenyl)methoxycarbonyl-N(ε)-tert-butoxycarbonyl-L-lysine (4).
A Universal Labeling Strategy for Nucleic Acids in Expansion Microscopy
Hofkens, Johan,Leen, Volker,Rohand, Taoufik,Vandereyken, Katy,Vanheusden, Marisa,Voet, Thierry,Wen, Gang
supporting information, p. 13782 - 13789 (2021/09/11)
Expansion microscopy (ExM) enables the nanoscale imaging of ribonucleic acids (RNAs) on a conventional fluorescence microscope, providing information on the intricate patterns of gene expression at (sub)cellular resolution and within spatial context. To extend the use of such strategies, we examined a series of multivalent reagents that allow the labeling and grafting of deoxyribonucleic acid (DNA) oligonucleotide probes in a unified approach. We show that the reagents are directly compatible with third-generation in situ hybridization chain reaction RNA FISH (fluorescence in situ hybridization) techniques while displaying complete retention of the targeted transcripts. Furthermore, we validate and demonstrate that our labeling method is compatible with multicolor staining. Through oligonucleotide-conjugated antibodies, we demonstrate excellent performance in ×4 ExM and ×10 ExM, achieving a resolution of ～50 nm in ×10 ExM for both pre- and postexpansion labeling strategies. Our results indicate that our multivalent molecules enable the rapid functionalization of DNA oligonucleotides for ExM.
Method for synthesizing N-(carbobenzoxy) succinimide by one-pot two-phase method
-
Paragraph 0024-0029, (2020/02/14)
The invention relates to the technical field of organic chemistry, in particularly to a method for synthesizing N-(carbobenzoxy) succinimide by one-pot two-phase method. The method comprises the following steps: adding purified water and hydroxylamine sulfate into a reaction container, dropwise adding liquid caustic soda while stirring, adding butanedioic anhydride in batches after dropwise addingis finished, and carrying out high-temperature vacuum dehydration under acid catalysis until no water is extracted so as to prepare an N-hydroxysuccinimide solution; adding an alkali into the N-hydroxysuccinimide solution to adjust the pH value of the reaction solution, controlling the temperature at 0-60 DEG C, and dropwise adding benzyl chloroformate; after dropwise adding, separating out an organic layer, concentrating to be dry, and recrystallizing to obtain an N-(carbobenzoxy) succinimide finished product. According to the method provided by the invention, separation and purifying stepsafter synthesis of the N-hydroxysuccinimide are avoided, and the N-(carbobenzoxy) succinimide is synthesized by directly adopting a two-phase method in a one-pot manner, so that the process cost is effectively reduced, the operation steps are reduced, and large-scale industrial production is facilitated.
Scalable synthesis of Nα-benzyloxycarbonyl-L-ornithine and of Nα-(9-fluorenylmethoxy)carbonyl-L-ornithine
Masiukiewicz, Elzbieta,Wiejak, Stanislaw,Rzeszotarska, Barbara
, p. 531 - 537 (2007/10/03)
-
