Bioorganic and Medicinal Chemistry Letters p. 1057 - 1060 (1998)
Update date:2022-08-02
Topics:
Hendrickson, H. Stewart
Hendrickson, Elizabeth K.
1,2-Dimyristoyloxypropane-3-thiophosphate(rac-1-myo-inositol-4- phosphate), a thiophosphate analog of dimyristoyl phosphatidylinositol-4- phosphate was synthesized as a substrate for mammalian phosphoinositide- specific phospholipase C. Its activity with A(1-132)-PI-PLC-δ1 (a deletion mutant with the N-terminal pleckstrin homology domain removed) was studied in sonicated dispersions, with and without added Triton X-100. It had an initial activity of about 30 μmol min-1 mg-1, which rapidly decreased due to substrate depletion in the vesicle or micelle. The slower rate of hydrolysis appeared limited by enzyme hopping or exchange of substrate between vesicles or micelles, which was more rapid in the presence of detergent.
View MoreGuangzhou Chemical Reagent Factory
Contact:+86-20-8435 9820 or 8435 7345
Address:Southern Guangzhou, Guangdong, China
Dongying J&M Chemical Co., Ltd,
Contact:546-8551108
Address:Room 1219, Zisheng Mansion, Zibo Road, Dongying, Shandong, China
Contact:86-898-65311214
Address:Room 102, BLDG. 68 Jiangnan City, No. 66 Heping Road,Haikou, Hainan, China
website:http://www.shochem.com
Contact:021-50800795
Address:No.1043, Halei Rd, Zhangjiang Hi-Tech Park, Shanghai, Postcode 201203, China
Jingzhou TianHe Sci&Tech Chemical Co., Ltd.
Contact:86-716-8331612
Address:Jiangjin Road, #18, High-grade technology industries development district, Jingzhou city, Hubei province
Doi:10.1016/j.bmcl.2011.04.143
(2011)Doi:10.1055/s-0028-1083353
(2009)Doi:10.1002/adsc.200800316
(2008)Doi:10.1021/ja00219a085
(1988)Doi:10.1021/ol900364y
(2009)Doi:10.1007/s00706-008-0917-1
(2008)