8.0 Hz, H-5), 5.47 (dd, 1H, J1¢¢,2¢¢ 1.6, J1¢¢,P 8.0 Hz, H-1¢¢), 4.19–
4.22 (m, 2H, H-2¢, H-3¢), 4.19–4.22 (m, 2H, H-2¢, H-3¢), 4.11–
4.14 (m, 1H, H-4¢), 4.02–4.09 (m, 2H, H-5¢a, H-5¢b), 3.96–4.00
(m, 1H, H-5¢¢), 3.78–3.85 (m, 3H, H-2¢¢, H-3¢¢, H-4¢¢), 3.68 (dd,
1H, J5¢¢,6¢¢a, 7.2, J6¢¢a,6¢¢b 12.0 Hz, H-6¢¢a), 3.61 (dd, 1H, J5¢¢,6¢¢b, J6¢¢a,6¢¢b
4.8 Hz, H-6¢¢b); 31P NMR (D2O): d -7.84 (d, 1P, J 20.4 Hz, Pb),
-10.13 (d, 1P, J 20.4 Hz, Pa); LRESIMS 565 ([M - H]-, 1%),
75 (100%). HRESIMS found m/z 565.0473 [M - H]-. Calcd for
C15H23N2O17P2 565.0478. The 1H NMR spectroscopic data for this
compound are consistent with those reported previously.50
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Acknowledgements
We thank Dr Monica Tello for assistance with protein expression,
Dr Martin Rejzek for advice and assistance with HPLC analyses
and Professor Andrea Vasella for helpful suggestions. This work
was supported by the UK Engineering and Physical Sciences
Research Council (Grant ref: GR/S82053/02), the Biotechnology
and Biological Sciences Research Council (core support grant
to the John Innes Centre), and through a Wellcome Trust
International Research Collaboration grant.
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