Journal of the American Chemical Society p. 13371 - 13380 (2010)
Update date:2022-09-26
Topics:
Chang, Shih-Huang
Han, Jeng-Liang
Tseng, Susan Y.
Lee, Hsin-Yu
Lin, Chin-Wei
Lin, Yu-Chen
Jeng, Wen-Yih
Wang, Andrew H.-J.
Wu, Chung-Yi
Wong, Chi-Huey
A new type of glycan array covalently or noncovalently attached to aluminum oxide-coated glass (ACG) slides has been developed for studies of enzymatic reactions and protein binding. To prepare the noncovalent array, glycans with a polyfluorinated hydrocarbon (-C8F17) tail are spotted robotically onto the ACG slide surface containing a layer of polyfluorinated hydrocarbon terminated with phosphonate. After incubation and washing, the noncovalent array can be characterized by MS-TOF via ionization/desorption at a low laser energy without addition of matrix. A representive cellotetraose array was developed to study the activity and specificity of different cellulases and to differentiate the exo-and endoglucanase activities. To prepare the covalent array, glycans with a phosphonic acid tail were synthesized and spotted robotically onto the ACG slide surface. After incubation, the slides can be used directly for quantitative protein binding analysis. Compared to the preparation of glycan arrays on glass slides and other surfaces, this method of arraying using phosphonic acid reacting with ACG is more direct, convenient, and effective and represents a new platform for the high-throughput analysis of protein-glycan interactions.
View MoreShandong Xingshun New Material Co., Ltd.
website:http://www.sd-xingshun.com
Contact:+86-519-86461196/+86-519-86464994
Address:Middle of Luhua East Road, Dingtao District
Guangzhou Chemical Reagent Factory
Contact:+86-20-8435 9820 or 8435 7345
Address:Southern Guangzhou, Guangdong, China
Hangzhou Zyter Biological & Chemical Technology Co., Ltd.
website:http://www.zyterpharm.com
Contact:+86-18858184290
Address:West Wenyi Road, Cangqian, Yuhang
Hangzhou Think Chemical Co. Ltd
website:http://www.thinkchem.com/
Contact:86-571-89986307/81956191/81956084/81956192
Address:Room 501-502, Tower E, Yuanjian Building, Yuanyang International Center, Hangzhou-310011, China
Contact:+86-28-88523492
Address:714rooms of Time Square, Pujiang County
Doi:10.1021/jo00273a012
(1989)Doi:10.1007/s00726-010-0762-5
(2011)Doi:10.1002/jccs.200900150
(2009)Doi:10.1039/b918729d
(2009)Doi:10.1016/j.tet.2009.10.104
(2010)Doi:10.1002/ejoc.200900917
(2009)