ISOLATION AND CHARACTERIZATION OF BRANCHED CYCLODEXTRINS

Article abstract of DOI:10.1016/S0008-6215(00)90195-2

Three branched cyclodextrins (CDs) were isolated by high-performance liquid chromatography (l.c.) from the mother liquors of a large-scale preparation of the unbranched CDs with Bacillus ohbensis cyclomaltodextrin glucanotransferase.Evidence from chromatographic behavior on three l.c. coloumns of different separation modes, fragmentation analysis, ¹³C-n.m.r. spectroscopy, methylation analysis, and fast-atom bombardment-mass spectroscopy (f.a.b.-m.s.) indicated that these compounds were 6-O-α-D-glucopyranosylcyclomaltohexaose (1), 6-O-α-D-glucopyranosylcyclomaltoheptaose (2), and 6,6'''-di-O-α-D-glucopyranosylcyclomaltoheptaose (3).