Straightforward Preparation and Use in Oligodeoxynucleotide Synthesis of 5'-O-(4,4'-Dimethoxytrityl)-4-Thiothymidine

Article abstract of DOI:10.1016/S0040-4039(00)74217-1

The title compound was prepared in four steps and 62percent yield from thymidine.After incorporation into synthetic oligodeoxynucleotides, the S-(2-cyanoethyl) group was removed by treatment with DBU in CH3CN to give products containing 4-thiothymidine residues.