Enzymatic Synthesis of the Delicious Peptide Fragments in Eutectic Mixtures

Article abstract of DOI:10.1021/jf00057a040

The feasibility of flavor peptide production has been investigated using proteases in liquid/semiliquid eutectic mixtures comprised of the substrates together with small amounts of water and alcohols (19-24percent w/w).The three functional fragments of the so-called "Delicious Octapeptide", L-Lys-Gly, L-Asp-Glu-Glu, and L-Ser-Leu-Ala, were obtained in good yields without any requirement for the regioselective side-chain protection of multifunctional amino acids.The peptides were synthesized on a preparative scale in overall yields of 29-77percent and were fully characterized.Keywords: Delicious Peptide; food peptides; flavor peptides; enzymatic peptide synthesis; eutectic mixtures; proteases; adjuvants