Rigid phencyclidine analogues. Binding to the phencyclidine and σ1 receptors

Article abstract of DOI:10.1021/jm970059p

Three phencyclidine (PCP) analogues possessing a highly rigid carbocyclic structure and an attached piperidine ring which is free to rotate were synthesized. Each analogue has a specific fixed orientation of the ammonium center of the piperidinium ring to the centrum of the phenyl ring. The binding affinities of the rigid analogues 1-piperidino-7,8- benzobicyclo[4.2.0]octene (14), 1-piperidinobenzobicyclo[2.2.1]heptene (16), and 1-piperidinobenzobicyclo[2.2.2]octene (13) for the PCP receptor ([³H]TCP) and σ-receptor (NANM) were determined. The three analogues show low to no affinity for the PCP receptor but good affinity for the σ-receptor and can be considered σ-receptor selective ligands with PCP/σ ratios of 13, 293, and 368, respectively. The binding affinities for the σ-receptor are rationalized in terms of a model for the σ-pharmacophore.

Full text of DOI:10.1021/jm970059p

468
J . Med. Chem. 1998, 41, 468-477
Rigid P h en cyclid in e An a logu es. Bin d in g to th e P h en cyclid in e a n d σ1 Recep tor s
Robert M. Moriarty,*,† Livia A. Enache,† Lei Zhao,† Richard Gilardi,‡ Mariena V. Mattson,§ and Om Prakash†,|,
Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois 60607-7061, Laboratory for the Structure of
Matter, Naval Research Laboratory, Washington, D.C. 20375-5341, and Laboratory of Medicinal Chemistry NIDDK, NIH,
Bethesda, Maryland 20892
Received J anuary 31, 1997
Three phencyclidine (PCP) analogues possessing a highly rigid carbocyclic structure and an
attached piperidine ring which is free to rotate were synthesized. Each analogue has a specific
fixed orientation of the ammonium center of the piperidinium ring to the centrum of the phenyl
ring. The binding affinities of the rigid analogues 1-piperidino-7,8-benzobicyclo[4.2.0]octene
(14), 1-piperidinobenzobicyclo[2.2.1]heptene (16), and 1-piperidinobenzobicyclo[2.2.2]octene (13)
for the PCP receptor ([3H]TCP) and σ-receptor (NANM) were determined. The three analogues
show low to no affinity for the PCP receptor but good affinity for the σ-receptor and can be
considered σ-receptor selective ligands with PCP/σ ratios of 13, 293, and 368, respectively.
The binding affinities for the σ-receptor are rationalized in terms of a model for the
σ-pharmacophore.
In tr od u ction
PCP (phencyclidine, 1-(1-phenylcyclohexyl)piperidine)
(1) was originally introduced as a general anesthetic
agent,1-3 but it was subsequently withdrawn from use
in humans because of severe psychomimetic side
effects.4-9 The focus of research on PCP has shifted
from its use as an anesthetic toward potential applica-
tions as a neuropharmaceutical.10,11 This effort has
been spurred by the observation of anticonvulsant12-14
and neuroprotective activity in rodents.15 The search
for noncompetitive NMDA antagonists has been sub-
stantially assisted by the development of a reliable
binding assay,16a and to date a large number of PCP
analogues have been synthesized and assayed as sub-
The NMDA subclass of glutamate receptors are
strates for the PCP binding site.16b-j Among the non-
composed of an ion channel which possesses multiple
competitive NMDA antagonists, 1-[(2-thienyl)cyclohexyl]-
sites for agonist and antagonist binding. L-Glutamate
is a fast excitatory neurotransmitter which acts upon
piperidine (TCP),17 (+)-N-allyl-N-normetazocine ((+)-
NANM, (+)-SKF 10,047),18 and MK-80119-21 are
ligand-gated ion channel receptors and is the endog-
enous ligand for the NMDA receptor.30-34 NMDA (N-
methyl-D-aspartate) is a synthetic compound. Figure
1 represents a schematic representation of the NMDA
intracellular ion channel receptor.35
prototypical.
Neuroprotection can be understood in terms of nerve
cell death resulting from excessive stimulation caused
by L-glutamate at excitatory synapses within the
CNS.22-29 Ischemia or reduced supply of oxygen (anoxia/
hypoxia/ischemia) to the brain caused by birth asphyxia,
traumatic head injury, stroke, or hypoglycemia results
in unregulated calcium ion influx through a ligand-
gated ion channel at a receptor which has N-methyl-D-
aspartic acid (NMDA) as a specific ligand. This patho-
physiology of neuron cell death has been termed
excitotoxicity, and the calcium influx may result in
osmolytic swelling, free radical production, and super-
oxide production, all of which contribute to neuron
destruction.
In Figure 1, L-glutamate is the endogenous ion
channel agonist; glycine is a coagonist.36 Within the ion
channel, Mg2+ performs a regulatory function.37 At rest,
Mg2+ blocks the ion channel, and a negative intracel-
lular membrane potential exists. The Mg2+ block is
voltage dependent and is removed if the cell is partially
depolarized.38 Also within the ion channel, as shown
in Figure 1, are the receptor sites for MK-801 and
PCP.39-42 This is the link to neuroprotection for both
of these agents because they can counter the effects of
excess L-glutamate excitotoxicity by blockade of the ion
channel to ion influx.
† University of Illinois at Chicago.
The neuroprotective activity of both MK-80123,24,43 and
PCP29 has been amply demonstrated.
Several PCP-like molecules cross react with the PCP
receptor, the σ receptor, and the dopamine-D2 recep-
tors.44 The high-affinity [3H]-N-allyl-N-normetazocine
((+)-SKF-10,047) binding site originally identified by
‡ Naval Research Laboratory.
§ NIH.
| On sabbatical leave from Kurukshetra University, Kurukshetra
132119, India.
A report of this work was presented at the Fifty-Third Annual
Scientific Meeting of the Committee on Problems of Drug Dependence,
Inc., Palm Beach, FL, J une 16-20, 1991.
S0022-2623(97)00059-9 CCC: $15.00 © 1998 American Chemical Society
Published on Web 01/10/1998

Rigid Phencyclidine Analogues
J ournal of Medicinal Chemistry, 1998, Vol. 41, No. 4 469
Sch em e 1
F igu r e 1. Schematic NMDA receptor calcium ion channel
complex.
Martin et al.45 in 1976 as an opioid receptor type is now
called the σ site.46 PCP and (+)-SKF-10,047 bind to
both PCP and σ receptors.47 A distinction between the
sites based upon ligand selectivity is revealed by the
fact that [3H]-(+)-SKF-10,047 is displaced by neurolep-
tics such as haloperidol and perphenazine, while these
compounds show no ability to displace PCP-like com-
pounds.48,49
conformational analysis is that the rotational angle of
Φ ) 90° places the ammonium center near the centrum
of the phenyl ring. The angle of Φ ) 0° has the
ammonium center in the plane of the phenyl ring. It
has been concluded on the basis of 1H NMR, 13C NMR,
MM-2 calculations, and X-ray structure that the Φ )
90° angle65 is the stable form of the active pharmacoph-
ore. This proposal has been tested experimentally in
the cases of the rigid analogue aminohexahydrofluorene
2 which conforms to Φ ) ca. 90°.66
The physical nature of the σ receptor has not yet been
fully defined, even though several classes of selective
ligands have been identified. σ Receptors are widely
found, occurring both in the nervous system and pe-
ripheral tissue such as liver, kidney, and intestine.50 The
range of σ receptor substrates is likewise broad, includ-
ing progesterone51 and inhibitors of cytochrome P-450.52,53
Numerous physiological and pharmacological roles
have been suggested for σ receptors, although to date
no drug has been developed based on the σ receptor.54,55
The absence of an identified endogenous ligand does not
add to the physical characterization of this receptor.56
The σ receptor is implicated in antiischemic and
neuroprotective action.57-61 Subtypes of σ recognition
sites have been proposed based upon selectivity of
binding of various ligands. The σ1 site exhibits high
affinity for (+)-benzomorphans such as (+)-pentazocine
and (+)-N-allyl-N-normetazocine (SKF-10,047), and (-)-
benzomorphans are σ2 site ligands.62 The pharmacoph-
ore for σ binding is multivariant. The distinction
between the two sites is illustrated by the observation
that N-phenylalkyl substitution of N-normetazocine
significantly enhanced affinity for the σ site labeled with
[3H]-(+)-3-PPP while affinity for PCP sites was de-
creased.63 (+)-Pentazocine [(+)-N-(3,3-dimethylallyl)-N-
normetazocine] bound with higher affinity than (+)-N-
allyl-N-normetazocine to σ receptors.63,64 The N-
phenylpropyl-, -butyl-, and -pentyl-N-normetazocine
derivatives also showed affinity for σ sites.
Also, the four enantiomers corresponding to cis- and
trans-fused 8a-phenyldecahydroquinolines, 3 and 4,
were assayed for affinity for the PCP receptor with the
conclusion that the anti N-H/C-phenyl arrangement is
the preferred orientation for optimal binding.67
The structural requirements for σ binding have been
indicated by the affinities of a series of molecules which
possess the phenylpiperidino group. Thus, 2-(4-phe-
nylpiperidino)ethyl 1-(4-nitrophenyl)cyclopentanecar-
boxylate hydrochloride (5‚HCl) showed an affinity for
the (+)-pentazocine binding site with a Ki of 50 pM, and
this compound was inactive at the PCP, NMDA, and
opioid receptors.68
Some generalizations about structure-activity at the
PCP and σ receptors can be made. PCP is a relatively
flexible molecule which can undergo conformational ring
inversion of the cyclohexyl and piperidinyl rings as well
as rotation of the phenyl group about the carbon-carbon
single bond (Scheme 1).
Under physiological conditions the protonated form
can undergo a chair-chair conformational change for
both the cyclohexyl and the piperidinyl rings, while the
phenyl group can adopt two limiting rotational positions
(Φ ) ca. 0° or ca. 90°). The essential feature of this
Other potent σ ligands were obtained by interposing
methylene groups between the phenylcycloalkyl group
and the nitrogen atom in the PCP framework as in 6.68

470 J ournal of Medicinal Chemistry, 1998, Vol. 41, No. 4
Moriarty et al.
Activity at the σ site increases and affinity at the PCP
receptor decreases by increasing the distance between
the nitrogen atom and the phenyl ring, assuming the
phenylcycloalkyl group occupies the first lipophilic site
and not vice versa. The above results as well as
molecular modeling studies support this view. Accord-
ing to this, “stretched” molecules rather than “globular”
ones are better ligands for the σ site.69 However, there
are some potent σ ligands which seem to be more
“globular” than “stretched”, for example, benz[f]iso-
quinoline derivatives70 and spiropiperidine analogues.71
An extremely potent σ ligand is 7, and several
variations upon this structure, such as 8, exhibit Ki of
0.34 and 0.17 nM for displacement of 1-n-propyl-3-(3-
hydroxyphenyl)piperidine.72
F igu r e 2. X-ray structure of compound 13.
Sch em e 2
(+)3-PPP (11) (IC50 13 nM in σ binding) and PRE-
084 (12) (IC50 44 nM in σ binding and greater than
100 000 nM for PCP) are likewise selective σ ligands.69
Again, these molecules follow the stretched rather than
globular shape.
In the present study, rigid analogues of PCP were
designed and synthesized in order to determine selectiv-
ity between the PCP and σ sites; the strategy of fixing
the orientation of the ammonium center of PCP with
respect to the centrum of the phenyl ring via carbon-
carbon connection was pursued.
The σ binding site is thought to be composed of a
primary lipophilic site, a nitrogen binding site, and a
second lipophilic site. Caramiphen (9) binds with high
affinity (26 nM) to the [3H]-(+)-pentazocine site, and
carbetapentane (10) binds with an IC50 value of 32 nM.
Neither inhibits PCP binding.69
This was accomplished synthetically by tying down
the rotating axial phenyl group into three limiting
values for Φ. In the first case, Φ ≈ 0°, a C-C bond
between the ortho position of the axial phenyl ring of
PCP and C4 of the cyclohexyl ring of PCP (Scheme 2,
bond a) yields the achiral 1-piperidinobenzobicyclo[2.2.2]-
octene (13).
Because of the conformational rigidity of the bicyclo-
[2.2.2]octene ring, the nitrogen atom of the piperidino
ring occupies a position unambiguously in the plane of
the aromatic ring. The X-ray structure shown in Figure
2 shows clearly this structural feature.
The second limiting structure for PCP is one in which
the phenyl ring is twisted toward the nitrogen atom of
the piperidine ring and this geometry is achieved by
incorporation of a C-C bond between the ortho position
of the axial phenyl ring of PCP and the C2 position of
the cyclohexyl ring (Scheme 3, bond b), to yield the
chiral 1-piperidino-7,8-benzobicyclo[4.2.0]octene (14)
(Φ˜60°).
Because of the conformational inflexibility of the
cyclobutene ring, the nitrogen atom of the piperidine is
above the plane of the benzenoid ring. This structure
is similar to the aminohexahydrofluorene (2); however,

Rigid Phencyclidine Analogues
J ournal of Medicinal Chemistry, 1998, Vol. 41, No. 4 471
Sch em e 3
Sch em e 5a
Sch em e 4
the annulated four-membered ring in 14 relative to the
five-membered ring in 2 has the effect of moving the
nitrogen atom closer to the centrum of the aromatic ring.
An intermediary fixed orientation rigid analogue
(Scheme 4, bond c) is also conceivable, and this is
achieved in 15 (Φ ≈ 30°). Compound 15 was not
synthesized, but in fact the lower homologous
benzobicyclo[2.2.1]norbornyl derivative 16 (Φ ≈ 18°)
was. The reason for this choice is based on synthetic
reasons. The sequence 26 f 27 f 28 f 29 (Scheme 7)
benefits from the presence of a plane of symmetry in
4-phenylcyclohexanone 26, which means 27, 28, and 29
are formed as unique regioisomers. An analogous
sequence starting from 4-phenylcycloheptanone is com-
plicated by the absence of a plane of symmetry perpen-
dicular to both rings. Therefore, the Favorskii reaction
yields two isomers. Subsequent reactions analogous to
27 f 28 f 29 would yield two isomeric phenylcyclo-
hexanecarboxylic acids, only one of which could yield
the desired intramolecular Friedel-Crafts product analo-
gous to 28b f 29. Because of uncertainties in the
Favorskii ring contraction as well as the feasibility of
the subsequent reactions, we elected to synthesize the
compound for which closely analogous procedures ex-
isted.
a
(i) NHEt2, KOH, C6H6; (ii) CH3OOCCHdCH2; (iii) HCl, C6H6,
160-170 °C; (iv) 1,2-C6H4NH2(COOH), isoamyl nitrite, 70 °C; (v)
(a) Pd-C, H2, 30 psi, (b) KOH, H2O, (c) PCl5, CH2Cl2, -78 °C, (d)
NH3, -78 °C to rt; (vi) C6H5I(OH)OTs, CH3CN reflux; (vii)
Br(CH2)5Br, K2CO3, DMF reflux.
Sch em e 6
(24) and benzyne as shown in Scheme 6. The enamine
24 (prepared by reaction of piperidine with cyclohex-
anone) was added to fluorobenzene 25 in ether, and
addition of butyllithium yielded the [2 + 2] cycloaddition
product via the in situ generated benzyne.
Ch em istr y
The synthesis of 1-piperidinobenzobicyclo[2.2.2]octene
(13) proceeded from the Diels-Alder reaction between
methyl 1,3-cyclohexadiene-1-carboxylate with benzyne
via the sequences shown in Scheme 5.
The rigid analogue of intermediary rotational angle
was synthesized as shown in Scheme 7. The starting
point for the synthesis of 1-piperidinobenzobicyclo[2.2.1]-
heptene (16) was the hypervalent iodine Favorskii ring
contraction upon 4-phenylcyclohexanone (26). The trans-
formed methyl 3-phenylcyclopentane-1-carboxylate (27)
was carbomethoxylated to yield dimethyl 3-phenylcy-
clopentane-1,1-dicarboxylate (28a ). Conversion to the
free diacid 28b followed by an intramolecular Friedel-
Crafts reaction according to the procedure of Eaton et
al.76 yielded keto acid 29. Hypervalent iodine iodinative
decarboxylation77 yielded the bridgehead iodo ketone 30.
Favorskii ring contraction yielded benzobicyclo[2.2.1]-
heptene-1-carboxylic acid 31a . The hypervalent iodine
Hofmann rearrangement74 upon the amide derived from
the carboxylic acid 31b yielded the bridgehead amine
32 which was converted to 1-piperidinobenzobicyclo-
[2.2.1]heptene 16.78
The requisite diene 20 was prepared using a proce-
dure of Grob et al.73 via enamine formation upon
crotonaldehyde (17) to yield 1-(N,N-diethylamino)-1,3-
butadiene (18) and subsequent cycloaddition with meth-
yl acrylate followed by loss of Et2NH (19 f 20).79 The
Diels-Alder reaction between diene 20 and in situ
formed benzyne afforded the adduct 21. Catalytic
hydrogenation upon the Diels-Alder adduct 21 yielded
methyl 2,3-benzobicyclo[2.2.2]oct-2-ene-1-carboxylate 22a
which was hydrolyzed to yield 2,3-benzobicyclo[2.2.2]-
oct-2-ene-1-carboxylic acid (22b), which in turn was
converted to the amide 22c via the acid chloride derived
from the acid 22b and subsequently to the amine 23
using a hypervalent iodine variation of the Hofmann
amide rearrangement.74 The resulting bridgehead amine
23 was converted to 13 using the method of Gabrielevitz
et al.75
The X-ray structure of 13 is shown in Figure 2. The
structures of 14 and 16 are established by 1H NMR, 13
NMR, and high-resolution mass spectrometry.
C
Analogue 14 was likewise produced by a [2 + 2]
cycloaddition reaction between 1-piperidinocyclohexene

472 J ournal of Medicinal Chemistry, 1998, Vol. 41, No. 4
Moriarty et al.
Sch em e 7a
exists in two enantiomeric forms; each individually is
expected to have a different binding affinity, as the
presence of the relatively bulky piperidino group versus
the N-ethyl group accounts for part of the difference.
Inspection of the binding affinities for the three rigid
analogues 13, 14, and 16 reveals that 13 and 16 are
not ligands for the PCP receptor, and 14 shows a
potency relative to PCP itself of about 2%. The fact that
14 corresponds to Φ ) ca. 60°, which is closer to the
optimal orientation, while 13 and 16 have Φ ) ca. 0°
and Φ ) ca. 20-30°, respectively, which are closer to
the less preferred geometry, agrees with the relative
order of the binding: 117.5, 107, 4.27 µM. Comparison
of (R)- and (S)-33 with 14 is compromised by the fact
that [NH2Et]+ is matched with the piperidine group.
Recently, Kozikowski and co-workers67 have pointed
out the importance of an anti relationship between the
N-H bond of the protonated piperidino ring and the
C-C bond joining the phenyl ring to the quaternary
carbon atom in PCP as well as in the case of cis- and
trans-fused 8a-phenyldecahydroquinolines for effective
binding at the PCP receptor. For analogues 13, 14, and
16, the energy difference between the anti orientation
and conformations of angles less than 180° down to 60°
are of less than 1 kcal/mol. Accordingly, this steric
factor cannot account for the low potency of these
compounds. One may conclude that the relatively high
conformational flexibility of PCP allows for adaptation
to an optimal fit at the receptor and, conversely, the
rigidity of analogues such as 13, 14, and 16 prevents
conformational relaxation into the correct conformation
for optimal binding.
a
(ix) KOH, C6H5I(OAc)2, MeOH, -5 °C; (x) (a) LiN(iPr)2,
ClCOOCH3, Et2O, -78 °C, (b) KOH, MeOH, H2O; (xi) P2O5,
CH3SO3H; (xii) C6H5I(OAc)2, I2, AIBN, C6H6 reflux; (xiii) (a) NaOH,
H2O reflux, (b) PCl5, Et2O, (c) NH3, CH2Cl2, -78 °C; (xiv) (a)
C6H5I(OH)OTs, CH3CN reflux, (b) K2CO3, Br(CH2)5Br, DMF
reflux.
Ta ble 1. Inhibition of [3H]TCP and [3H]NANM Bindinga
IC50, µM
relative
potency
PCP/σ
ratio
compd
[3H]TCP
[3H]NANM
PCPb
0.091 ( 0.005
0.53 ( 0.10
1
0.172
0.003
13
MK-801b
14
16
13
0.0053 ( 0.0003 1.7 ( 0.5
4.27 ( 0.02 0.330 ( 0.044
107 ( 7.1
117.5 ( 7.8
17.2
0.02
0.00085 293
0.00077 368
0.365 ( 0.069
0.319 ( 0.058
a
b
Mean ( standard deviation of three experiments. Literature
data for PCP and MK-801 are included for comparison.71
By contrast, rigid analogues 13, 14, and 16 bind to
the σ receptor and are almost twice as potent as PCP
and around 6 times as potent as MK-801. This shows
the virtually inverse requirements for the two sites; MK-
801 possesses an optimal orientation of the bridging
ammonium center with respect to either of the two
phenyl rings and each N-H bond of the protonated
P h a r m a cologica l Resu lts a n d Discu ssion
The results of radioreceptors assays are presented in
Table 1.
The general observation can be made that increased
rigidity of the PCP analogues 13, 14, and 16 leads to
significantly diminished affinity for the PCP site. Ana-
logues 13 and 16 are essentially nonbinding while 14,
which has the Φ ) 60° conformation, is a ligand, albeit
about 50 times less active than PCP at the PCP binding
site.
The relative order of affinity of the three rigid
analogues of PCP agrees with the theoretical conclu-
sions and experimental demonstration by Kozikowski
et al.67 that the Φ ) ca. 90° orientation is the optimal
for binding at the PCP site. Analogue 2, which is
structurally very similar to 14, with the difference being
an extra methylene group in 2 and an N-ethyl group
rather than the piperidine group, shows either a 51%
or 6% relative potency in PCP receptor binding depend-
ing upon the enantiomer.
+
bridging NH2 group has an anti relationship to the
phenyl-bridgehead C-C bond. The three analogues fit
approximately a pharmacophore model which has been
proposed for the σ receptor.68 Figure 3a shows the basic
N-(phenylpropyl)-N-normetazocine structure, which con-
sists of one lipophilic site constituted by the phenolic
group and an ammonium center available as a hydrogen
bonding site connected further to a second lipophilic site
supplied by the N-substituent.
Analogues 13 and 16 fit this pharmacophore as
indicated in parts b and c of Figure 3, respectively. For
analogue 14 (Figure 3d), it is necessary to rotate the
molecule to bring the ammonium center into position
for hydrogen bonding. The site 2 lipophilic center is now
constituted by the cyclohexyl ring. The alternative
orientation, with the same spatial relationship as in
Figure 3a-c, may also be considered, but this leads to
somewhat weaker binding between lipophilic site 1 and
the aromatic ring.
Con clu sion s
Systematic variation in the spatial orientation of the
nitrogen center and aromatic ring in the synthesized
rigid PCP analogues leads to sizable differences in
binding constants to the PCP and NANM receptors.
The relative potencies of these stereoisomers are
considerably greater than 14:PCP ) 2%. Of course, 33

Rigid Phencyclidine Analogues
J ournal of Medicinal Chemistry, 1998, Vol. 41, No. 4 473
(argon or nitrogen). Yields refer to isolated products that were
found to be chromatographically and spectroscopically (1H
NMR) homogeneous materials, unless otherwise stated.
Meth yl 2-(N,N-Dieth yla m in o)-3-cycloh exen e-1-ca r box-
yla te (19). Crotonaldehyde (17) (7.0 g, 0.100 mol) was
converted to 1-(N,N-diethylamino)-1,3-butadiene (18) (6 g, 48%
yield) according to a literature procedure,73 which was followed
by reaction with methyl acrylate (6.4 g, 75 mmol) to give 19
(6.6 g, 65% yield), bp 118-120 °C/10 mmHg (lit.79 bp 130-
132 °C/12 mmHg).
Meth yl 1,3-Cycloh exa d ien e-1-ca r boxyla te (20). The
compound 19 (6.6 g, 31 mmol) was converted to 20 (2.0 g, 44%
yield) according to a literature procedure.73 The product 20
had the following characteristic spectral data: 1H NMR
(CDCl3) δ 7.00 (dd, 1H, CHdCH), 6.13 (m, 1H, CHdCH), 6.07
(m, 1H, CHdCH), 3.76 (s, 3H, CO2CH3), 2.45 (m, 2H, CH2),
2.28 (m, 2H, CH2); 13C NMR (CDCl3) δ 167.9 (CdO), 133.5,
133.2, 127.1, 123.9, 51.6, 22.8, 20.8; IR (neat) 2951, 2880, 2835,
1709, 1269, 1090 cm-1
.
Ben zobicyclo[2.2.2]octen e-1-ca r boxylic Acid (22b). A
mixture of benzenediazonium-2-carboxylate (from 5.6 g of
anthranilic acid and 10 mL of isoamyl nitrite) and methyl 1,3-
cyclohexadiene-1-carboxylate (20) (3.3 g, 24 mmol) was heated
at 70 °C for 15 h. After 200 mL of CH2Cl2 was added, the
mixture was washed (saturated NaHCO3, water) and dried
(MgSO4). The solvent was removed under reduced pressure.
The residue was passed through a flash chromatography
column with silica gel. Elution with hexane/ether (98:2, v/v)
gave 3.0 g of a mixture of the methyl benzobicyclo[2.2.2]-
octadiene-1-carboxylate (21) and methyl 1,3-cyclohexadiene-
1-carboxylate (20).
The mixture was hydrogenated in MeOH under 30 psi in
the presence of 10% palladium charcoal. The catalyst was
removed by filtration. After removal of MeOH, the residue
containing 22a was dissolved in 5 mL of methanol, followed
by addition of KOH (1.2 g, 21 mmol) in 10 mL of water. The
mixture was stirred at room temperature overnight. The
reaction mixture was diluted with water, washed with ether,
followed by acidification of the aqueous layer with 4 N HCl to
pH ) 1, and reextracted with 3 × 100 mL of ether. The
combined ethereal extracts were washed with brine and dried
(MgSO4). After removal of the ether, the residue was recrys-
tallized in hexane to give 22b (2.04 g, 40%): 1H NMR (CDCl3)
δ 7.31-7.23 (m, 5H, aromatic protons), 3.10 (m, 1H, bridgehead
proton), 2.05 (m, 2H, CH2), 1.87 (m, 4H, CH2), 1.52 (m, 2H,
CH2); 13C NMR (CDCl3) δ 181.6 (CdO), 143.2, 140.4, 126.6,
126.1, 123.9, 122.2, 46.8, 34.5, 29.0, 26.0; IR (KBr) 3200-2500
(br, COOH), 1695 (CdO), 1605 (CdC), 700, 659 cm-1; MS (CI)
203 (M + 1, 100), 157 (62), 129 (8).
F igu r e 3. Hypothetical fitting of 13 (b), 16 (c), and 14 (d)
into the pharmacophore model proposed for the σ-receptor in
the case of N-(phenylpropyl)-N-normetazocine (a).
Ben zob icyclo[2.2.2]oct en e-1-ca r b oxa m id e
(22c).
Benzobicyclo[2.2.2]octene-1-carboxylic acid (22b) (2.5 g, 11.8
mmol) was dissolved in dry CH2Cl2, and PCl5 (2.5 g, 11.8 mmol)
was added in several portions. The reaction mixture was
stirred at room temperature for 16 h. The reaction solution
was cooled to -78 °C, ammonia was bubbled into the solution
until pH ) 9, and the solution was allowed to warm to room
temperature. Then, 50 mL of water was added, and the layers
were separated. The organic layer was washed with brine and
dried (MgSO4). After removal of CH2Cl2, the crude product
was recrystallized to give 22c (1.8 g, 76% yield): mp 238-
239 °C; 1H NMR (methanol-d6) δ 8.10 (d, br, 2H, CONH2), 7.97
(m, 4H, aromatic protons), 3.80 (m, 1H, bridgehead proton),
2.60-2.40 (m, 6H, CH2), 2.10 (m, 2H, CH2); 13C NMR (methanol-
d6) δ 178.2 (CdO), 146.1, 144.9, 128.6, 128.3, 126.2, 125.0, 48.7,
35.4, 31.2, 28.5; IR (KBr) 3404, 3192, 2934, 1657, 1116, 754,
698 cm-1; MS (CI) 202 (M + 1, 100), 185, 157, 125, 111.
1-Am in oben zobicyclo[2.2.2]octen e (23). A mixture of
the amide 22c (0.6 g, 3 mmol) and hydroxy(tosyloxy)iodoben-
zene (1.41 g, 3.6 mmol) in 20 mL of dry acetonitrile was
refluxed for 20 h under nitrogen and then cooled to room
temperature. After removal of the acetonitrile, 50 mL of 2 N
HCl was added, and the solution was washed with ether. The
aqueous solution was basified with saturated NaHCO3 and
extracted with ether. The combined ethereal extracts were
Because of the rigid carbocyclic structures of these
molecules, models for selectivity in substrate-receptor
binding were evaluated. These results may potentially
serve as a basis for drug design in the neuropharma-
ceutical area.
Exp er im en ta l Section
Gen er a l. Unless otherwise noted, materials were obtained
from commercial suppliers and were used without further
purification. NMR spectra were recorded on one of the
following instruments: Brucker WP-200 SY (200 MHz) NMR
spectrometer, Brucker AM-400 (400 MHz) NMR spectrometer.
IR spectra were recorded on IBM system 9000 FT-infrared
spectrophotometer. Mass spectra (MS) were recorded on a
HP5985a and MAT 90 system.
Reactions were monitored by analytical thin-layer chroma-
tography using silica gel plates with UV light illumination,
and 7% ethanolic phosphomolybdic acid/heat were used as
developing agent. Merck silica gel (60 Å, 230-400 mesh) was
used for flash chromatography.
All reactions were carried out under anhydrous conditions
using freshly distilled dry solvent in an inert atmosphere

474 J ournal of Medicinal Chemistry, 1998, Vol. 41, No. 4
Moriarty et al.
washed with water and dried (K2CO3). After removal of the
ether, the product was purified by flash chromatography on
silica gel (ether as eluent) and recrystallized from ether-
hexane to give 23 (0.33 g, 64% yield): 1H NMR (CDCl3) δ 7.41
(d, 1H, aromatic proton), 7.27 (t, 1H, aromatic proton), 7.23
(t, 1H, aromatic proton), 7.17 (d, 1H, aromatic proton), 3.00
(m, 1H, bridgehead proton), 1.85 (m, 2H, CH2), 1.78 (m, 2H,
CH2), 1.68 (br, 2H, NH2), 1.52 (m, 2H, CH2), 1.35 (m, 2H, CH2);
13C NMR (CDCl3) δ 145.8, 142.9, 125.9, 123.2, 119.2, 52.3, 35.1,
by a literature procedure.78 The crude product was purified
by vacuum distillation, giving 28a (10 g, 79% yield) as a
colorless oil: bp 147-158 °C/0.6 mmHg (lit. 145-155 °C/0.5
1
mmHg). The H NMR and IR data of the product agreed with
the literature data.78
3-P h en ylcyclopen tan e-1,1-dicar boxylic Acid (28b). The
ester 28a (10.0 g, 39 mmol) was hydrolyzed to 28b by KOH in
aqueous MeOH. The white solid product (7.5 g, 82% yield)
melts with decomposition at 165-170 °C (lit.76 167-169 °C
dec). The 1H NMR and IR data of the acid agreed with the
literature data.
34.2, 26.9; IR (KBr) 3400, 3350, 2943, 2864, 1643, 1608 cm-1
;
MS (CI) 174 (M + 1, 100), 145, 130.
1-P ip er id in oben zobicyclo[2.2.2]octen e (13). A mixture
of 23 (0.300 g, 1.73 mmol), anhydrous K2CO3 (0.382 g, 2.8
mmol), and 1,5-dibromopentane (0.40 g, 1.73 mmol) in 10 mL
of dry DMF was refluxed for 45 min. Then 30 mL of water
was added, and the basic material was converted to hydro-
chloride, shaken with benzene, separated, liberated with
concentrated NH4OH, and reextracted with CH2Cl2. After
concentration, the residue was purified by flash chromatog-
raphy on silica gel (ether:hexane, 1:5 v/v as eluent) and
recrystallized from CH2Cl2-petroleum ether, to give 0.167 g
9-Oxo-6,7,8,9-tetr a h yd r o-5,8-m eth a n o-5H-ben zocyclo-
h ep ten e-8-ca r boxylic Acid (29).76 A mixture of P2O5 (46 g,
0.32 mol) and methanesulfonic acid (308 g, 3.21 mol) was
stirred at room temperature for 2 h followed by addition of
28b (7.5 g, 0.032 mol) and stirring for 4 h. Pouring the
reaction mixture onto ice/water (1 L), followed by extraction
with ether, gave keto acid 29 (6.7 g, 94% yield) as a light
yellowish solid, mp 200-205 °C dec (lit. mp 204-206 °C). The
spectral data were found to be in complete agreement with
the literature data.
1
of 13 (40% yield): mp 55-56 °C; H NMR (CDCl3) δ 7.45 (d,
9-Oxo-6,7,8,9-tetr a h yd r o-5,8-m eth a n o-5H-ben zocyclo-
h ep ten e-8-Iod id e (30). A mixture of 9-oxotetrahydro-5,8-
methano-5H-benzocycloheptene-8-carboxylic acid (29) (1.5 g,
6.9 mmol), diacetoxyiodobenzene (4.48 g, 13.9 mmol), iodine
(2.56 g, 10.4 mmol), and AIBN (0.113 g, 0.69 mmol) in 120
mL of dry benzene was refluxed for 24 h, cooled, and diluted
with CH2Cl2 (100 mL). The reaction mixture was washed
(saturated NaHCO3, water, brine) and then dried (MgSO4).
After removal of the solvent, the residue was purified by flash
chromatography on silica gel with 5:1 hexane:ether as the
eluent, giving 30 (1.9 g, 92% yield) as a white solid, mp 47-
1H, aromatic proton), 7.26 (t, 1H, aromatic proton), 7.15 (m,
2H, aromatic protons), 3.20 (t, 2H, CH2N), 2.95 (m, 1H,
bridgehead proton), 2.25 (t, 2H, CH2N), 1.90-1.25 (m, 14H,
rest CH2 groups); 13C NMR (CDCl3) δ 144.5, 144.0, 125.4, 125.3,
123.3, 122.9, 60.1, 48.6, 33.8, 27.2, 27.1, 26.1, 25.4; IR (KBr)
3017, 2866, 1603, 1161, 787, 632 cm-1; MS (CI) 242 (M + 1,
100). Anal. Calcd for C17H24ClN (13 hydrochloride): C, 73.49;
H, 8.71; N, 5.04. Found: C, 73.44; H, 8.68; N, 5.10.
1-P ip er id in o-7,8-ben zobicyclo[4.2.0]octen e (14). A so-
lution of cyclohexanone (19.6 g, 0.200 mol) and piperidine (34
g, 0.400 mol) in 200 mL of benzene was heated to reflux, and
heating was continued until 1 equiv of water was collected in
a Dean-Stark trap. The solvent was removed from the
reaction mixture, and the residue was distilled to provide the
intermediate enamine 24, 28 g (85% yield), bp 110 °C/10
mmHg (lit.35). To a refluxing solution of 1-piperidinocyclo-
hexene 24 (7.8 g, 47 mmol) and fluorobenzene 25 (4.5 g, 47
mmol) in 200 mL of ether, under nitrogen, was added a
solution of n-butyllithium (19 mL, 47 mmol, 2.5 M in hexane)
in 200 mL of ether for over 90 min. After 20 h of reflux, 400
mL of dilute HCl was added, the layers were separated, and
the aqueous portion was extracted with ether. Addition of
excess NaOH to the aqueous portion, extraction with ether,
and concentration gave the crude product. The crude product
was purified by chromatography on silica gel (pentane-ether,
1:5, as eluent), yielding 14 (1 g, 45% yield): mp 43-44 °C; 1H
NMR (CDCl3) δ 7.19-7.07 (m, 4H, aromatic protons), 3.58 (m,
1H), 2.74 (m, 2H, CH2N), 2.62 (m, 2H, CH2N), 2.11 (m, 1H),
1.97 (m, 1H), 1.82 (m, 2H), 1.61 (m, 5H), 1.43 (m, 5H), 1.22
(m, 1H), 1.10 (m, 1H); 13C NMR (CDCl3) δ 149.0, 145.3, 127.4,
126.6, 122.4, 121.6, 69.5, 48.0, 45.1, 26.0, 25.5, 24.7, 24.5, 19.7,
18.4; IR (KBr) 3071, 2938, 2847, 1454, 754 cm-1; MS (CI) 242
(M+, 100). Anal. Calcd for C17H24ClN (14 hydrochloride): C,
73.49; H, 8.71; N, 5.04. Found: C, 73.54; H, 8.70; N, 5.06.
Met h yl 3-P h en ylcyclop en t a n e-1-ca r b oxyla t e (27).
4-Phenylcyclohexanone (26) (50 g, 0.252 mol) was added to a
solution of KOH (42 g, 0.758 mol) in 700 mL of MeOH at -5
°C, and the resulting solution was stirred at -5 °C for 15 min.
(Diacetoxy)iodobenzene (184 g, 0.572 mol) was added in several
portions at -5 °C in 10 min. After the addition was completed,
the reaction mixture was stirred at -5 to 0 °C for 2 h. Then
1 L of water was added, and the aqueous solution was
extracted with CH2Cl2 several times. The combined extracts
were washed (brine) and dried (MgSO4). After filtration and
removal of the solvent under reduced pressure, the crude
product was purified by flash chromatography on silica gel
(hexane and hexane:ethyl acetate 9:1 as eluent) to give the
ester 27 (20.6 g, 40% yield): mp 83-85 °C (lit.78 mp 83-85
°C). Spectral properties were found to be in agreement with
the literature data.78
1
48 °C; H NMR (CDCl3) δ 8.10 (d, 1H, Ph), 7.58 (t, 1H, Ph),
7.38 (t, 1H, Ph), 7.36 (d, 1H, Ph), 3.57 (m, 1H, bridgehead
proton), 2.33-2.60 (m, 4H), 1.94 (m, 1H), 1.75 (m, 1H) ppm;
13C NMR (CDCl3) δ 193.0 (CdO), 149.9, 134.4, 128.9, 128.7,
127.2, 126.4, 69.8, 51.0, 43.4, 36.7, 32.4, 25.6 ppm; IR (KBr)
3065, 2945, 2870, 1689, 1597, 1456, 1346, 1285, 1196, 1157,
943 cm-1; MS (CI) 300 (M + 1, 11.7), 299 (100), 171 (11.83),
143 (10.23).
Ben zobicyclo[2.2.1]h ep ten e-1-ca r boxylic Acid (31a ). A
mixture of the iodide (30) (1.11 g, 3.72 mmol) and 50 mL of
40% aqueous NaOH solution was refluxed for 48 h, cooled to
room temperature, and diluted with 100 mL of water. The
aqueous solution was washed with ether, acidified with
concentrated HCl, and then extracted (CH2Cl2). The organic
extracts were dried (MgSO4) and concentrated. The crude
product was purified by flash chromatography on silica gel
with 5:1 hexane:ethyl acetate as the eluent, giving 31a 0.350
g (50% yield): mp 89-91 °C (lit.78 90-91 °C); 1H NMR (CDCl3)
δ 7.55 (m, 1H, Ph), 7.10-7.35 (m, 3H, Ph), 3.48 (m, 1H,
bridgehead proton), 2.39 (m, 1H), 2.15 (m, 2H), 1.97 (d, 1H),
1.60 (m, 1H), 1.35 (m, 1H) ppm; 13C NMR (CDCl3) δ 180.9
(CdO), 147.1, 144.3, 126.5, 125.9, 120.8, 120.5, 57.9, 52.2, 43.5,
31.6, 28.4 ppm; IR (KBr) 3046, 2972, 2874, 2631, 1701, 1606,
1460, 1321, 1279, 1207, 1157, 941 cm-1; MS (CI) 188 (M + 1,
69.5), 172 (52.9), 171 (9.44), 160 (100), 157 (15.3), 144 (30),
131 (47.7), 115 (93).
Ben zob icyclo[2.2.1]h ep t en e-1-ca r b oxa m id e
(31b ).
Benzobicyclo[2.2.1]heptene-1-carboxylic acid (31a ) (0.350 g,
1.86 mmol) was dissolved in 25 mL of dry ether, and PCl5
(0.427 g, 2.05 mmol) was added. The reaction mixture was
stirred at room temperature overnight. After removal of the
solvent, the residue was dissolved in 50 mL of dry CH2Cl2.
The solution was added dropwise into a saturated ammonia/
CH2Cl2 solution at -78 °C. The mixture was slowly warmed
to room temperature with stirring and diluted with 100 mL
of CH2Cl2. The organic solution was washed (water, saturated
NaHCO3) and then dried (MgSO4). After removal of the
solvent, the amide (31b) was obtained (0.318 g, 91% yield):
mp 147.5-149 °C; 1H NMR (CDCl3) δ 7.35 (m, 1H, Ph), 7.10-
7.20 (m, 3H, Ph), 6.58 (br, 1H, CONH), 5.87 (br, 1H, CONH),
3.44 (m, 1H, bridgehead proton), 2.26 (m, 1H), 2.07 (m, 1H),
Dim et h yl 3-P h en ylcyclop en t a n e-1,1-d ica r b oxyla t e
(28a ). Compound 27 (10 g, 49 mmol) was converted to 28a
1.96 (m, 1H), 1.89 (d, 1H), 1.45 (m, 1H), 1.28 (m, 1H) ppm; 13
C

Rigid Phencyclidine Analogues
J ournal of Medicinal Chemistry, 1998, Vol. 41, No. 4 475
(7) Fauman, B.; Baker, F.; Coppleson, L. W.; Rosen, P.; Segal, M.
B. Psychosis induced by phencyclidine. J . Am. Coll. Emerg.
Physicians 1975, 4, 223-225.
NMR (CDCl3) δ 175.9 (CdO), 145.8, 126.5, 126.1, 120.9, 119.9,
59.3, 52.8, 43.8, 30.1, 28.5 ppm; IR (KBr) 3370, 3194, 3046,
2945, 2868, 1657, 1622, 1458, 1300, 1262, 1206, 1126, 974
cm-1; MS (CI) 188 (M + 1, 100), 170, 142, 114, 78.
(8) Luisada, P.; Brown, B. I. Clinical management of phencyclidine
psychosis. Clin. Toxicol. 1976, 9, 539-545.
1-Am in oben zobicyclo[2.2.1]h ep ten e (32). A mixture of
amide 31b (0.318 g, 1.70 mmol) and hydroxy(tosyloxy)iodo-
benzene (0.800 g, 2.04 mmol) in 25 mL of acetonitrile was
refluxed overnight. After removal of the solvent, the residue
was dissolved in dilute HCl. The aqueous solution was washed
with ether and then basified with concentrated NH4OH and
extracted (CH2Cl2). The combined extracts were washed with
brine, dried (K2CO3), and concentrated to give 32 (0.250 g, 90%
yield): 1H NMR (CDCl3) δ 7.35 (m, 1H, Ph), 7.10-7.20 (m,
3H, Ph), 3.25 (m, 1H, bridgehead proton), 2.62 (m, 1H), 2.52
(br, 2H, NH2), 1.85 (m, 1H), 1.85 (m, 1H), 1.71 (m, 2H), 1.30
(m, 2H) ppm; 13C NMR (CDCl3) δ 149.0, 147.2, 125.8, 125.7,
120.6, 117.6, 66.8 (CNH2), 57.4, 41.9, 33.9, 29.5 ppm; IR (neat)
3364, 3291, 3047, 3020, 2961, 2868, 1601, 1475, 1306, 1269,
1207, 1174, 968 cm-1; MS m/e 160 (M + 1, 100), 143, 131, 83,
79.
1-P ip er id in oben zobicyclo[2.2.1]h ep ten e (16). A mix-
ture containing the amine (32) (0.250 g, 1.57 mmol), 1,5-
dibromopentane (0.361 g, 1.57 mmol), and anhydrous K2CO3
(0.350 g, 2.51 mmol) in 5 mL of DMF was kept at reflux for 45
min. Water was added, and the basic material was converted
to the hydrochloride salt by addition of dilute HCl. The
aqueous solution was washed with benzene, followed by
liberation of the amine with concentrated NH4OH and extrac-
tion with CH2Cl2. The combined extracts were dried (MgSO4).
After concentration, the residue was purified by flash chro-
matography on silica gel to yield 16 (0.08 g, 22%): mp 47-48
°C (the hydrochloride salt mp 233-235 °C); 1H NMR (CDCl3)
δ 7.3-7.1 (m, 4H, Ph), 3.26 (m, 1H), 2.80 (t, 4H), 2.15 (m, 1H),
2.0 (m, 1H), 1.85 (d, 1H), 1.75-1.50 (m, 7H), 1.30 (m, 2H) ppm;
13C NMR (CDCl3) δ 147.7, 147.1, 125.6, 125.3, 121.0, 120.8,
77.0, 49.9, 47.3, 41.1, 29.0, 28.4, 26.7, 25.0 ppm; IR (neat) 3020,
2930, 2866, 2799, 1477, 1458, 1288, 750, 538 cm-1; MS (CI)
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228 (M + 1, 100), 227, 199, 143, 115. Anal. Calcd for C16H22
-
ClN (16 hydrochloride): C, 72.85; H, 8.41; N, 5.31. Found:
C, 72.81; H, 8.38; N, 5.36.
Biologica l Stu d ies. Ra d ior ecep tor Assa ys. The binding
assays involved displacement of [3H]TCP or [3H]NANM from
tissue homogenate preparation of fresh whole rat brain minus
cerebellum, as previously described by J acobson et al.16b IC50
values were calculated from inhibition curves. The inhibition
constant (Ki) was determined by using the Cheng-Prusoff
equation.81 Experiments were performed in triplicate.
Ack n ow led gm en t. This work was supported by
Grant No. CHE-9520157 from the National Science
Foundation.
Su p p or tin g In for m a tion Ava ila ble: Tables 2 and 3
containing bond lengths and bond angles for compound and
an X-ray structure of 13 (3 pages). Ordering information is
given on any current masthead page.
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