USE OF SEPHADEX LH-20 FOR SOLID-PHASE SYNTHESIS OF OXYTOCIN

Article abstract of DOI:10.1007/BF00596710

The solid-phase synthesis of oxytocin on commercial Sephadex LH-20 is described.The biological activity of the product obtained was 132 AU/mg, and its oxytocin content, according to HPLC, was 28.2percent.It was shown that the amount of oxytocin and the biological activity do not depend on the degree of addition of Gly⁹ to the polymer matrix within the range of 0.6-1.6 mmoles of Gly⁹/g of resin; after the regeneration, the Sephadex LH-20 can be re-used for solid-phase synthesis of oxytocin.