Russian Journal of Bioorganic Chemistry p. 435 - 441 (2008)
Update date:2022-08-03
Topics:
Belyaeva
Smirnova
Lysogorskaya
Oksenoit
Timofeeva
Lozinskii
Filippova
Preparations with different contents of thermolysin were obtained by the immobilization of the enzyme on granulated polyvinyl alcohol cryogel. Their activity and stability in an aqueous medium and in mixtures of polar organic solvents of different composition were investigated. The catalytic properties of the preparations in reactions of peptide bond formation were studied, and the optimal amount of the biocatalyst, the concentrations of initial reagents, and the ratios of organic solvents and water necessary for effective enzymatic peptide synthesis catalyzed by immobilized thermolysin were determined. A series of peptides of the general formula Z-Ala-Ala-Xaa-pNA, where Xaa = Leu, Ile, Phe, Val, or Ala, were synthesized, and the immobilized enzyme was shown to retain substrate specificity in an organic medium.
View MoreShanghai Mio Chemical Co., Ltd
Contact:0086 21- 64401188-622
Address:16 Floor NO.2 Jiefang Building, No. 4855 Dushi Road, 201100 Shanghai, P.R.China
Contact:86-21 60347964
Address:No.1304, West Meilong Road, Minhang District, Shanghai, China
Nantong Auxin Electronic Technology Co., LTD
Contact:86-513-88760026
Address:NO.5-1, Aoxin Road, Haian Hi-tech Development Zone, Jiangsu Province, China
JIN TAN CHENG'EN CHEMICAL CO.,LTD.
Contact:86-519-82116250
Address:NO.102,village Dongfang,conomic development zone
website:http://www.truewingroup.com
Contact:86-311-66699812
Address:NO.600 ZHONGSHAN EAST ROAD SHIJIAZHUANG
Doi:10.1055/s-2007-991075
(2007)Doi:10.1002/jlcr.695
(2003)Doi:10.1080/00304940309355864
(2003)Doi:10.3390/molecules26133991
(2021)Doi:10.1039/JR9650001045
()Doi:10.1016/S0040-4020(97)00063-X
(1997)