J. L. Sacoman et al. / Carbohydrate Research 362 (2012) 21–29
29
calculated from the linear regression obtained from a Hanes–Woolf
plot (Fig. 4).
To evaluate if glucose 6-sulfonate is a substrate for glucose-6-
phosphate dehydrogenase, 100, 200, 300, and 400 M of glucose
6-sulfonate, 488 M of NADP, and 150 mM HEPES buffer pH 7.2
were mixed up to a total volume of 250 L and the reaction mon-
Supplementary data
Supplementary data associated with this article can be found, in
l
l
l
itored by the same method described above.
References
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l
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3.4.2. Detection of glucosamine 6-sulfonate in vivo
For investigation of the possible presence of glucosamine 6-sul-
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