434
A. Zabeer et al. / European Journal of Medicinal Chemistry 41 (2006) 429–434
raised by injecting 1 μg of egg albumin with 100 mg Al(OH)3
in 1 ml saline i.p. Four weeks later blood was drawn from
hearts and serum separated. Serial dilutions of sera 1:2, 1:4,
1:8 up to 1:256 were made and 0.1 ml of each dilution injected
i.d. in the shaved abdominal skin. One week later, animals
were challenged i.v. with 2 mg of egg albumin and 10 mg
Evans blue contained in 1 ml normal saline. Two groups with
five animals in each group were employed. One group served
as control and the animals of other group were treated with test
drug, 100 mg/kg p.o. 1 h before the challenge with antigen.
As per the method of Goose and Blair (Rorke and Holgate
[28]): Effect of test compound against mast cells degranulation
by compound 48/80 was studied. Male Wistar rats of 100 10
g body weight were selected and divided into four groups of
five animals each. Abdominal skin of these animals was
shaved 1 day before. Group 1 injected with 0.1 ml normal sal-
ine i.d. served as the control, group 2 injected with compound
48/80, 5 μg in 0.1 ml saline served as second control and group
3 was treated with test compound 50 mg/kg i.p. 1 h before the
injection of compound 48/80 i.d. 4th group was treated with
Cromolyn sodium 10 mg/kg i.v. 5 min before the injection of
compound 48/80 i.d. These animals were injected 10 min later
Evan’s blue 10 mg in 1 ml saline. Five minutes later the ani-
mals were sacrificed, abdominal skin reflected and the cross
section area of the skin infiltrated with dye was measured.
Explorative sub-acute toxicity with 200 mg/kg p.o. of 95-4
for 21 days in rats was carried out. Gross observation and body
weight were recorded.
Acknowledgement
Authors are thankful to Shree Dharam Raj, Ex-STA, RRL,
Jammu for his technical assistance.
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Rat B.P., heart rate and E.C.G. Rats of 200 10g body
weight anesthetized with sodium pentobarbitone were em-
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Effect on C.N.S. and toxicity study. Groups of Swiss mice
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and 100, 200, 400 and 800 mg/kg p.o. and observed for C.N.S.
and acute toxicity (48 h mortality).