Phenolic 9,10-secosteroids as products of the catabolism of bile acids by a Pseudomonas sp.

Article abstract of DOI:10.1016/S0039-128X(84)90095-3

The obligate aerobe, Pseudomonas putida ATCC 31752, efficiently utilises bile acids as a source of carbon and energy for growth and maintenance.When aeration is considerably restricted, a consequence to the catabolism of the bile acids in a fermentor is an accumulation of certain steroidal catabolites.Evidence is presented to show that among these are hydroxy-9,10-seco-1,3,5(10)-androstatriene-9,17-diones and those from four of the common bile acids, cholic, chenodeoxycholic, hyodeoxycholic and deoxycholic acids have been isolated and their structures determined.The product of catabolism of hyodeoxycholic acid appears to exist in a hemi-acetal form which readily forms an acetal during isolation procedures.All but one of these are described for the first time.