BIOSYNTHESIS OF 2-HYDROXY-3-BUTENYLGLUCOSINOLATE AND 3-BUTENYLGLUCOSINOLATE IN BRASSICA NAPUS

Article abstract of DOI:10.1016/0031-9422(90)85177-H

Desulpho-3-butenylglucosinolate is considered to be a potential late stage precursor in the biosynthesis of (R)-2-hydroxy-3-butenylglucosinolate (progoitrin) and 3-butenylglucosinolate (gluconapin) in Brassica.Chemically synthesized <4,5-3H>(β-D-glucopyranosyl)-4-pentenethiohydroxamic acid when administered to developing seedlings of Brassica napus cv Bienvenu was found to be well incorporated into both 2-hydroxy-3-butenylglucosinolate and 3-butenylglucosinolate, while feeding experiments with <14C>methionine have shown that de novo biosynthesis is slow.Analysis of seeds and developing seedlings has shown that levels of 2-hydroxy-3-butenylglucosinolate are maintained while 3-butenylglucosinolate levels are considerably reduced.