Competitive immunoassay (Cat-EIA), a helpful technique for catalytic antibody detection. Part I

Article abstract of DOI:10.1016/S0040-4039(99)00067-2

A competitive immunoassay procedure for the screening of catalytic antibodies is reported. This screening approach (Cat-EIA) is a modification of the well-known Cat-ELISA technique avoiding the substrate modification step. It has been developed for a bimolecular reaction and has been tested on a high number of hybridoma clones. The current study explores for the first time the utility and feasibility of this method for the early detection of catalytic antibodies.