Communication
ChemComm
difficult to be effectively worked in the absence of a carrier, providing an assembled strategy based on multicharged cyclo-
although its anti-cancer effect was very good. To our dextrin for cancer treatment.
delight, HAAD–AMCD showed a great cancer cell inhibitory
We thank NNSFC (No. 21861132001, 21971127, 21772099)
effect, and the cell viability was only close to 50% in #5 for financial support.
À1
À1
(
[HAAD] = 17.44 mg L , [AMCD] = 237.6 mg L ). We suggested
that the assembly targeted the cancer cells and released AMCD
under the action of hyaluronidase. Then, AMCD selectively
complexed with ATP and formed a stable 1 : 1 AMCD * ATP
assembly to prevent ATP hydrolysis and cut off the energy
supply of cancer cells, which inhibited the normal activities of
Conflicts of interest
There are no conflicts to declare.
cancer cells. Cbl@HAAD–AMCD assembly showed the best effect Notes and references
À1
with the cell viability of 35% in #5 ([HAAD] = 17.44 mg L
[
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À1
À1
AMCD] = 237.6 mg L , [Cbl] = 25.68 mg L ). This further
verified that the hydrophobic anticancer drug was loaded on the
assembly, which thus enhances the effect of basic chemotherapy
in the presence of AMCD. Besides, low cytotoxicity could be
observed on the human embryonic kidney normal cell line 293T
cells (Fig. 3b). Then, confocal laser scanning microscopy experi-
ments were designed to examine the effect of ATP capture on
P-glycoprotein. Calcein-AM can diffuse across the cell mem-
branes by its hydrophobicity, which was utilized as a probe to
test the activity of the efflux pumps in cell membranes by
monitoring the fluorescence intensity. As shown in Fig. 3c,
the fluorescence intensity of the A549 cells in the control
experiment became weaker after culturing the cells for 48 h,
indicating that hydrolyzed calcein was pumped out due to the
ATP hydrolysis. A similar phenomenon was observed for cells
under the same incubation conditions in the presence of HAAD.
While the fluorescence intensity of the cells changes much
slower in the presence of AMCD owing to the inhibition of
ATP hydrolysis forming AMCD * ATP. Of course, the inhibition
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This journal is The Royal Society of Chemistry 2021
Chem. Commun., 2021, 57, 2812À2815 | 2815