Steroids p. 603 - 620 (1984)
Update date:2022-08-17
Topics:
Bain, J. D.
Kasman, L. H.
Bercovitz, A. B.
Lasley, B. L.
The efficiencies for estrogen conjugate hydrolysis were compared between enzyme hydrolysis, acid solvolysis and a new method, ammonolysis.Samples included: 1) crystalline 1,3,5(10)-estratriene-3,17β-diol disulfate (estradiol 3,17-disulfate), 2) squirrel monkey urine collected following an intravenous injection of <2,4,6,7-3H> 1,3,5(10)-estratriene-3,17β-diol (estradiol) and 3) a pool of human pregnancy urine.Ammonolysis demonstrated a significant increase over the other techniques in "free" estrogen yields, specifically, from estradiol 3,17-disulfate.
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