An efficient synthesis of enantiomerically pure Δ- and Λ-ruthenium(II)-labelled oligonucleotides

Article abstract of DOI:10.1002/hlca.19970800303

The synthesis of a novel tris(bidentate ligand)ruthenium(II) complex 7 and its efficient tethering to the 5'-end of oligonucleotides is described. The resulting Δ- and Λ-isomeric ruthenium(II)-labelled oligonucleotides 10a-c and 11a-d were separated either by reversed-phase HPLC or by polyacrylamide gel electrophoresis. The diastereoisomerically pure isomers were fully characterized by UV/VIS and CD spectroscopy, mass spectrometry, and enzymatic digestion with base analysis. We also investigated the thermal denaturation of the hybridized double strands.