IMPROVED DEPROTECTION IN SOLID PHASE PEPTIDE SYNTHEIS: QUANTITATIVE REDUCTION OF METHIONINE SULFOXIDE TO METHIONINE DURING HF CLEAVAGE

Article abstract of DOI:10.1016/S0040-4039(00)87499-7

The reduction of methionine sulfoxide residues in peptides was found to be quantitative by treatment with a low concentration of HF in dimethyl-sulfide (HF:dimethylsulfide, 1:3, v/v).The reduction was dependent on the acidity function of HF and was optimal with an HF concentration between 25percent and 40percent.The low HF concentration cleavage reagent also deprotected most of the benzyl alcohol-derived protecting groups.