Chemical and Pharmaceutical Bulletin p. 3567 - 3570 (1985)
Update date:2022-08-11
Topics:
Matsuura
Yoshino
Ooki
et al.
A high-performance liquid chromatographic (HPLC) method has been developed for the determination of methylglyoxal (MG). When MG was treated with o-phenylenediamine and trichloroacetic acid, it was converted to a highly fluorescent new compound, 2-(2-benzimidazolyl)-3-methylquinoxaline (BIMQ) the chemical structure of which was elucidated by NMR and MS. BIMQ was analyzed by HPLC using a 4 mm x 25 mm column packed with LiChrosorb Si-60 (10 μ). This method is highly sensitive and was satisfactorily applied to the measurement of MG in biological materials.
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