25952-53-8 Usage
Description
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, commonly known as EDC-HCl, is a water-soluble derivative of carbodiimide that is widely utilized in the field of biochemistry and molecular biology. It is a white crystalline powder that is easily deliquescent and soluble in water and ethanol. EDC-HCl is known for its ability to catalyze the formation of amide bonds between carboxylic acids and amines, making it a valuable tool in peptide synthesis and the modification of various biomolecules.
Uses
1. Used in Peptide Synthesis:
EDC-HCl is used as a condensing agent in peptide synthesis, facilitating the formation of amide bonds between carboxylic acids and amines. Its water solubility and the ease of removing excess reagent make it a preferred choice for this application.
2. Used as a Carboxyl Activating Agent:
EDC-HCl plays a crucial role in the activation of carboxyl groups, particularly in the immobilization of large biomolecules in conjunction with N-hydroxysuccinimide (NHS). This activation process is essential for various biological and chemical applications, including the creation of immunocouplers and the acylation of phosphoranes.
3. Used in the Modification of NMDA Receptors:
EDC-HCl is employed in the modification of N-methyl-D-aspartate (NMDA) receptors, which are involved in various neurological processes and disorders. The use of EDC-HCl in this context allows for the investigation of receptor function and the development of potential therapeutic strategies.
4. Used in Cross-linking of Proteins and Nucleic Acids:
EDC-HCl is also utilized in the cross-linking of proteins and nucleic acids, which is an important technique for studying the structure, function, and interactions of these biomolecules.
5. Used in the Production of Immunocouplers:
EDC-HCl is used in the production of immunocouplers, which are essential for the development of immunoassays and other diagnostic tools.
Application Industry:
1. Pharmaceutical Industry:
In the pharmaceutical industry, EDC-HCl is used as a carboxyl activating agent for the synthesis of various drugs and drug delivery systems, as well as in the development of novel therapeutic strategies.
2. Biotechnology Industry:
EDC-HCl is employed in the biotechnology industry for the modification and immobilization of biomolecules, which is crucial for the development of new biotechnological applications and products.
3. Research and Development:
In research and development, EDC-HCl is used for peptide synthesis, modification of NMDA receptors, and the study of protein-nucleic acid interactions, contributing to the advancement of scientific knowledge and the development of new technologies.
Flammability and Explosibility
Flammable
Biochem/physiol Actions
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is a water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. Additionally, it reacts with phosphate groups. It has been utilized in peptide synthesis, crosslinking proteins to nucleic acids as well as preparation of immunoconjugates.
Synthesis
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride can be used for the synthesis of amides.?It is used as a coupling agent in the synthesis?of esters from carboxylic acids using dimethylaminopyridine as the catalyst.
Purification Methods
It is an excellent H2O-soluble peptide coupling reagent. It is purified by dissolving (ca 1g) in CH2Cl2 (10mL) at room temperature and then add dry Et2O (~110mL) dropwise and the crystals that separate are collected, washed with dry Et2O, recrystallised from CH2Cl2/Et2O and dried in a vacuum over P2O5. It is important to work in a dry atmosphere or work rapidly and then dry the solid as soon as possible. The material is moderately hygroscopic, but once it becomes wet it reacts slowly with H2O. Store it away from moisture at -20o to slow down the hydrolysis process. The free base has b 47-48o/0.27mm, 53-54o/0.6mm, n 1.4582. The methiodide is recrystallised from CHCl3/EtOAc, the crystals are filtered off, washed with dry Et2O, recrystallised from CHCl3/Et2O, and dried in vacuo over P2O5, m 93-95o, 94-95o. [Sheehan et al. J Am Chem Soc 87 2492 1965, Sheehan & Cruickshank Org Synth Coll Vol V 555 1973.] § A polymer bound version is commercially available.
Check Digit Verification of cas no
The CAS Registry Mumber 25952-53-8 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,5,9,5 and 2 respectively; the second part has 2 digits, 5 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 25952-53:
(7*2)+(6*5)+(5*9)+(4*5)+(3*2)+(2*5)+(1*3)=128
128 % 10 = 8
So 25952-53-8 is a valid CAS Registry Number.
InChI:InChI=1/C7H17N3.ClH/c1-4-8-9-6-5-7-10(2)3;/h4-7H2,1-3H3;1H/b9-8+;
25952-53-8Relevant articles and documents
1 - Ethyl - (3 - dimethyl aminopropyl) carbodiimide hydrochloride preparation method (by machine translation)
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Paragraph 0025; 0034; 0035; 0039; 0040; 0044, (2019/03/08)
The invention relates to a 1 - ethyl - (3 - dimethyl aminopropyl) carbodiimide hydrochloride preparation method, which belongs to the technical field of pharmaceutical intermediates. The preparation method comprises the reaction generating EDTC, prepared crude EITC, distillation fractionation, to produce an intermediate, make the EDC and to obtain the target product in seven steps. The present invention provides a preparation method, has a high product yield and purity, the operation step is simple, easy to implement, for industrial production. (by machine translation)
SUBSTITUTED DIHYDROCHINAZOLINES HAVING ANTIVIRAL PROPERTIES
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Page/Page column 92-93, (2008/06/13)
The invention relates to substituted dihydrochinazolines of formula (I), methods for the production thereof, and the use thereof for producing medicaments used for treating and/or preventing diseases, particularly as antiviral agents, especially against cytomegaloviruses.
