Nutrient media for plant-parasitic nematodes: VI. Nucleic acids content and nucleotide synthesis in Aphelenchoides rutgersi☆

Add time:09/05/2019    Source:sciencedirect.com

The nucleic acids content of Aphelenchoides rutgersi, Hooper and Myers, was 0.9% DNA and 2.6% RNA dry weight. The DNA contained 29.5% adenine, 29.3% thymine, 22.5% guanine, and 18.8% cytosine, while the RNA was composed of 22.8% adenine, 23.0% uracil, 31.4% guanine, and 22.9% cytosine on a molar basis.The nematodes needed folic acid for reproduction regardless of the presence or absence of nucleic acid supplements in the culture medium. This was shown by including aminopterin, a folic acid antagonist in the culture medium. A 2-hr incubation of nematodes with glycine-14C (U) and orotic-5-3H acid resulted in the incorporation of 3H-label into both DNA and RNA. Only the RNA fraction contained a significant amount of 14C-label. When this RNA was fractionated, the adenine and guanine accounted for the 14C-label, while cytidylic and uridylic acids contained the 3H-label, thereby demonstrating purine and pyrimidine synthesis by A. rutgersi. The incorporation of orotic acid into the pyrimidines was 8 times higher than that of glycine into purines.

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