Studies on soluble ribonucleic acid of rabbit liver III. Preparation and properties of rabbit-liver soluble RNA

Add time:09/07/2019    Source:sciencedirect.com

Soluble RNA has been prepared in relatively large amounts from rabbit liver by a procedure involving extraction of the “pH-5 enzymes” with hot NaCl. The product is highly homogeneous with respect to molecular size and heterogeneous with respect to biological activity inasmuch as it can act as acceptor for at least 15 different activated amino acids.The nucleotide composition of rabbit-liver soluble RNA has been determined quantitatively by column chromatography, under conditions which permit the separation of 5 minor components: (i) 5-hydroxymethylcytidine ribotide; (ii) 6-methylaminopurine ribotide; (iii) 2-dimethylamino-6-hydroxypurine ribotide; (iv) 1-methylguanine ribotide; and (v) 5-ribosyluracil ribotide; in addition to the 4 major ones: cytidylic acid; adenylic acid; guanylic acid; and uridylic acid. The minor components represent approx. 10% of the total nucleotides.Soluble RNA is markedly resistant to enzymic degradation by a variety of RNA-ases. The significance of these findings is discussed.

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