Formal total synthesis of enantiopure tricyclic (s)-myrmicarin alkaloids 217, 215A and 215B

Article abstract of DOI:10.3987/COM-10-12010

We described herein a formal synthesis of the enantiopure tricyclic (4aS)-myrmicarin alkaloids 217, 215A and 215B owing to the obtention of a common pyrroloindolizidine intermediate, starting from a chiral cis-(2S,5R)- disubstituted pyrrolidine. An intramolecular one-pot aldolization- crotonizationaromatization process of a diketo indolizidine constitutes the key step for the formation of the pyrrole ring of the target compound. The Japan Institute of Heterocyclic Chemistry.

Full text of DOI:10.3987/COM-10-12010

HETEROCYCLES, Vol. 81, No. 11, 2010
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HETEROCYCLES, Vol. 81, No. 11, 2010, pp. 2523 - 2537. © The Japan Institute of Heterocyclic Chemistry
Received, 7th July, 2010, Accepted, 13th September, 2010, Published online, 14th September, 2010
DOI: 10.3987/COM-10-12010
FORMAL TOTAL SYNTHESIS OF ENANTIOPURE TRICYCLIC (S)-
MYRMICARIN ALKALOIDS 217, 215A AND 215B
Marco Santarem, Corinne Vanucci-Bacqué,* and Gérard Lhommet*
P. and M. Curie University, IPCM, CNRS-UMR 7201, Heterocyclic Chemistry, 4
Place Jussieu, Case 43, 75252 Paris cedex, France
E-mail: corinne.bacque@upmc.fr; gerard.lhommet@upmc.fr
Abstract – We described herein a formal synthesis of the enantiopure tricyclic
(4aS)-myrmicarin alkaloids 217, 215A and 215B owing to the obtention of a
common pyrroloindolizidine intermediate, starting from a chiral cis-(2S,5R)-
disubstituted pyrrolidine. An intramolecular one-pot aldolization-crotonization-
aromatization process of a diketo indolizidine constitutes the key step for the
formation of the pyrrole ring of the target compound.
INTRODUCTION
Myrmicarins constitute a new family of oligocyclic air-sensitive alkaloids recently isolated by Schröder et
al.1 as the major constituents of the poison gland of the African ant species Myrmicaria opaciventris.
Among the simplest members of this group, tricyclic myrmicarins 217, 215A and 215B possess a
pyrrolo[2,1,5-cd]indolizidine core whose pyrrole ring is substituted both by an ethyl group and either a
propyl or a (Z) or (E)-prop-1-enyl group (Figure 1). So far, only a few syntheses of these alkaloids have
been reported.2 In particular, Vallée and co-workers2b described in 2000 the first non racemic synthesis of
(R)- and (S)-myrmicarins 217. In this synthesis the pyrrole ring was formed early in the sequence by
condensation of D- or L-glutamic acid, as the chiral source, with tetrahydro-2,5-hydroxyfuran. More
recently, Movassaghi and Ondrus2e achieved the total synthesis of the enantiomerically enriched (R)-
myrmicarins 217, 215A and 215B (ee = 85%). Their approach included a stereospecific palladium-
catalyzed N-vinylation of a substituted pyrrole from a vinyl triflate. Actually, all these syntheses2b,2e
featured a common enantiopure tricyclic keto intermediate (1) (Figure 1), that provided rapid access to
pure tricyclic myrmicarins 217, 215A and 215B.
In our continuing effort aimed at the synthesis of enantiopure alkaloids, we describe herein a new
synthesis of pyrroloindolizidine (4aS)-(1), thus achieving a formal synthesis of the three tricyclic (4aS)-
myrmicarins.

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HETEROCYCLES, Vol. 81, No. 11, 2010
O
R1
R2
N
N
N
H
H
H
(R)-myrmicarin 217
( )-myrmicarin 215A : R = H, R = Me
R
1
2
1
( )-
R
( )-myrmicarin 215B : R = Me, R = H
R
1
2
Figure 1
RESULTS AND DISCUSSION
From a retrosynthetic point of view, we envisioned that the pyrrole ring of compound (4aS)-(1) could
stem from the intramolecular cyclization of diketo indolizidine (8aS)-(2) in which the C-3 position would
be substituted by an oxobutyl group (R = Et). The latter could arise from an intramolecular reductive
amination of 2,5-disubstituted pyrrolidine (3) which in turn would stem from enantiopure cis-(2S,5R)-
disubstituted pyrrolidine (4) (Scheme 1). Indeed, we recently described the preparation of the latter
compound that was used as a key intermediate in the formal synthesis of (+)-gephyrotoxin.3
O
O
H
H
A
A
N
3
CO2Me
CO2Me
A
A
N
N
THPO
MeO
C
R
B
OMe
B
N
H
O
HO
Ph
O
S)-1
(4a
2
3
4
Scheme 1
Our approach relies on the formation of the pyrrole ring at the last stage as the key step of our synthesis.
As a preliminary study, we first explored the access to bicyclic A-C substructure via the obtention of 2,3-
dihydro-1H-pyrrolizine (5) (Scheme 2) as a model molecule of tricyclic compound (1). We reasoned that
the bicyclic pyrrole (5) could result from an intramolecular aldolization-crotonization sequence from
diketo derivative (6), followed by subsequent one-pot aromatization.4 Compound (6) was prepared
starting from commercially available methyl pyrrolidineacetate (7) (Scheme 2).
O
O
CO2Me
N
N
N
H
O
5
6
7
Scheme 2

HETEROCYCLES, Vol. 81, No. 11, 2010
2525
We initially converted pyrrolidine (7) into diesters (8) by condensation of methyl -bromopropionate in
66% isolated yield (1:1 mixture of separable isomers). Diethylketo pyrrolidine (6) was then obtained from
8, following a two-step procedure by reacting ethyl Grignard reagent with the corresponding non isolated
Weinreb diamide in 72% overall yield (Scheme 3).
The required intermediate (6) in hand, we next examined the aldolization reaction under basic conditions.
The use of LDA at −78 °C turned out to be ineffective, whereas LHMDS allowed the regioselective
aldolization to afford the expected cyclization product (9) as a single isomer in quantitative yield. The
latter was then submitted to dehydration in acidic medium. Refluxing in toluene in the presence of p-TSA
(0.1 to 1 equivalent) left the product unchanged. Using HCl in refluxing ethanol led to degradation
products. In contrast, when compound (9) was treated under basic conditions with lithium hydroxide
monohydrate in refluxing ethanol, crotonization occurred followed by in situ aromatization leading to the
expected pyrrole derivative (5) in a moderate 30% yield. We then tried to optimize the sequence by using
lithium hydroxide in the initial aldolization reaction,5 thus allowing a one pot three-step process.
Effectively, when reacting with lithium hydroxide, diketopyrrolidine (6) directly afforded pyrrole (5) in
62% yield (Scheme 3). So, we have succeeded in developping a new simple access to 2,3-dihydro-1H-
pyrrolizine (5) which constitutes a properly substituted model for tricyclic compound (1).
O
LHMDS, -78°C, THF,
N
OH
(quant.)
9
Br
1) NHMe(OMe).HCl,
CO2Me
-BuLi, THF
n
LiOH.H2O,
CO2Me
6
7
EtOH, 80°C (30%)
2) EtMgBr, THF
(72% for 2 steps)
N
K2CO3, TBAI, THF
(66%)
CO2Me
O
LiOH.H2O, EtOH, 80°C
(62%)
8
N
5
Scheme 3
The results of this preliminary methodological study in hand, we then turned our attention to the synthesis
of tricyclic compound (4aS)-(1) starting from enantiopure pyrrolidineacetate (4).3
Pyrrolidine (4) was firstly submitted to hydrogenolysis under an hydrogen atmosphere in the presence of
Pd/C as a catalyst, followed by the protection of the amine function of the crude product as a
benzylcarbamate to afford pyrrolidineacetate (10a). However, silica gel column chromatography did not
allow the separation of the latter compound from phenylethanol. To allow easier isolation, compound (4)

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HETEROCYCLES, Vol. 81, No. 11, 2010
was initially subjected to acetylation. This three-step procedure allowed us to isolate expected compound
(10a) in 57% overall yield. Deprotection of the tetrahydropyranyl protected alcohol function was then
performed by treatment with pyridinium para-toluene sulfonate in ethanol to give rise to alcohol (11a) in
84% yield. Swern oxidation of the latter afforded aldehyde (12a) in 71% yield. Horner-Wadsworth-
Emmons condensation of 12a with the reactive 3,3-diethoxy-2-oxopentylphosphonate in refluxing THF in
the presence of KHMDS as the base,6 provided E-unsaturated ketone (13a) in 76% yield. The double
bond was then chemoselectively reduced under an hydrogen atmosphere in the presence of a catalytic
amount of PtO2 to give pyrrolidine (14a) in 74% yield (Scheme 4).
1) AcCl, NEt3, DMAP
PPTS, EtOH
50°C
CO2Me
COR
COR
2) H2, Pd/C, MeOH
THPO
HO
THPO
N
N
N
Cbz
3) CbzCl, K2CO3, CH2Cl2
HO
Cbz
11a
4
Ph
R = OMe
R = Et
(84%)
(57% overall)
(65% overall)
R = OMe 10a
1) NHMe(OMe),HCl, -BuLi, THF
n
2) EtMgBr, THF
11b
10b
(84%)
R = Et
O
(COCl)2, DMSO
then NEt3, CH2Cl2
(MeO)2P(O)CH2COC(OEt)2Et
COR
H2, PtO2
MeOH, rt
O
COR
N
KHMDS, THF
80°C
EtO
OEt
N
Cbz
Cbz
R = OMe 13a (76%)
R = OMe 12a (71%)
12b
13b
(89%)
R = Et
(91%)
R = Et
O
O
COR
H2, Pd/C
COR
50% aq. TFA
CHCl3,
N
COR
EtO
OEt
N
MeOH, rt
N
O
Cbz
Cbz
R = OMe 15a (86 %)
15b
O
R = OMe 14a (74%)
14b
R = OMe 2a (46 %)
2b
R = Et
(93%)
R = Et
(99 %)
R = Et
(50%)
Scheme 4
However, when compound (14a) was submitted to an hydrogen atmosphere either at room temperature or
at 50 °C in the presence of Pd/C as the catalyst, the in situ expected cylization to yield the corresponding
indolizidine did not occur. Pyrrolidine acetate (3), resulting from the hydrogenolysis of the carbamate
moiety of 14a was the only isolated compound (Scheme 5).
O
H2, Pd/C
CO2Me
14a
EtO
OEt
N
H
MeOH, 50°C
(49%)
3
Scheme 5

HETEROCYCLES, Vol. 81, No. 11, 2010
2527
We thought that this lack of reactivity might stem from the bulkiness of the acetal group which would
prevent cyclization of cis 2,5-disubstituted pyrrolidine (3). We thus envisaged to initially deprotect the
keto function of compound (14a) (Scheme 4). This was achieved in presence of aqueous TFA to give rise
to diketopyrrolidine (15a) in 86% yield. The latter was then submitted to an hydrogen atmosphere in the
presence of Pd/C as the catalyst. To our delight, the intramolecular cyclization effectively occurred even
at room temperature to afford indolizidine (2a) in 46% yield (Scheme 4).7 Noteworthy, indolizidine (2a)
was obtained as a single isomer whose stereochemistry at the C-5 center was postulated to be (R),
according to previous results.8
At this stage, to complete our synthesis toward pyrroloindolizidine (1), we had to prepare indolizidine
(2b) (R = Et) by transformation of the ester moiety of 2a into an oxo-1-propyl one, which could be
achieved via the Weinreb amide. Since this strategy required protecting the keto group of indolizidine
(2a), thus increasing the number of steps, we rather planned to install the oxo-2-butyl side chain at an
earlier step, from ester pyrrolidine (10a) (Scheme 4). Ester (10a) was transformed into ketone (10b) by a
two-step procedure in 65% overall yield, via an intermediate Weinreb amide that was treated with ethyl
Grignard reagent. According to the sequence previously descibed from ester analogue (10a), deprotection
of the alcohol function in the presence of PPTS and subsequent Swern oxidation afforded aldhehyde
(12b) in high yield. Chemoselective condensation of 3,3-diethoxy-2-oxopentylphosphonate on the
aldehyde function of 12b gave pyrrolidine (13b) in 89% yield. Successive double bond reduction (H2,
PtO2, 89%) and deprotection of the acetal group (aq. TFA, CHCl3, 93%) allowed the efficient obtention
of triketo derivative (15b). Subsequent hydrogenolysis and in situ cyclization provided the expected
diketo indolizidine (2b) in 50% yield, as a single isomer. This key intermediate in hand, we then
envisaged the ultimate step in line with the reaction we previously studied, i.e. a one-pot aldolization-
crotonization-aromatization reaction to lead to the target pyrroloindolizidine derivative (1) (Scheme 6).
As expected, when treated in the presence of lithium hydroxide monohydrate in refluxing ethanol,
compound (2b) gave rise to the expected compound (S)-(1) in 64% yield. The spectroscopic data of this
20
compound were identical to those reported in the literature and the optical rotation ([]D −65 (c 0.52;
20
CH2Cl2)) was in accordance with the one given for its enantiomer (for (R)-1: []D +64.8 (c 0.50;
CH2Cl2)2b).
O
LiOH.H2O
2b
N
EtOH, 80°C
(64%)
(S)-1
Scheme 6

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HETEROCYCLES, Vol. 81, No. 11, 2010
In conclusion, we have completed an efficient formal synthesis of enantiopure tricyclic (S)-myrmicarin
alkaloids via enantiopure pyrroloindolizidine (S)-(1), in 12 steps and 7.4% overall yield starting from
enantiopure cis-(2S,5R)-disubstituted pyrrolidine 4. Noteworthy, naturally occurring enantiomer (R)-
myrmicarin can be equally prepared according to a similar way starting from (2R,5S)-4 obtained from
(R)-phenylglycinol. Our strategy relies on the obtention of diketoindolizidine (2b), generated by the
intramolecular reductive amination of an enantiopure 2,5-disubstituted pyrrolidine (15b). Compound (2b)
was then successfully submitted to a one-pot aldolization-crotonization-aromatization reaction, as a
method for the formation of the pyrrole ring. This new methodology is currently under generalization in
the Laboratory.
EXPERIMENTAL
General: Unless otherwise specified, materials were purchased from commercial suppliers and used
without further purification. THF was distilled from sodium/benzophenone ketyl immediately prior to use.
CH2Cl2 was distilled from calcium hydride. All reactions were carried out under argon. Thin layer
chromatography analyses were performed on Merck precoated silica gel (60 F254) plates and column
chromatography on silica gel Gerudan SI 60 (40-60m) (Merck). Melting points are uncorrected. IR:
Philips PU 9706. Optical rotation: Perkin-Elmer 341 polarimeter. HMRS were recorded on a JEOL MS
700 mass spectrometer. NMR: Bruker ARX 250 and ARX 400 spectrometers. Spectra were recorded in
1
CDCl3 or C6D6 as solvents. Chemical shifts () were expressed in ppm relative to TMS at  = 0 for H
NMR, and relative to CDCl3 at  = 77.16 in this solvent or to TMS at d=0 in C6D6 for 13C NMR.
Coupling constants (J) are expressed in Hertz.
It is of note that the NMR studies at room temperature in CDCl3 of all benzyl carbamate compounds
showed that these compounds are present as a mixture of rotamers, which renders their NMR
13
spectroscopic description often unclear and difficult, in particular for C spectra. For this reason, these
spectra have equally been recorded in C6D6 at 343 °K, this temperature generally allowing the
coalescence of the 13C signals.
Methyl 2-(2-(2-methoxy-2-oxoethyl)pyrrolidin-1-yl)propanoate (8). To a solution of aminoester (7)
(0.5 g, 3.5 mmol) in THF (30 mL) at 0 °C were added K2CO3 (0.53 g, 3.80 mmol) and
tetrabutylammonium iodide (0.13 g, 0.35 mmol). Methyl 2-bromopropionate (0.43 mL, 3.80 mmol) was
then added dropwise. The reaction mixture was stirred at reflux temperature for 16 h. After cooling to
room temperature, the mixture was filtered and the solvent removed in vacuo. Silica gel column
chromatography (cyclohexane/AcOEt 6:4) allowed the separation of two diastereomers (8a) and (8b) in

HETEROCYCLES, Vol. 81, No. 11, 2010
2529
equimolar amount as pale yellow oils (0.53 g, 66% overall).
1
For 8a: Rf = 0.25; IR (neat) 1675 cm−1; H NMR (CDCl3, 250 MHz) 1.34 (d, J = 7.5 Hz, 3H),
1.53−1.63 (m, 1H), 1.70−1.80 (m, 2H), 1.95−2.09 (m, 1H), 2.26 (dd, J = 7.5, 15 Hz, 1H), 2.56 (dd, J = 5,
15 Hz, 1H), 2.73−2.83 (m, 1H), 2.97−3.04 (m, 1H), 3.22−3.32 (m, 1H), 3.63 (q, J = 7.5 Hz, 1H), 3.66 (s,
13
3H), 3.69 (s, 3H); C NMR (CDCl3, 62.5 MHz) 17.2, 22.9, 31.2, 40.0, 47.3, 51.0, 51.3, 56.5, 57.8,
172.6, 173.8;HRMS (ESI): m/z calcd for C11H20NO4 (MH+) 230.1386, found 230.1389.
For 8b: Rf = 0.24; 1H NMR (CDCl3, 250 MHz)  1.27 (d, J = 7.5 Hz, 3H), 1.50−1.61 (m, 1H), 1.67−1.77
(m, 2H), 1.88−2.00 (m, 1H), 2.25 (dd, J = 10, 15 Hz, 1H), 2.48 (dd, J = 5, 15 Hz , 1H), 2.53−2.64 (m,
13
1H), 2.88−2.96 (m, 1H), 3.26−3.36 (m, 1H), 3.51 (q, J = 7.5 Hz, 1H), 3.62 (s, 3H), 3.66 (s, 3H); C
NMR (CDCl3, 62.5 MHz) 14.5, 23.3, 31,1, 41.0, 50.0, 51.5, 51.7, 57.0, 58.6, 172.7, 174.6.
2-(2-(2-Oxobutyl)pyrrolidine-1-yl)pentan-3-one
(6).
To
a
cooled
solution
of
N,O-
dimethylhydroxylamine hydrochloride (1.70 g, 17.4 mmol) in THF (40 mL) at −78 °C was added
dropwise a 2.5 M n-BuLi solution in hexanes (13.9 mL, 34.8 mmol). The reaction mixture was stirred for
15 min. after removing the cooling bath, then cooled to −78 °C and a solution of compound (8) (0.30 g,
1.30 mmol) diluted in THF (15 mL) was slowly added. After stirring for 1 h at −78 °C, the reaction was
quenched by addition of saturated aqueous solution of NH4Cl (10 mL) and allowed to warm to room
temperature. The aqueous layer was extracted with AcOEt (3 × 10 mL). The organic layer was washed
with brine (10 mL), dried over Na2SO4, filtered and concentrated in vacuo to afford the crude
intermediate diamide. The latter was dissolved in THF (20 mL) and cooled to −78 °C. Ethylmagnesium
bromide (3 M in Et2O, 1.80 mL, 5.56 mmol) was added dropwise and the reaction mixture was stirred at
0 °C for 2 h. The reaction was quenched by addition of saturated aqueous NH4Cl solution (15 mL). The
aqueous layer was extracted with AcOEt. The organic layer was dried over Na2SO4 and concentrated in
vacuo. Silica gel column chromatography (cyclohexane/AcOEt 6:4) yielding diketone (6) as a yellow oil
(0.21g, 72%). IR (neat) 1695 cm−1; 1H NMR (CDCl3, 250 MHz) 0.72−0.92 (m, 6H), 1.12 (d, J = 7.5 Hz,
3H), 1.21−1.34 (m, 1H), 1.54−1.61 (m, 2H), 1.65−1.91 (m, 1H), 2.18−2.45 (m, 6H), 2.59−2.65 (m, 1H),
2.77−2.82 (m, 1H), 3.07−3.18 (m, 1H), 3.39 (q, J = 7.5 Hz, 1H); 13C NMR (CDCl3, 62.5MHz)  7.6 (2C),
15.0, 23.1, 31.6, 32.9, 36.7, 48.5, 49.9, 56.0, 63.8, 210.8, 213.8; HRMS (ESI): m/z calcd for C13H24NO2
(MH+) 226.1801, found 226.1800.
1-(2-Ethyl-2-hydroxy-3-methylhexahdro-1H-pyrrolizin-1-yl)propan-1-one (9). To a solution of
diketone (6) (0.28 g, 1.24 mmol) in THF (10 mL) at −78 °C was added dropwise LHMDS (1 M in Et2O,
1.86 mL, 1.86 mmol). The reaction mixture was stirred for 2h at −78 °C and quenched by addition of

2530
HETEROCYCLES, Vol. 81, No. 11, 2010
acetic acid (1 mL). Saturated aqueous potassium carbonate was added until pH = 9. The aqueous layer
was extracted with CH2Cl2 (2 × 10 mL). The combined organic layer was dried over Na2SO4, filtered and
concentrated in vacuo to yield compound (9) as a colorless oil which was pure enough to be used in the
next step (0.28 g, 100%). IR (neat) 3365, 1710 cm−1; 1H NMR (CDCl3, 250 MHz) 0.72−1.01 (m, 9H),
1.10−1.17 (m, 2H), 1.30−1.95 (m, 4H), 2.38−2.53 (m, 4H), 2.60−2.64 (m, 1H), 2.78−2.99 (m, 1H),
13
3.58−3.71 (m, 1H), 4.30 (ls, 1H); C NMR (CDCl3, 62.5MHz)  7.2, 9.2, 13.0,26.3, 29.4, 32.1, 38.2,
53.2, 62.0, 66.2, 69.1, 85.7, 214.2; HRMS (ESI): m/z calcd for C13H24NO2 (MH+) 226.1801, found
226.1800.
1-(6-Ethyl-5-methyl-2,3-dihydro-1H-pyrrolizin-7-yl)propan-1-one (5). To a solution of diketone (6)
(0.14 g, 0.62 mmol) in EtOH (4 mL) was added lithium hydroxide monohydrate (39 mg, 0.93 mmol). The
reaction mixture was stirred at 80 °C for 18 h. The solvent was removed in vacuo and the residue purified
by silica gel column chromatography (cyclohexane/AcOEt 7:3) to afford pyrrole (5) (80 mg, 62%) as a
1
yellow solid. mp 69 °C; IR (neat) 1642 cm−1; H NMR (CDCl3, 250 MHz) 1.12 (t, J = 7.5 Hz, 3H),
1.16 (m, J = 7.5 Hz, 3H), 2.11 (s, 3H), 2.53 (quint, J = 7.5 Hz, 2H), 2.67 (q, J = 7.5 Hz, 2H), 2.71 (q, J =
13
7.5 Hz, 2H), 3.12 (t, J = 7.5 Hz, 2H), 3.87 (t, J = 7.5 Hz, 2H); C NMR (CDCl3, 62.5 MHz)  8.8, 9.6,
16.2, 19.5, 26.6, 28.2, 34.4, 45.2, 115.3, 121.5, 127.2, 141.4, 196.6 ; HRMS (ESI) m/z calcd for
C13H19NONa (MNa+) 228.1358, found 228.1361.
(2S,5R)-Benzyl 2-(2-methoxy-2-oxoethyl)-5-(2-(tetrahydro-2H-pyran-2-yloxy)ethyl)pyrrolidine-1-
carboxylate (10a). To a solution of compound (4)3 (3.8 g, 9.71 mmol) in CH2Cl2 (95 mL) were
successively added NEt3 (2 mL, 14.5 mmol), DMAP (0.12 g, 0.97 mmol) and acetyl chloride (2.1 mL,
29.1 mmol). The reaction mixture was stirred at room temperature for 16 h. The organic layer was
washed with water (2 × 20 mL) and brine (15 mL), dried over Na2SO4 and concentrated in vacuo. The
crude product was then dissolved in MeOH (150 mL) and subjected to hydrogenation (1 atm) in the
presence of 5% Pd/C (0.78 g) at room temperature for 16 h. The reaction mixture was filtered over a
Celite pad and the residue washed with MeOH. Concentration in vacuo afforded a crude yellow oil,
which was dissolved in CH2Cl2 (100 mL). To the ice-cooled solution was added K2CO3 (1.53 g, 13.3
mmol) followed by dropwise addition of benzyl chloroformate (1.4 mL, 9.8 mmol). The reaction mixture
was stirred at room temperature for 14 h, filtered and concentrated in vacuo. Silica gel column
chromatography (cyclohexane/AcOEt 6:4) afforded compound (10a) (2.3 g, 57% for 3 steps) as a yellow
oil (1:1 mixture of epimers at C-2 center of the THP group). IR (neat) 1732, 1690 cm−1; 1H NMR (C6D6,
400 MHz, 343 °K) 1.22−1.60 (m, 9H), 1.65−1.84 (m, 2H), 2.07−2.24 (m, 1H), 2.27−2.33 (m, 1H),

HETEROCYCLES, Vol. 81, No. 11, 2010
2531
3.00−3.03 (m, 1H), 3.35 (s, 3H), 3.36−3.43 (m, 2H), 3.70−3.84 (m, 2H), 3.90−4.05 (m, 1H), 4.25−4.31
(m, 1H), 4.47−4.59 (m, 1H), 5.09−5.14 (m, 2H), 7.05−7.30 (m, 5H); 13C NMR (C6D6, 100 MHz, 343 °K)
19.2 and 19.3, 25.4, 29.1 and 29.2, 29.5, 30.5, 35.5 and 35.7, 40.0, 50.3, 55.4, 56.7, 61.3 and 61.4, 64.4
and 64.5, 66.3, 98.2 and 98.3, 127.4, 127.7, 128.0, 137.3, 154.6, 170.7; HRMS (ESI): m/z calcd for
C22H31NO6Na (MNa+) 428.2043, found 428.2037.
(2R,5S)-Benzyl 2-(2-hydroxyethyl)-5-(2-methoxy-2-oxoethyl)pyrrolidine-1-carboxylate (11a). To a
solution of compound (10a) (0.30 g, 0.74 mmol) in EtOH (20 mL) was added PPTS (93 mg, 0.37 mmol).
The reaction mixture was stirred at 50 °C for 16 h. The solvent was removed in vacuo and the residue was
dissolved in CH2Cl2 (10 mL). The organic layer was washed with water (2 × 5 mL), dried over Na2SO4,
filtrated and concentrated in vacuo. Silica gel column chromatography (cyclohexane/AcOEt 3:7) afforded
1
compound (11a) (0.2 g, 84%) as a yellow oil. []D −20.8 (c 2.00, CHCl3); IR (neat) 3496 cm−1; H
20
NMR (C6D6, 400 MHz, 343 °K) 1.19−1.25 (m, 1H), 1.43−1.55 (m, 4H), 1.72−1.80 (m, 1H), 2.19−2.25
(m, 1H), 2.82−2.86 (m, 1H), 3.31 (s, 3H), 3.56 (m, 2H), 4.08−4.13 (m, 2H), 5.06 (m, 2H), 7.06−7.27 (m,
5H); 13C NMR (C6D6, 100 MHz, 343 °K) 29.5, 29.8, 38.8, 40.2, 50.3, 55.3, 55.9, 58.9, 66.7, 127.5,
127.7, 128.0, 136.7, 156.2, 170.4; HRMS (ESI) m/z calcd for C17H23NO5Na (MNa+) 344.1468, found
344,1461.
(2S,5R)-Benzyl 2-(2-methoxy-2-oxoethyl)-5-(2-oxoethyl)pyrrolidine-1-carboxylate (12a). To a cooled
solution of oxalyl chloride (0.62 mL of 2M solution in CH2Cl2, 1.24 mmol) in CH2Cl2 (10 mL) at −60 °C
was added dropwise DMSO (0.14 mL, 1.86 mmol). After stirring for 5 min, a solution of alcohol (11a)
(0.2 g, 0.62 mmol) in CH2Cl2 (10 mL) was added dropwise. The reaction mixture was stirred at −60 °C
for 40 min, then NEt3 (0.52 mL, 3.73 mmol) was added. The reaction mixture was allowed to warm to
room temperature over 30 min. Saturated aqueous NaHCO3 solution (20 mL) was added and the aqueous
layer was extracted with AcOEt (3 × 10 mL). The combined organic layers were washed with saturated
aqueous solution of NH4Cl (10 mL), dried over Na2SO4, filtered and concentrated in vacuo. Silica gel
column chromatography (cyclohexane/AcOEt 4:6) afforded compound (12a) (0.14 g, 71%) as an oil.
1
[]D −3.7 (c 0.70, CHCl3); IR (neat) 1752 cm−1; H NMR (CDCl3, 250 MHz, 295 °K, rotamers) 
20
1.63−1.86 (m, 2H), 2.00−2.17 (m, 2H), 2.25−2.41 (m, 2H), 2.61−3.03 (m, 2H), 3.63 and 3.65 (s, 3H),
4.26−4.34 (m, 2H), 5.12 (m, 2H), 7.29−7.34 (m, 5H), 9.63 and 9.75 (s, 1H); 13C NMR (C6D6, 100 MHz,
343 °K) 29.2, 29.6, 39.6, 49.5, 50.5, 53.9, 55.4, 66.7, 127.6, 127.7, 128.1, 137.0, 154.4, 170.6, 198.4;
HRMS (ESI): m/z calcd for C17H21NO5Na (MNa+) 320.1492, found 320.1488.

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HETEROCYCLES, Vol. 81, No. 11, 2010
(2R,5S)-Benzyl
2-((E)-5,5-diethoxy-4-oxohept-2-enyl)-5-(2-methoxy-2-oxoethyl)pyrrolidine-1-
carboxylate (13a). To a solution of KHMDS (0.10 g, 0.53 mmol) in THF (5 mL) at 0 °C was added
dropwise a solution of 3,3-diethoxy-2-oxopentylphosphonate (0.14 g, 0.48 mmol) in THF (5 mL). The
reaction mixture was stirred at 0 °C for 15 min, then a solution of aldehyde (12a) (0.14 g, 0.44 mmol) in
THF (5 mL) was added. Stirring was continued at 0 °C for 10 min then at 80 °C for 16 h. The reaction
was quenched by addition of saturated aqueous solution of NH4Cl (10 mL) and water (5 mL). The
aqueous layer was extracted with Et2O (6 × 5 mL). The combined organic layers were washed with brine
(5 mL), dried over Na2SO4, filtered and concentrated in vacuo. Silica gel column chromatography
(cyclohexane/AcOEt 6:4) afforded compound (13a) (0.62 g, 76%) as a yellow oil. []D +1.4 (c 0.80,
20
CHCl3); IR (neat) 1744, 1735 cm−1; 1H NMR (CDCl3, 250 MHz, 295 °K, rotamers) 0.65 (t, J = 7.5 Hz,
3H), 1.11 (t, J = 7.5 Hz, 6H), 1.55−1.74 (m, 4H), 1.90−2.15 (m, 2H), 2.23−2.43 (m, 2H), 2.62−3.07 (m,
2H), 3.26−3.45 (m, 4H), 3.56 and 3.58 (s, 3H), 3.85−4.02 (m, 1H), 4.08−4.26 (m, 1H), 5.02−5.09 (m, 2H),
13
6.60−6.68 (m, 1H), 6.80−6.92 (m, 1H), 7.22−7.29 (m, 5H); C NMR (C6D6, 100 MHz, 343 °K)  7.3,
15.1, 26.8, 28.5, 29.4, 39.8, 50.4, 55.6, 57.1, 57.8, 66.6, 104.4, 127.6, 127.9, 128.0, 128.2, 137.2, 143.3,
154.6, 170.7, 195.6; HRMS (ESI): m/z calcd for C26H37NO7Na (MNa+) 498.2462, found 498.2446.
(2R,5S)-Benzyl
2-(5,5-diethoxy-4-oxoheptyl)-5-(2-methoxy-2-oxoethyl)pyrrolidine-1-carboxylate
(14a). A solution of compound (13a) (0.15 g, 0.32 mmol) in anhydrous MeOH (10 mL) was subjected to
hydrogenation (1 atm) in the presence of PtO2 (15 mg) at room temperature for 5 h. The mixture was
filtered over a Celite pad and concentrated in vacuo. Silica gel column chromatography
(cyclohexane/AcOEt 6:4) afforded compound (14a) (0.11 g, 74%) as a colorless oil. []D −1.6 (c 0.60,
20
CHCl3): IR (neat) 1737, 1691 cm−1; 1H NMR (CDCl3, 250 MHz, 295 °K)  0.68 (t, J = 7.5 Hz, 3H), 1.15
(t, J = 7.5 Hz, 6H), 1.21−1.57 (m, 3H), 1.59−1.76 (m, 8H), 2.29 (dd, J = 10, 15 Hz, 1H), 2.44−2.63 (m,
2H), 3.24−3.61 (m, 4H), 3.59−3.61 (s, 3H), 3.73−3.82 (m, 1H), 4.10−4.26 (m, 1H), 5.01−5.11 (m, 2H),
13
7.24−7.32 (m, 5H); C NMR (C6D6, 100 MHz, 343 °K) 7.3, 14.9, 19.8, 26.6, 28.8, 29.6, 35.1, 39.0,
40.1, 50.3, 55.5, 56.9, 58.5, 66.3, 104.6, 127.2, 127.5, 127.7, 137.3, 154.6, 170.6, 207.7; HRMS (ESI):
m/z calcd for C26H39NO7Na (MNa+) 500.2619, found 500.2602.
Methyl 2-((2S,5S)-5-(5,5-diethoxy-4-oxoheptyl)pyrrolidin-2-yl)acetate (3). A solution of compound
(14a) (0.10 g, 0.21 mmol) in anhydrous MeOH (10 mL) was subjected to hydrogenation (1 atm) in the
presence of 5% Pd/C (0.1 g) at 60 °C for 16 h. The mixture was filtered over a Celite pad and
concentrated in vacuo. Silica gel column chromatography (CH2Cl2/7N NH3 solution in MeOH 98:2)
afforded compound (3) (0.35 g, 49%) as a colorless oil. []D20 +7.3 (c 3.00, CHCl3); IR (neat) 3368 cm−1;

HETEROCYCLES, Vol. 81, No. 11, 2010
2533
1H NMR (CDCl3, 250 MHz, 295 °K, rotamers) 0.71 (t, J = 7.5 Hz, 3H), 1.15 (t, J = 7.5 Hz, 6H),
1.38−1.43 (m, 4H), 1.49−1.59 (m, 2H), 1.74−1.88 (m, 4H), 2.07 (ls, 1H), 2.42−2.46 (m, 2H), 2.51−2.66
13
(m, 2H), 3.01−3.06 (m, 1H), 3.31−3.55 (m, 5H), 3.65 (s, 3H); C NMR (CDCl3, 62.5 MHz, 295 °K) 
7.7, 15.4, 21.0, 26.9, 30.6, 30.7, 36.6, 39.7, 41.0, 51.4, 54.8, 57.2, 58.9, 104.7, 173.1, 210.2; HRMS
(ESI): m/z calcd for C18H34NO5 (MH+) 344.2431, found 344.2423.
(2S,5S)-Benzyl 2-(4,5-dioxohepthyl)-5-(2-methoxy-2-oxoethyl)pyrrolidine-1-carboxylate (15a). A
mixture of compound (14a) (0.5 g, 1.04 mmol) in CHCl3 (3 mL) and 50% aqueous TFA (6 mL) was
stirred at room temperature for 16 h. The organic layer was decanted. The aqueous layer was extracted
with CHCl3 (3 × 10 mL). The combined organic layer was washed with water (10 mL) and brine (10 mL),
dried over Na2SO4, filtered and concentrated in vacuo. Silica gel column chromatography
(cyclohexane/AcOEt 6:4) afforded compound (15a) (0.36 g, 86%) as a yellow oil. []D − 0.4 (c 3.00,
20
CHCl3); IR (neat) 1698, 1675 cm−1; 1H NMR (CDCl3, 250 MHz, 295 °K, rotamers) 0.97 (t, J = 7.5 Hz,
3H), 1.11−1.25 (m, 2H), 1.48−1.77 (m, 4H), 1.80−2.09 (m, 2H), 2.24 (dd, J = 10, 15 Hz, 1H), 2.54−2.74
(m, 4H), 2.87−3.03 (m, 1H), 3.50−3.60 (m, 3H), 3.70−3.85 (m, 1H), 4.04−4.21 (m, 1H), 4.97−5.08 (m,
13
2H), 7.16−7.27 (m, 5H); C NMR (CDCl3, 62.5 MHz, 295 °K, rotamers) 6.8, 19.6, 29.0, 29.4, 29.9,
34.6 and 34.9, 35.8, 39.9 and 40.4, 51.5, 55.2 and 55.5, 58.2 and 58.8, 66.7, 127.8, 127.9, 128.4, 136.7,
155.0, 171.6, 199.3, 200.0; HRMS (ESI): m/z calcd for C22H29NO6Na (MNa+) 426.1887, found 426.1887.
Methyl 2-((3S,5R,8aS)-5-propionyloctahydroindolizin-3-yl)acetate (2a). A solution of compound
(15a) (0.2g, 0.49 mmol) in MeOH (10 mL) was subjected to hydrogenation (1 atm) in the presence of
10% Pd/C (0.2 g) at room temperature for 16 h. The mixture was filtered over a Celite pad and
concentrated in vacuo at 20 °C. Silica gel column chromatography (cyclohexane/AcOEt 6:4) afforded
compound (2a) (57 mg, 46%) as a colorless oil. []D20 +9.2 (c 0.70, CHCl3); IR (neat) 1740, 1680 cm−1;
1H NMR (CDCl3, 250 MHz, 295 °K)  0.97 (t, J = 7.5 Hz, 3H), 1.22−1.52 (m, 5H), 1.63−2.12 (m, 6H),
13
2.18 (dd, J =10, 15 Hz, 1H), 2.38−2.75 (m, 4H), 2.93 (dd, J = 2.5, 10 Hz, 1H), 3.60 (s, 3H);. C NMR
(CDCl3, 62.5 MHz, 295 °K)  7.7, 24.2, 28.4, 29.4, 30.2, 30.4, 33.9, 40.7, 51.5, 61.2, 66.1, 73.6, 172.7,
213.4; HRMS (ESI): m/z calcd for C14H24NO3 (MH+) 254.1750, found 254.747.
(2S,5R)-Benzyl
2-(2-methylenebutyl)-5-(2-(tetrahydro-2H-pyran-2-yloxy)ethyl)pyrrolidine-1-
carboxylate (10b). To a cooled solution of N,O-dimethylhydroxylamine hydrochloride (0.94 g, 9.62
mmol) in THF (20 mL) at −78 °C was added dropwise a 2.5 M n-BuLi solution in hexanes (7.7 mL, 19.2
mmol). The reaction mixture was stirred for 15 min, then a solution of compound (10a) (0.39 g, 0.96

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HETEROCYCLES, Vol. 81, No. 11, 2010
mmol) in THF (20 mL) was slowly added. After stirring for 2 h at −78 °C, the reaction was quenched by
addition of saturated aqueous solution of NH4Cl (15 mL) and allowed to warm to room temperature. The
aqueous layer was extracted with AcOEt (3 × 15 mL). The organic layer was washed with brine (15 ml),
dried over Na2SO4, filtered and concentrated in vacuo to afford crude intermediate amide (0.4 g). The
latter was dissolved in THF (45 mL) and cooled to −78 °C. Ethylmagnesium bromide (3 M in Et2O, 1.3
mL, 3.68 mmol) was added dropwise and the reaction mixture was stirred at 0 °C for 2 h. The reaction
was quenched by addition of saturated aqueous NH4Cl solution (20 mL). The aqueous layer was extracted
with AcOEt (2 × 10 mL). The organic layer was dried over Na2SO4 and concentrated in vacuo. Silica gel
column chromatography (cyclohexane/AcOEt 6:4) afforded compound (10b) (0.25 g, 65%) as a yellow
oil (1:1 mixture of epimers at C-2 center of the THP group). IR (neat) 1697, 1682 cm−1; 1H NMR (C6D6,
400 MHz, 343 °K) 0.90 (t, J = 8 Hz, 3H), 1.24−1.64 (m, 9H), 1.70−1.78 (m, 1H), 1.82−1.92 (m, 1H),
2.01−2.07 (m, 2H), 2.15−2.2 (m, 2H), 3.00−3.04 (m,1H), 3.35−3.44 (m, 2H), 3.74−3.84 (m, 2H),
13
3.88−4.01 (m, 1H), 4.18−4.26 (m, 1H), 4.53−4.56 (m, 1H), 5.10 (s, 2H), 7.06−7.29 (m, 5H); C NMR
(C6D6, 100 MHz, 343 °K) 7.3, 19.3 and 19.4, 25.5, 29.3 and 29.4, 30.1, 30.7, 35.7 and 35.8, 48.2, 55.1,
56.6, 61.5 and 61.6, 64.6, 64.9, 66.4, 98.4 and 98.5, 127.6, 127.8, 128.2, 137.2, 154.5 and 154.6, 207.3;
HRMS (ESI): m/z calcd for C23H33NO5Na (MNa+) 426.2250, found 426.2248.
(2R,5S)-Benzyl 2-(2-hydroxyethyl)-5-(2-methylenebutyl)pyrrolidine-1-carboxylate (11b). To a
solution of compound (10b) (0.16 g, 0.39 mmol) in EtOH (10 mL) was added PPTS (30 mg, 0.12mmol).
The reaction mixture was stirred at 50 °C for 16 h. The solvent was removed under vacuo and the residue
was dissolved in CH2Cl2 (10 mL). The organic layer was washed with water (2 × 5 mL), dried over
Na2SO4, filtrated and concentrated in vacuo. Silica gel column chromatography (cyclohexane/AcOEt 4:6)
afforded compound (11b) (0.11 g, 84%) as a yellow oil. []D −3.6 (c 2.00, CHCl3); IR (neat) 3450,
20
1
1696 cm−1; H NMR (CDCl3, 250 MHz, 295 °K) 0.98 (t, J = 7.5 Hz, 3H), 1.55−1.68 (m, 4H),
1.96−2.04 (m, 1H), 2.24−2.44 (m, 4H), 3.02−3.09 (m, 1H), 3.54−3.71 (m, 2H), 4.08−4.25 (m, 3H),
13
5.08−5.22 (m, 2H), 7.28−7.35 (m, 5H); C NMR (CDCl3, 62.5 MHz, 295 °K) 7.6, 30.1, 31.1, 36.4,
38.5, 49.1, 54.7, 55.6, 59.0, 67.3, 128.0, 128.2, 128.6, 136.3, 156.5, 209.4; HRMS (ESI): m/z calcd for
C18H26NO4 (MH+) 320.1856, found 320.1860.
(2S,5R)-Benzyl 2-(2-oxobutyl)-5-(2-oxoethyl)pyrrolidine-1-carboxylate (12b). To a cooled solution of
oxalyl chloride (0.31 mL of 2 M solution in CH2Cl2, 0.62 mmol) in CH2Cl2 (2 mL) at −60 °C was added
dropwise DMSO (66 mL, 0.94 mmol). After stirring for 5 min, a solution of alcohol (11b) (0.1 g, 0.31
mmol) in CH2Cl2 (2 mL) was added dropwise. The reaction mixture was stirred at −60 °C for 40 min,

HETEROCYCLES, Vol. 81, No. 11, 2010
2535
then NEt3 (0.26 mL, 1.88 mmol) was added. The reaction mixture was allowed to warm to room
temperature over 30 min. Saturated aqueous NaHCO3 solution (15 mL) was added and the aqueous layer
was extracted with AcOEt (3 × 10 mL). The combined organic layers were washed with saturated
aqueous solution of NH4Cl (10 mL), dried over Na2SO4, filtered and concentrated in vacuo. Silica gel
column chromatography (cyclohexane/AcOEt 4:6) afforded compound (12b) (0.09 g, 91%) as an oil.
[]D20 −7.7 (c 1.00, CHCl3); IR (neat) 1765, 1742 cm−1; 1H NMR (C6D6, 400 MHz, 343 °K)  0.86 (t, J =
8 Hz, 3H), 1.17−1.23 (m, 1H), 1.28−1.33 (m, 1H), 1.53−1.58 (m, 1H), 1.68−1.73 (m, 1H), 1.98 (q, J = 8
Hz, 2H), 2.07−2.14 (m, 2H), 2.56−2.59 (m, 1H), 2.80−2.84 (m, 1H), 4.02−4.05 (m, 1H), 4.09−4.15 (m,
13
1H), 5.01−5.09 (m, 2H), 7.03−7.23 (m, 5H), 9.47 (s, 1H); C NMR (C6D6, 100 MHz, 343°K ) 7.1,
29.5 (2C), 35.7, 47.3, 49.4, 53.5, 54.8, 66.4, 127.1, 127.6, 128.1, 136.8, 154.1, 198.4, 207.0; HRMS
(ESI): m/z calcd for C18H24NO4 (MH+) 318.1699, found 318.1703.
(2R,5S)-Benzyl 2-((E)-5,5-diethoxy-4-oxohept-2-enyl)-5-(2-oxobutyl)pyrrolidine-1-carboxylate (13b).
To a solution of KHMDS (0.12 g, 0.62 mmol) in THF (10 mL) at 0 °C was added dropwise a solution of
3,3-diethoxy-2-oxopentylphosphonate (0.16 g, 0.57 mmol) in THF (5 mL). The reaction mixture was
stirred at 0 °C for 15 min, then a solution of aldehyde (12b) (0.16 g, 0.52 mmol) in THF (5 mL) was
added. Stirring was continued at 0 °C for 10 min then at 70 °C for 16 h. The reaction was quenched by
addition of saturated aqueous solution of NH4Cl (10 mL) and water (5 mL). The aqueous layer was
extracted with Et2O (6 × 5 mL). The combined organic layers were washed with brine (5 mL), dried over
Na2SO4, filtered and concentrated in vacuo. Silica gel column chromatography (cyclohexane/AcOEt 6:4)
afforded compound (13b) (0.2 g, 89%) as a yellow oil. []D20 −2.5 (c 0.60, CHCl3); IR (neat) 1740, 1696
cm−1; 1H NMR (C6D6, 400 MHz, 343 °K)  0.80 (t, J = 8 Hz , 3H), 0.88 (t, J = 8 Hz, 3H), 1.11 (t, J = 8
Hz, 6H), 1.24−1.39 (m, 3H), 1.72−1.79 (m, 1H), 1.86 (q, J = 8 Hz, 2H), 2.02 (q, J = 8 Hz, 2H), 2.15 (dd,
J = 12, 16 Hz, 1H), 2.22−2.31 (m, 1H), 2.40−2.52 (m, 1H), 2.95−2.99 (m, 1H), 3.38−3.50 (m, 4H),
13
3.75−3.84 (m, 1H), 4.16−4.23 (m, 1H), 5.04−5.15 (m, 2H), 6.83−7.07 (m, 2H), 7.12−7.27 (m, 5H); C
NMR (C6D6, 100 MHz, 343 °K)  7.8 (2C), 15.6, 27.3, 29.1, 30.4, 36.4, 38.3, 48.2, 55.7, 57.6, 58.1, 67.1,
105.0, 128.2, 128.4, 128.5, 128.8, 137.7, 143.9, 155.0, 196.2, 207.7; HRMS (ESI): m/z calcd for
C27H39NO6Na (MNa+) 496.2669, found 496.2669.
(2S,5S)-Benzyl 2-(5,5-diethoxy-4-oxoheptyl)-5-(2-oxobutyl)pyrrolidine-1-carboxylate (14b). A
solution of compound (13b) (0.22 g, 0.45 mmol) in anhydrous MeOH (10 mL) was subjected to
hydrogenation (1 atm) in the presence of PtO2 (22 mg) at room temperature for 16 h. The mixture was
filtered over a Celite pad and concentrated in vacuo. Silica gel column chromatography

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HETEROCYCLES, Vol. 81, No. 11, 2010
(cyclohexane/AcOEt 6:4) afforded compound (14b) (0.20 g, 93%) as a colorless oil. []D +2.2 (c 0.50,
20
CHCl3); IR (neat) 1733, 1669 cm−1; 1H NMR (C6D6, 400 MHz, 343 °K)  0.75 (t, J = 8 Hz, 3H), 0.89 (t,
J = 8 Hz, 3H), 1.11 (t, J = 8 Hz, 6H), 1.27−1.65 (m, 7H), 1.79-1.85 (m, 5H), 2.03 (q, J = 8 Hz, 2H), 2.20
(dd, J = 8, 16 Hz, 1H), 2.61 (t, J = 8 Hz, 2H), 3.00−3.04 (m, 1H), 3.35−3.39 (m, 2H), 3.75−3.85 (m, 1H),
4.22−4.30 (m, 1H), 5.12 (s, 2H), 7.07−7.30 (m, 5H); 13C NMR (C6D6, 100 MHz, 343 °K)  7.1, 7.2, 14.9,
19.8, 26.6, 28.8, 29.9, 35.1, 35.6, 38.9, 48.0, 55.0, 56.8, 58.3, 66.2, 104.5, 127.4, 127.6, 128.0, 137.2,
154.5, 207.1, 207.7; HRMS (ESI): m/z calcd for C27H41NO6Na (MNa+) 498.2826, found 498.2811.
(2S,5S)-Benzyl 2-(4,5-dioxoheptyl)-5-(2-oxobutyl)pyrrolidine-1-carboxylate (15b). A mixture of
compound (14b) (0.25 g, 0.50 mmol) in CHCl3 (2 mL) and 50% aqueous TFA (3 mL) was stirred at room
temperature for 16 h. An aqueous saturated Na2CO3 solution was added (15 mL) and the aqueous layer
was extracted with CH2Cl2 (3 × 10 mL). The combined organic layer was washed with water (10 mL) and
brine (10 mL), dried over Na2SO4, filtered and concentrated in vacuo. Silica gel column chromatography
(cyclohexane/AcOEt 6:4) afforded compound (15b) (0.20 g, 99%) as a yellow oil. []D −6.5 (c 1.00,
20
CHCl3); IR (neat) 1765, 1727 cm−1; 1H NMR (C6D6, 400 MHz, 343 °K)  0.82−0.92 (m, 6H), 1.14−1.56
(m, 7H), 1.64−1.87 (m, 2H), 1.89−2.20 (m, 4H), 2.43−2.54 (m, 2H), 2.87−3.08 (m, 1H), 3.60−3.77 (m,
1H), 4.15−4.31 (m, 1H), 5.07−5.11 (m, 2H), 7.07−7.29 (m, 5H); 13C NMR (CDCl3, 62.5 MHz, 295 °K) 
6.8, 7.6, 19.6, 28.9 and 29.2, 29.45, 29.9 and 30.5, 34.6 and 34.9, 35.8, 36.3, 48.2 and 48.5, 54.5 and 55.2,
57.9 and 58.5, 66.7, 127.8, 127.9, 128.4, 136.7, 155.1, 199.5, 200.1, 209.7; HRMS (ESI): m/z calcd for
C23H32NO5 (MH+) 402.2275, found 402.2271.
1-((3S,5R,8aS)-5-Propionyloctahydroindolizin-3-yl)butan-2-one (2b). A solution of compound (15b)
(0.2 g, 0.49 mmol) in MeOH (10 mL) was subjected to hydrogenation (1 atm) in the presence of 5% Pd/C
(40 mg) at room temperature for 16 h. The mixture was filtered over a Celite pad and concentrated in
vacuo at 20 °C. Silica gel column chromatography (cyclohexane/AcOEt 6:4) afforded compound (2b) (60
mg, 50%) as a yellow oil. []D20 −4.5 (c 1.00, CHCl3); IR (neat) 1695 cm−1; 1H NMR (CDCl3, 250 MHz,
295 °K)  0.92 (t, J = 7.5 Hz, 3H), 0.94 (t, J = 7.5 Hz, 3H), 1.20−1.43 (m, 6H), 1.69−2.11 (m, 6H),
13
2.25−2.41( (m, 4H), 2.46−2.75 (m, 2H), 2.92 (dd, J = 2.5, 10 Hz, 1H); C NMR (CDCl3, 62.5 MHz,
295 °K)  7.7 (2C), 24.4,28.4, 29.9, 30.3, 30.7, 34.0, 36.5, 48.9, 60.1, 66.1, 73.4, 210.8, 213.2; HRMS
(ESI): m/z calcd for C15H26NO2 (MH+) 252.1958, found 252.1955.
(4aS)-1-(1-Ethyl-3,4,4a,5,6,7-hexahydropyrrolo[2,1,5-c,d]indolizin-2-yl)propan-1-one (1).2b To a
solution of compound (2b) (60 mg, 0.24 mmol) in EtOH (4 mL) was added lithium hydroxide

HETEROCYCLES, Vol. 81, No. 11, 2010
2537
monohydrate (15 mg, 0.36 mmol). The reaction mixture was stirred at 80 °C for 18 h. The solvent was
removed in vacuo and the residue purified by silica gel column chromatography (cyclohexane/AcOEt 7:3)
to afford compound (1) (35 mg, 64%) as a yellow solid. mp 72-73 °C; []D −65 (c 0.52, CH2Cl2); IR
20
(neat) 1650 cm−1; 1H NMR (CDCl3, 250 MHz, 295 °K)  1.12 (t, J = 7.5 Hz, 3H), 1.15 (t, J = 7.5 Hz, 3H),
1.18−1.45 (m, 1H), 1.65−1.83 (m, 1H), 1.99−2.14 (m, 3H), 2.45−2.74 (m, 7H), 3.01−3.07 (m, 2H),
13
3.81−3.93 (m,1H); C NMR (CDCl3, 62.5 MHz, 295 °K) 8.7, 15.5, 19.3, 19.6, 22.3, 28.3, 29.9, 34.2,
63.1, 56.2, 116.1, 121.2, 125.0, 138.0, 196.7; HRMS (ESI): m/z calcd for C15H22NO (MH+) 232.1695,
found 232.1691.
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