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7.28–7.37 (m, 3H), 7.11 (d, J = 8.8 Hz, 1H), 6.78 (d, J = 8.8 Hz, 1H), 6.42 (s, 1H),
4.59 (s, 2H), 4.46 (s, 2H), 2.50 (s, 3H); 13C NMR (100 MHz, DMSO-d6) d 165.0,
152.5, 151.9, 148.5, 147.1, 143.3, 137.5, 136.5, 136.1, 134.8, 131.7, 124.3, 121.3,
113.1, 112.1, 55.3, 54.0, 24.8; HRMS (ESI) m/z Calcd for C18H16NO4 310.1079
[MÀH]À, Found 310.3124. (b) 5,6-Dihydroxy-2-[(3-methyl-benzylamino)-
methyl]-chromen-4-one (3n): 1H NMR (400 MHz, CD3OD) d 7.33–7.39 (m,
3H), 7.27–7.29 (m, 1H), 7.10 (d, J = 8.8 Hz, 1H), 6.77 (d, J = 8.8 Hz, 1H), 6.37 (s,
1H), 4.51 (s, 2H), 4.37 (s, 2H), 2.39 (s, 3H); 13C NMR (100 MHz, DMSO-d6) d
164.9, 152.5, 151.9, 148.5, 147.1, 143.5, 137.0, 136.5, 135.3, 134.2, 133.0, 124.3,
121.2, 113.0, 112.1, 56.6, 55.0, 26.5; HRMS (ESI) m/z calcd for C18H16NO4
310.1079 [MÀH]À, Found 310.3372.
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culture plates and cells were lysed in lysis buffer. FRET analysis was
accomplished according to the protocol provided by the 490 HCV protease
assay kit (Anaspec), and the intensity of fluorescence was measured at
340 nmex/490 nmem on a microplate reader.
17. Spectroscopic data for (a) 5,6-dihydroxy-2-[(2-methyl-benzylamino)-methyl]-
chromen-4-one (3g): 1H NMR (400 MHz, CD3OD) d 7.51 (d, J = 7.5 Hz, 1H),