Synthesis, characterization, and biological evaluation of new oxime-phosphazenes

Article abstract of DOI:10.1007/s11164-012-0670-2

Hexachlorocylotriphosphazene (1) was reacted with 4-hydroxy-3- methoxybenzaldehyde to give hexakis[(4-formyl-2-methoxy)phenoxy] cyclotriphosphazene (2). Hexakis[(4-(hydroxyimino)2-methoxy)phenoxy] cyclotriphosphazene (3) was synthesized by reaction of 2 w

Full text of DOI:10.1007/s11164-012-0670-2

Res Chem Intermed (2013) 39:1109–1124
DOI 10.1007/s11164-012-0670-2
Synthesis, characterization, and biological evaluation
of new oxime-phosphazenes
•
Kenan Koran Ahmet Ozkaya Furkan Ozen
•
•
•
Erol Cil Mustafa Arslan
Received: 25 April 2012 / Accepted: 29 May 2012 / Published online: 26 June 2012
Ó Springer Science+Business Media B.V. 2012
Abstract Hexachlorocylotriphosphazene (1) was reacted with 4-hydroxy-3-meth-
oxybenzaldehyde to give hexakis[(4-formyl-2-methoxy)phenoxy]cyclotriphospha-
zene (2). Hexakis[(4-(hydroxyimino)2-methoxy)phenoxy]cyclotriphosphazene (3)
was synthesized by reaction of 2 with hydroxlamine hydrochloride in pyridine.
Compound 3 was reacted with benzyl chloride, acetyl chloride, allyl bromide,
benzoyl chloride, propanoyl chloride, 4-methoxybenzoyl chloride, 2-chlorobenzoyl
chloride, chloroacetyl chloride, methyl iodide, and thiophene-2-carbonyl chloride.
From these reactions, full or partially substituted compounds were obtained, usually
in high yields. Pure or defined products could not be obtained from reaction of 3 with
methacryloyl chloride and O-acetylsalicyloyl chloride. The structures of the com-
1
pounds were determined by elemental analysis, and IR, H, 13C, and 31P NMR
spectroscopy. The synthesized compounds were screened for in-vitro antimicrobial
activity against two Gram-positive bacteria (Staphylococcus aureus and Entero-
coccus faecalis), two gram-negative bacteria (Escherichia coli and Klebsiella
pneumonia), and fungal strains (Aspergillus niger, and Candida albicans) by the agar
well diffusion method. Few compounds had significant activity against both Gram-
positive and Gram-negative bacteria. None of the compounds had antifungal activity
except compounds 7 and 9, which had moderate activity.
K. Koran ꢀ F. Ozen
Chemistry Department, Science Faculty, Firat University, 23169 Elazig, Turkey
A. Ozkaya
Biochemistry Department, Science Faculty, Adiyaman University, 02040 Adiyaman, Turkey
E. Cil (&)
Science Education Department, Education Faculty, Firat University, 23169 Elazig, Turkey
e-mail: cilerol@yahoo.com
M. Arslan
Science Education Department, Education Faculty, Yildiz Technical University, Istanbul, Turkey
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Keywords Hexachlorocyclotriphosphazene ꢀ Phosphazene ꢀ
Oxime-phosphazenes ꢀ Antibacterial ꢀ Antifungal
Introduction
Phosphazenes are compounds that contain a framework of alternating phosphorus
and nitrogen atoms, either in cyclic or linear form. Cyclophosphazenes are an
important family of inorganic ring systems which have traditionally received
attention for two main reasons:
1. to obtain small-molecule phosphazene derivatives; and
2. to produce polymeric phosphazene derivatives.
They are important in the chemistry of heteroatom compounds [1]. In recent years,
phosphazene polymers have attracted substantial attention because they can be
tailored to have a wide variety of physical and chemical properties by changing the
side groups [2]. Phosphazenes have several biomedical properties and applications,
for example strong antitumor activity [3–7]. Their antimicrobial and biological
activity against bacterial and yeast cells have also been studied [8–10]. Other
applications include model compounds for polyphosphazenes, starting materials for
the preparationof cyclolinearand/or cyclomatrix phosphazene substrates, commercial
polymers with carbon backbones containing pendant cyclophosphazene groups,
inorganic hydraulic fluids and lubricants, biologically important substrates, for
example anticancer agents, insect chemosterilants, pesticides and fertilizers, supports
for catalysts, dyes, crown ether phase-transfer catalysts for nucleophilic substitution
reactions, core substrates for dendrimers, thermal initiators for anionic polymerization
reactions, and photosensitive materials [11].
Oximes are imines with the general formula R1R2C=NOH, where R1 is an
organic side chain and R2 may be hydrogen, forming an aldoxime, or another
organic group, forming a ketoxime. Oximes can be synthesized by condensation of
an aldehyde or a ketone with hydroxylamine. Condensation of aldehydes with
hydroxylamine gives aldoximes; ketoximes are produced from ketones and
hydroxylamine. Oxime and dioxime derivatives are very important compounds in
the chemical industry and in medicine [12]. For example, Oxime compounds are
used as antidotes for nerve agents. A nerve agent inactivates acetylcholinesterase
molecules by phosphorylation of the molecule. Oxime compounds can reactivate
acetylcholinesterate by attaching to the phosphorus atom and forming an oxime-
phosphonate which then splits away from the acetylcholinesterase molecule.
Pralidoxime (also known as 2-PAM), obidoxime, and methoxime are examples for
the most effective oxime nerve-agent antidotes [13]. Some oximes and their alkyl,
oxyalkyl and amino derivatives have physiological and biological activity [14, 15].
The literature contains reports on the synthesis of different linear, cyclic, or
polyphosphazenes [16–26]. There are also a large number of literature reports on the
reactions of functional groups on phosphazene substituents [27]. Typical examples
of these include coupling reactions of trimeric phosphazene azides with aryloxy,
alkoxy, and dialkylamino cosubstituents [28], N-vinylic phosphazenes with
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Synthesis, characterization and biological evaluation
1111
azodicarboxylic and acetylenic esters [29], oxime-phosphazene derivatives with
alkyl and acyl substituents [30–33], and polymers from 4-formylphenoxy [34, 35],
maleic [36], and 3,4-methylenedioxyphenoxy substituents [37].
Infections caused by multi-drug resistant bacteria are of major health concern
worldwide. Particularly important are infections caused by the Gram-positive bacteria
Staphylococcus aureus and species of the genus Enterococcus, because of increasing
incidence of infections caused by these microorganisms in hospitals and communities,
and their ability of developing antibiotic resistance to multiple antibiotics. Because of
serious side effects of newly introduced antibacterial agents, for example the semi-
synthetic streptogramins quinupristin/dalfopristin and daptomycin, development of
diverse series of antimicrobials remains a necessity [38]. We planned the synthesis of
some new phosphazene derivatives bearing different oximes, because phosphazene
compounds and oxime derivatives have important biological properties as medical
agents. Such derivatives could have interesting and useful biological properties. We
recently synthesized oxime-phosphazene derivatives. For synthesis of these com-
pounds, we used full and partially substituted products of hexachlorocyclotripho-
sphazene [30–33]. In this study we synthesized a new oxime-phosphazene,
hexakis[(4-(hydroxyimino)2-methoxy)phenoxy]cyclotriphosphazene (3), by reaction
of hexakis[(4-formyl-2-methoxy)phenoxy]cyclotriphosphazene (2) with hydroxyl-
amine hydrochloride in pyridine. We also studied the reactions of 3 with different alkyl
or acyl halides. All the synthesized compounds were tested for antibacterial and
antifungal activity.
Experimental
General remarks
Solvents and other liquids used in the experimental work were dried by
conventional methods. Hexachlorocyclotriphosphazene [N3P3Cl6] (1) was recrys-
tallized from hexane. Other chemicals were used as purchased. Hexakis[(4-formyl-
2-methoxy)phenoxy]cyclotriphosphazene (2) was prepared as described by Tumer
et al. [39]. Reaction of [N3P3Cl6] with the phenol was performed under dry
1
nitrogen. IR spectra were recorded on an Perkin Elmer FTIR spectrometer. H,
13C, and 31P NMR spectra were recorded by use of a Bruker DPX-300
1
spectrometer operating at 300.13, 75.46, and 121.49 MHz, respectively. The H
and 13C NMR chemical shifts were measured by using SiMe4 as internal standard
whereas those for 31P were measured by using 85 % H3PO4 as external standard.
For the NMR studies, the DMSO-d was used as solvent for the compounds 2, 5, 7,
8, 9, 10, 11, and 13. Acetone-d6 was used as solvent for the compounds 3, 4, 6,
and 12. Chemical shifts downfield from the standard were assigned positive d
values. In the results of NMR spectra, s, d, t, and m indicate singlet, doublet,
triplet, and multiplet, respectively. Microanalysis was performed with a Leco 932
CHNS-O instrument.
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Synthesis of compound 2
A mixture of 1 (1.5 g, 4.31 mmol), 4-hydroxy-3-methoxy benzaldehyde (8.6 g,
51.80 mmol), and K2CO3 (7.16 g, 51.80 mmol) in THF (250 mL) was stirred at
0 °C and then reacted at ambient temperature for 48 h. The solvent was removed
under vacuum. The residue was extracted with CH2Cl2 (4 9 25 mL). After removal
of the solvent a white solid (2) was obtained (3.82 g, 85 %). Anal. Calcd. for
C48H42N3O18P3 (MW = 1041.78): C, 55.34; H, 4.06; N, 4.03. Found: C, 55.14; H,
3.98; N, 3.88 %. IR (cm-1): 1693 mC=O, 1187 mP=N, 1285 mP–N–P, 950 mP–O–C
.
31P NMR: 7.82 (s). H NMR: 9.82 (6 H, s, H(8)), 7.12–7.50 (18 H, m, H(3), H(5),
H(6)), 3.80 (18 H, s, H(7)), 2.45 and 3.30 (DMSO-d). 13C NMR: 190.18 C(8), 116.8
C(4), 115.25 C(2), 115.66 C(1), 113.22 C(5), 112.18 C(6), 111.62 C(3), 56.30 C(7),
39.98 (DMSO-d). 2.45 and 3.30 (DMSO-d).
1
Synthesis of compound 3
A mixture of 2 (5 g, 4.80 mmol) and hydroxylamine hydrochloride (2 g,
28.80 mmol) was heated under reflux in pyridine (7 mL) for 3 h. After the reaction
was complete, the mixture was left to cool and was slowly poured into water
(100 mL) and reprecipitated twice from water. The white solid 3 was washed with
alcohol–n-hexane and dried at 50 °C under vacuum. After removal of the solvent a
white solid (3) was obtained (4.34 g, 80 %). Anal. Calcd. for C48H48N9O18P3
(MW = 1131.87): C, 50.93; H, 4.27; N, 11.14 %. Found: C, 50.52; H, 3.99; N,
10.97 %. IR (cm-1): 3304 mOH, 1188 mP=N, 1599, 1587, 1510 mC=N, C=C, 951 mP–O–C
.
31P NMR: 8.71(s). 1H NMR: 10.42 (6 H, s, H(9)), 8.10 (6 H, s, H(8)), 6.88–7.20 (18
H, m, H(3), H(5), H(6)), 3.76 (18 H, s, H(7)), 1.92 and 2.70 (acetone-d6). 13C NMR:
150.92 C(8), 147.80 C(1),140.73 C(2), 130.49 C(4), 121.28 C(5), 119.25 C(6),
109.72 C(3), 55.00 C(7), 29.38 (acetone-d6).
Reaction of 3 with benzyl chloride
A solution of benzyl chloride (0.35 mL, 0.34 g, 2.65 mmol) in acetone (10 mL) was
slowly added dropwise to a stirred and cooled (0–5 °C) mixture of 3 (0.50 g,
0.44 mmol) and K2CO3 (0.74 g, 5.30 mmol) in acetone (30 mL). The reaction was
conducted at room temperature for 1 h then the mixture was heated under reflux for
48 h. After the reaction was complete, the precipitate was isolated by filtration and
the solvent was removed. The product was dissolved in a very small amount of
acetone and was precipitated with hexane. The solvent was removed under vacuum
over 96 h. The clear yellow solid 4 was obtained (0.37 g, 60 %). Anal. Calcd. for
C69H66O18N9P3, (MW = 1402.23). C, 59.10; H, 4.74; N, 8.99 %. Found: C, 58.88;
H, 4.45; N, 8.48 %. IR (cm-1): 3290 mOH, 3060, 3027 mAr–C–H, 2934, 2868 mC–H
(Aliphatic), 1509, 1584, 1598 cm-1
m
C=N,C=C, 1188 mP=N, 950 mP–O–C .31P NMR:
1
8.96(s). H NMR: 10.40 (3 H, s, H(14)), 8.14 (3 H, s, H(8)), 8.11 (3 H, s, H(80)),
6.82–7.57 (33 H, m, H(3), H(30), H(5), H(50), H(6), H(60), H(11), H(12), H(13)),
5.25 (6 H, s, H(9)), 3,75 (9 H, s, H(70)), 3.72 (9 H, s, H(7)), 1.91 and 2.60 (acetone-
13
d6). C NMR: 150,90 C(80), 150,87 C(8), 148,10 C(1), 148,04 C(10), 137,89 C(2),
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Synthesis, characterization and biological evaluation
1113
137,87 C(20), 129,53 C(4), 129,49 C(40), 128,27 C(13), 128,13 C(11), 128,08 C(10),
127,57 C(12), 121,22 C(5), 121,17 C(50), 119,39 C(6), 119,35 C(60), 109,99 C(3),
109,93 C(30), 75.62 C(9), 55.16 C(70), 55.12 C(7), 29.37 (acetone-d6).
Reaction of 3 with acetyl chloride
A solution of acetyl chloride (0.40 mL, 0.45 g, 5.80 mmol) in THF (10 mL) was
slowly added dropwise to a stirred and cooled (0–5 °C) mixture of 3 (0.80 g,
0.84 mmol) and triethylamine (1.55 g, 11.25 mmol) in THF (30 mL). The reaction
was conducted at room temperature for 24 h. When reaction was complete, the
mixture was slowly poured into water (50 mL) and reprecipitated twice from water.
A fawn solid 5 was obtained (0.31 g, 86 %). Anal. Calcd. for C60H60O24N9P3,
(MW = 1384.09). C, 52.07; H, 4.37; N, 9.11 %. Found: C, 51.95; H, 3.97; N,
9.01 %. IR (cm-1): 1766 mC=O, 1193 mP=N, 952 mP–O–C, 3000, 3071 mArC–H, 2967,
2937 mC–H (Aliphatic), 1509, 1599, 1587, 1610 mC=N, C=C.
31P NMR: 8.46(s). 1H NMR:
2.16 (18 H, s, H(10)), 8.53 (6 H, s, H(8)), 6.9–7.33 (18 H, m, H(3), H(5), H(6)), 3.73
(18 H, s, H(7)), 2.46 and 3.30 (DMSO-d). 13C NMR: 168.56 C(9), 155.89 C(8),
150.75 C(1), 141.76 C(2), 128.00 C(4), 121.40 C(5), 120.56 C(6), 111.32 C(3),
56.00 C(7), 19.70 C(10), 39.98 (DMSO-d).
Reaction of 3 with allyl bromide
A solution of allyl bromide (0.2 mL, 0.30 g, 2.30 mmol) in acetone (10 mL) was
slowly added dropwise to a stirred and cooled (0–5 °C) mixture of 3 (0.42 g,
0.37 mmol) and K2CO3 (0.62 g, 4.50 mmol) in acetone (30 mL). The reaction was
conducted at room temperature for 1 h then the mixture was heated under reflux for
24 h. When the reaction was complete, the precipitate formed was isolated by
filtration and the solvent was removed. The product was dissolved in a very small
amount of acetone and was precipitated with hexane. The solvent was removed
under vacuum over 96 h. The yellow solid 6 was obtained (0.43 g, 84 %). Anal.
Calcd. for C66H72 O18N9P3, (MW = 1372.25). C; 57.77; H, 5.29; N, 9.19 %.
Found: C, 57.63; H, 5.14; N, 9.34 %. IR (cm-1): 1188 mP=N, 951 mP–O–C, 3010, 3079
m
ArC–H, 2968, 2921, 2851 mC–H (Aliphatic), 1509, 1599, 1582, 1643 mC=N,
.
C=C
31P NMR: 8.99(s). H NMR: 8.18 (6 H, s, H(8)), 6.8–7.38 (18 H, m, H(3), H(5),
H(6)), 6.08 (6 H, m, H(10)), 5.35 (6 H, d, J 16.40, H(11)trans, 5.20 (6 H, d, J 10.35,
H(11)cis, 4.69 (12 H, d, J 5.64, H(9)) 3.81 (18 H, s, H(7)), 1.90 and 2.74 (acetone-
d6). 13C NMR: 150.90 C(8), 147.84 C(1), 141.01 C(2), 134.50 C(10), 129.60 C(4),
121.26 C(5), 119.40 C(6), 116.40 C(11), 109.75 C(3), 74.50 C(9), 55.00 C(7), 29.37
(acetone-d6).
1
Reaction of 3 with benzoyl chloride
A solution of benzoyl chloride (0.4 mL 0.44 g, 3.2 mmol) in THF (10 mL) was
slowly added dropwise to a stirred and cooled (0–5 °C) mixture of 3 (0.3 g,
0.27 mmol) and triethylamine (0.66 mL, 0.48 g, 4.77 mmol) in THF (30 mL). The
reaction was performed at room temperature for 24 h. When the reaction was
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K. Koran et al.
complete, the mixture was slowly poured into water (50 mL) and reprecipitated
twice from water. A white solid 7 was obtained (0.34 g, 73 %). Anal. Calcd. for
C83H68O23N9P3, (MW = 1652.40). C, 60.33; H, 4.15; N, 7.63 %. Found: C, 60.02;
H, 3.99; N, 7.45 %. IR (cm-1): 3285 mOH, 1746 mC=O, 1189 mP=N, 3010, 3065
m
C–H(Ar), 2972, 2938, 2603 mC–H (Aliphatic), 1508, 1599, 1583, 1610 mC=N, C=C, 950
1
mP–O–C.
31P NMR: 8.50(s). H NMR: 10.61 (1H, s, H(14)), 7.15–7.99 (33 H, m,
H(3), H(30), H(5), H(50), H(6), H(60), H(11), H(12), H(13)), 3.76–3,78 (18 H, s,
H(7), H(70)), 8.82–8,79 (6 H, s, H(8), H(80)), 2.45 and 3.32 (DMSO-d). 13C NMR:
167.60 C(9), 157.46 C(8), 157.41 C(80), 150.85 C(1), 150.82 C(10), 142.77 C(2),
142.73 C(20), 133.13 C(13), 131.02 C(10), 129.50 C(11), 129.18 C(12), 128.83
C(4), 128.80 C(40), 121.72 C(5), 121.69 C(50), 118.60 C(6), 118.58 C(60), 111.47
C(3), 111.44 C(30), 56.59 C(7), 56.09 C(70), 39.97 (DMSO-d).
Reaction of 3 with propanoyl chloride
A solution of propanoyl chloride (0.35 mL, 0.35 g, 4.0 mmol) in THF (10 mL) was
slowly added dropwise to a stirred and cooled (0–5 °C) mixture of 3 (0.4 g,
0.35 mmol) and triethylamine (0.60 mL, 0.42 g, 4.24 mmol) in THF (30 mL). The
reaction was performed at room temperature for 48 h. When the reaction was
complete, the mixture was slowly poured into water (50 mL) and reprecipitated
twice from water. A white solid 8 was obtained (0.39 g, 76 %). Anal. Calcd. for
C66H72O24N9P3, (MW = 1468.24). Calcd.: C, 53.99; H, 4.94; N: 8.59 %. Found: C,
53.52; H, 4.61; N, 8.27 %. IR (cm-1): 1764 mC=O, 1190 mP=N, 3000, 3070 mC–H(Ar)
,
2980, 2941 mC–H (Aliphatic), 1509, 1598, 1584 mC=N, C=C, 950 mP–O–C
.
31P NMR:
1
8.55(s). H NMR: 8.53 (6 H, s, H(8)), 6.95–7.30 (18 H, m, H(3), H(5), H(6)), 2.5
(12 H, m, H(10)), 2.13–3.81 (18 H, s, H(7)), 1.00–1.18 (18 H, t, H(11)), 2.47 and
3.32 (DMSO-d). 13C NMR: 171.60 C(9), 155.99 C(8), 150.77 C(1), 141.72 C(2),
137.34 C(4), 128.05 C(5), 121.40 C(6), 111.33 C(3), 56.50 C(7), 25.70 C(10), 9.10
C(11), 39.97 (DMSO-d).
Reaction of 3 with 4-methoxybenzoyl chloride
A solution of 4-methoxybenzoyl chloride (0.45 g, 2.65 mmol) in acetone (10 mL)
was slowly added dropwise to a stirred and cooled (0–5 °C) mixture of 3 (0.4 g,
0.35 mmol) and K2CO3 (0.58 g, 4.24 mmol) in acetone (30 mL). The reaction was
performed at room temperature for 48 h. When the reaction was complete, the
precipitate was isolated by filtration and the solvent was removed. The product was
dissolved in a very small amount of acetone and was precipitated with hexane. The
solvent was removed under vacuum over 96 h. A white solid 9 was obtained
(0.54 g, 79 %). Anal. Calcd. for C96H84O30N9P3, (MW = 1936.66). C, 59.54; H,
4.37; N, 6.51 %. Found: C, 59.06; H, 4.08; N, 6.12 %. IR (cm-1): 1789 mC=O, 1190
m
P=N, 3010, 3065 mC–H(Ar), 2930, 2845 mC–H (Aliphatic), 1606, 1579, 1510 mC=N, C=C,
1
950 mP–O–C.
31P NMR: 8.62(s). H NMR: 8.74 (6 H, s, H(8)), 3.84 (18 H, s, H(7)),
3.78 (18 H, s, H(14)), 6.94–8.04 (42 H, m, H(3), H(5), H(6), H(11), H(12)), 2.44 and
3.33 (DMSO-d). 13C NMR: 167.31 C(9), 163.25 C(13), 156.97 C(8), 150.82 C(1),
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Synthesis, characterization and biological evaluation
1115
141.84 C(2), 132.98 C(11), 131.62 C(4), 128.04 C(10), 121.55 C(5), 120.37 C(6),
114.40 C(12), 111.38 C(3), 57.00 C(7), 56.00 C(14), 39.98 (DMSO-d).
Reaction of 3 with 2-chlorobenzoyl chloride
A solution of 2-chlorobenzoyl chloride (0.40 mL, 0.56 g, 3.20 mmol) in THF
(10 mL) was slowly added dropwise to a stirred and cooled (0–5 °C) mixture of 3
(0.3 g, 0.27 mmol) and triethylamine (0.66 mL, 0.48 g, 4.77 mmol) in THF
(30 mL). The reaction was performed at room temperature for 24 h. When the
reaction was complete, the mixture was slowly poured into water (50 mL) and
reprecipitated twice from water. A white solid 10 was obtained (0.23 g, 45 %).
Anal. Calcd. for C76H60O22N9P3Cl4, (MW = 1686.07). C, 54.1; H, 3.59; N, 7.48 %.
Found: C, 54.01; H, 3.48; N, 7.32 %. IR (cm-1): 3330 mOH, 1757 mC=O, 1190 mP=N
3000, 3070 mC–H(Ar), 2938, 2976 mC–H (Aliphatic), 1591, 1508 mC=N, C=C, 950 mP–O–C
,
.
31P NMR: 8.37(s). 1H NMR: 10.3 (2 H, s, H(16)), 7.38–7.76 (34 H, m, H(3), H(30),
H(5), H(50), H(6), H(60), H(12), H(13), H(14), H(15)), 3.76–3.78 (18 H, s, H(7),
H(70)), 8.77–8.50 (6 H, s, H(8), H(80)), 2.45 and 3.30 (DMSO-d). 13C NMR: 162.88
C(9), 157.86 C(8), 151.80 C(80), 150.88 C(1), 149.91 C(10), 142.90 C(2), 141.68
C(20), 140.50 C(11), 138.16 C(13), 133.84 C(15), 131.60 C(12), 130.29 C(40),
129.20 C(4), 127.60 C(14), 125.40 C(10), 121.30 C(5), 121.55 C(50), 119.35 C(60),
118.57 C(6), 111.58 C(3), 109.88 C(30), 56.57 C(7), 56.12 C(70), 39.98 (DMSO-d).
Reaction of 3 with chloroacetyl chloride
A solution of chloroacetyl chloride (0.30 mL, 0.46 g, 3.67 mmol) in THF(10 mL)
was slowly added dropwise to a stirred and cooled (0–5 °C) mixture of 3 (0.3 g,
0.27 mmol) and triethylamine (0.66 mL, 0.48 g, 4.77 mmol) in THF (30 mL). The
reaction was performed at room temperature for 24 h. When the reaction was
complete, the mixture was slowly poured into water (50 mL) and reprecipitated
twice from water. A brown solid 11 was obtained (0.34 g, 80 %). Anal. Calcd. for
C60H54O24N9P3Cl6, (MW = 1590.75). C, 45.30; H, 3.42; N, 7.92 %. Found: C,
45.03; H, 3.13; N, 7.77 %. IR (cm-1): 1778 mC=O, 1190 mP=N, 3000, 3076 mC–H(Ar)
,
2938, 2976 mC–H (Aliphatic), 1598, 1587, 1509 mC=N, C=C, 952 mP–O–C
.
31P NMR:
1
8.17(s). H NMR: 8.65 (6 H, s, H(8)), 6.83–8.15 (18 H, m, H(6), H(3), H(5)) 4.67
(12 H, s, H(10)), 3.75 (18 H, s, H(7)), 2.46 and 3.34 (DMSO-d). 13C NMR: 168.88
C(9), 157.16 C(8), 150.90 C(1), 142.96 C(2), 130.61 C(4), 121.56 C(5), 118.89
C(6), 111.69 C(3), 56.09 C(7), 40.76 C(10), 39.97 (DMSO-d).
Reaction of 3 with methyl Iodide
A solution of methyl iodide (0.25 mL, 0.60 g, 4.18 mmol) in acetone (10 mL) was
slowly added dropwise to a stirred and cooled (0–5 °C) mixture of 3 (0.5 g,
0.44 mmol) and K2CO3 (0.74 g, 5.30 mmol) in acetone (30 mL). The reaction was
performed at room temperature for 1 h and was then heated under reflux for 24 h.
When the reaction was complete, the precipitate was isolated by filtration and the
solvent was removed. It was dissolved in a very small amount of acetone and was
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precipitated with hexane. The solvent was removed under vacuum over 96 h. A
clear yellow solid 12 was obtained (0.5 g, 93 %). Anal. Calcd. for C54H60O18N9P3,
(MW = 1216.02). C, 53.34; H, 4.97; N, 10.37 %. Found: C, 53.08; H, 4.73; N,
10.23 %. IR (cm-1): 1188 mP=N, 3000, 3080 mC–H(Ar), 2936, 2960 mC–H (Aliphatic)
,
1599, 1581, 1509 mC=N, C=C, 951 mP–O–C.
31P NMR: 8.94(s). 1H NMR: 8.08 (6 H, s,
H(8)), 6.90–7.24 (18 H, m, H(6), H(3), H(5)), 3.90 (18 H, s, H(9)), 3.80 (18 H, s,
H(7)), 1.92 and 2.71 (acetone-d6). 13C NMR: 150.91 C(8), 147.49 C(1), 140.98
C(2), 129.62 C(4), 121.23 C(5), 119.65 C(6), 109.84 C(3), 61.00 C(9), 55.18 C(7),
29.38 (acetone-d6).
Reaction of 3 with thiophene-2-carbonyl chloride
A solution of thiophene-2-carbonyl chloride (0.30 mL, 0.31 g, 2.12 mmol) in THF
(10 mL) was slowly added dropwise to a stirred and cooled (0–5 °C) mixture of 3
(0.4 g, 0.35 mmol) and triethylamine (0.60 mL, 0.42 g, 4.24 mmol) in THF
(30 mL). The reaction was performed at room temperature for 24 h. When the
reaction was complete, the mixture was slowly poured into water (50 mL) and
reprecipitated twice from water. A clear yellow solid 13 was obtained (0.49 g,
77 %). Anal. Calcd. for C68H56O22N9P3S4, (MW = 1572.41). C, 51.94; H, 3.59; N,
8.02 %. Found: C, 51.75; H, 3.37; N, 7.53 %. IR (cm-1): 3290 mOH, 1736 mC=O
1186 mP=N, 3010, 3087 mC–H(Ar), 2936, 2850 mC–H (Aliphatic), 1626, 1597, 1511
C=N,C=C, 952 mP–O–C
31P NMR: 8.14(s). 1H NMR: 10.60 (2 H, s, H(14)), 8.80 (6 H,
,
m
.
s, H(8), H(80)), 6.88–8.02 (30 H, m, H(3), H(5), H(6), H(30), H(50), H(60), H(11),
H(12), H(13)), 3.75 (18 H, s, H(7), H(70)), 2.46 and 3.35 (DMSO-d). 13C NMR:
163.19 C(9), 158.91 C(8), 151.02 C(80), 150.93 C(1), 148.93 C(10), 142.64 C(2),
141.94 C(20), 134.82 C(10), 133.51 C(11), 131.23 C(4), 130.40 C(40), 121.42 C(5),
121.30 C(50), 128.72 C(13), 125.42 C(12), 119.20 C(60), 118.51 C(6), 109.65 C(30),
109.09 C(3), 56.58 C(7), 56.11 C(70), 39.98 (DMSO-d).
In-vitro antimicrobial assay
The bacterial pathogens S. aureus, E. faecalis, Escherichia coli, and Klebsiella
pneumonia and the fungi A. niger and C. albicans were used for antimicrobial
activity studies. The synthesized compounds were evaluated for antimicrobial
activity by the agar well diffusion method [40, 41]. The bacteria and fungi were
tested on nutrient agar and Sabouraud dextrose agar media, respectively. The
medium was sterilized by autoclaving at 120 °C. Approximately 30 ml agar
medium with the respective strains of bacteria and fungi was transferred aseptically
into each sterilized Petri dish. The dishes were left at room temperature for
solidification. Wells of diameter 6 mm were made by use of a sterile cork borer. The
test drugs were added at concentrations of 0 (control), 25, 50, 75, and 100 lg/mL.
Ciprofloxacin (30 lg/mL) and fluconazole (30 lg/mL) were used as positive
controls. The antibacterial assay plates were incubated at 37 2 °C for 24 h,
antifungal assay plates were incubated at 28 2 °C for 48 h. After the incubation
period, the diameter of the inhibition zone was measured as an indicator of the
123

Synthesis, characterization and biological evaluation
1117
activity of the compounds. Dimethyl sulfoxide was used both as a solvent and as a
negative control. No inhibition zone was observed in the control (i.e. for DMSO).
Results and discussion
Reaction of 1 with 6 equiv. 4-hydroxy-3-methoxybenzaldehyde in the presence of
K2CO3 in THF gave hexakis[(4-formyl-2-methoxy)phenoxy]cyclotriphosphazene
(2) [39]. New oxime-phosphazene hexakis[(4-(hydroxyimino)2-methoxy)phen-
oxy]cyclotriphos-phazene (3) was synthesized by reaction of 2 with hydroxylamine
hydrochloride in pyridine. Hexasubstituted compounds were obtained by reaction of
3 with allyl bromide, 4-methoxybenzoyl chloride, and methyl iodide in acetone in
the presence of K2CO3 via replacement of all the oxime protons with alkyl or acyl
groups. Hexasubstituted compounds were obtained by reaction of 3 with acetyl
chloride, propanoyl chloride, and chloroacetyl chloride in tetrahydrofuran in the
presence of triethylamine, via replacement of all the oxime protons with acyl
groups. However, reaction with 3 also furnished a trisubstituted compound (with
benzyl chloride), tetrasubstituted compounds (with 2-chlorobenzoyl chloride and
thiophene-2-carbonyl chloride) and a pentasubstituted compound (with benzoyl
chloride). Pure and defined products could not be obtained by reaction of 3 with
methacryloyl chloride and O-acetylsalicyloyl chloride. All products were generally
in high yields. The structures of the compounds were determined by elemental
analysis, and IR and 1H, 13C, and 31P NMR spectroscopy. A general presentation of
the reactions is shown in Scheme 1, and the structures of the compounds 3–13 are
shown in Scheme 2.
The characteristic stretching peaks in the IR spectra of the phosphazenes have
been assigned in the Experimental section. The P=N stretching vibrations, observed
between 1,186 and 1,193 cm-1, are characteristic of cyclophosphazenes. Compared
with 1, which appeared at 1,218 cm-1, these peaks are shifted to longer
wavelengths for 2–13. The presence of OH stretching vibration and the absence
of carbonyl bands in the IR spectra of 3 indicates the oxime compound. The absence
of the OH stretching vibration in the IR spectra of 5, 6, 8, 9, 11, and 12 indicates that
all the hydrogen atoms of the OH groups have been replaced by alkyl or acyl
substituents. However; the presence of OH stretching vibration in the IR spectra of
4, 7, 10, and 13 indicates that not all the hydrogen atoms of the OH groups could be
replaced by benzyl, benzoyl, 2-chlorobenzoyl, and thiophene-2-carbonyl substitu-
ents respectively. C=N, C=C stretching vibrations were observed between 1508 and
1643 cm-1 in the spectra of 3–13. The carbonyl bands for 2, 5, 7, 8, 9, 10, 11, and
13 were observed at 1693, 1766, 1746, 1764, 1789, 1757, 1778, and 1736 cm-1
respectively.
The NMR data of compounds 2–13 are given in Experimental section. Typical
spectra (13C and 31P NMR spectra of compound 3) are presented in Figs. 1 and 2. In
the 31P NMR spectra of 2–13, one signal was observed as singlet. Although there
are different phosphorus environments in the molecules of 4, 7, 10, and 13, the main
peak is observed as a singlet. It is understood that the phosphorus peaks are not
affected by these changes because the substituted groups are far from the
123

1118
K. Koran et al.
R
O
3
N
OH
CH
3
N
O
H
C
3
CH
3
O
O
O
O
OR
3
R
O
3
N
N
P
N
P
N
O
O
P
N
O
H
C
O
3
O
O
CH
3
O
H
C
3
N
( 10-13 )
N
OH
R
O
3
2-chlorobenzoyl chloride ( 10 )
or
R
O
2
thiophene-2-carbonyl chloride ( 13 )
R
O
1
N
OR
N
2
HO
OR
1
CH
N
3
N
OH
CH
3
N
CH3
N
O
O
O
H
C
3
H C
3
CH
3
O
O
O
CH
3
H3
C
O
O
O
CH3
O
O
O
O
OH
R
O
O
OR
2
R O
1
OH
1
N
N
O
HO
N
N
N
N
P
P
P
N
P
N
N
P
N
N
P
N
O
O
O
O
O
P
O
P
P
N
N
N
O
H3C
O
O
O
H
C
O
O
O
3
benzoylchloride
H
C
O
O
O
3
O
O
CH3
CH
3
CH
3
O
O
O
H3C
H
C
3
H C
3
N
N
N
OH
N
N
HO
OR
( 3 )
N
2
OR
1
( 7 )
R
O
( 5, 6, 8, 9, 11, 12 )
R O
1
2
benzylchloride ( 4 )
R1 : -C2H3O ( 5 ) , -C3 H5 ( 6 ) , -C3H5O ( 8 ) ,
-C8H7O2 ( 9 ) ,-C2H2OCl ( 11 ) ,-CH3
2 : -C7H5O ( 7 )
( 12 )
R
R
O
4
N
OH
R3 : -C7H4OCl ( 10 ) , C5 H3OS ( 13 )
4 : -C7H7 ( 4 )
CH
3
N
R
O
H
C
3
CH
O
O
O
3
O
OR
4
HO
N
N
P
N
P
N
O
O
P
N
O
H
C
O
3
O
O
CH
3
O
H
C
3
N
( 4 )
N
OH
R
O
4
Scheme 1 General presentation of the reactions
phosphorus atoms in these compounds. The 31P NMR shifts of 2–13 change
between 7.82 and 8.99 ppm as a singlet. These data indicate that compounds 2–13
have one isomer. However, in our similar published studies, we also observed weak
peaks arising because of the syn and anti isomerism of the –C=N groups, so we
obtained compounds that are mixtures of syn and anti isomers from the reactions of
hexakis(4-[(hydroxyimino)methyl]phenoxy)cyclotriphosphazene and hexakis(4-
[(1)-N-hydroxyethanimidoyl] phenoxy)cyclotriphosphazene with different alkyl
and acyl halogens [30, 31]. It may be that minor isomers were removed during
purification, so we observed one isomer only in this study.
1
The H and 13C NMR data also confirm the structures of 3–13 (Scheme 2).
Detailed 1H NMR spectral data are given in the Experimental section. In the
1H NMR spectra, the aldehyde proton for 2 was observed at 9.86 ppm. The
azomethine protons for 3–13 were observed between 8.02 and 8.82 ppm. In the
1H NMR spectra, OH protons were observed at 10.42, 10.40, 10.61, 10.30, and
10.60 ppm for 3, 4, 7, 10, and 13, respectively. It was apparent from the integral
intensities that there are six OH protons in 3, which is the original oxime-
123

Synthesis, characterization and biological evaluation
1119
7
7
H3CO
H3CO
9
O
3
5
3
5
2
2
O
N
H
4
4
N3P3
N3P3
O
O
1
8
1
8
6
6
6
6
2
3
13
12
7'
14
8'
3'
6'
7
H3CO
OH
3
8
2'
1'
H3CO
O
N
2
4'
10
N
4
9
11
N3P3
5'
O
5
1
O
6
3
3
4
7
7
3
3
6
8
3
8
H3CO
O
O
H CO
O
2
1
2
N
N
4
5
4
9
9
N3P3
N P
3
3
C
H3
5
10
1
O
10
O
CH
2
6
11
6
6
5
6
13
12
7'
14
8'
3'
7
H3CO
O
H
3
6
8
2'
1'
H3CO
O
N
2
1
9
4'
5'
10
N
4
5
11
N3P3
O
O
6'
O
5
7
14
OCH
3
7
8
3
6
13
12
H CO
3
O
O
2
7
CO
3
8
N
4
5
9
H
O
3
2
9
N
P
3
10
3
N
P
N
3
3
4
5
11
10
1
O
C
H
O
3
1
11
O
6
6
6
8
9
Scheme 2 The structures of compounds 2–13
123

1120
K. Koran et al.
14
15
13
12
7'
14
8'
3'
6'
H3CO
O
H
2'
7
3
6
8
N
4'
5'
H3CO
O
9
2
1
10
N
N3P3
11
Cl
4
5
1'
O
O
O
2
4
10
7
H
CO
3
7
8
3
3
5
H3CO
O
O
2
2
N
O
N
4
5
9
CH
9
4
N
P
3
N3P3
3
3
O
1
8
10
1
O
Cl
6
6
6
6
11
12
11
12
7'
14
OH
8'
3'
6'
7
H3CO
3
8
10
2'
1'
13
H3CO
O
2
1
9
N
4'
5'
N
S
4
5
N3P3
O
O
O
6
2
4
13
Scheme 2 continued
phosphazene, three OH protons in 4, one OH proton in 7, two OH protons in 10 and
two OH protons in 13. These observations for 4, 7, 10, and 13 indicate that the
benzyl, benzoyl, 2-chlorobenzoyl, and thiophene-2-carbonyl groups have not
replaced all the OH protons in 4, 7, 10, and 13 respectively.
The detailed 13C NMR spectral data are given in the Experimental section. All of
the carbon peaks in the compounds are visible in the 13C NMR spectra, as expected.
The aldehyde carbon atom was observed at 191.2 ppm for 2. The azomethine
protons for 3–13 were observed at between 150.87 and 158.91 ppm. The carbonyl
carbon for 5, 7, 8, 9, 10, 11, and 13 were observed 168.56, 167.60, 171.60, 167.31,
162.88, 168.88, and 163.19 ppm respectively.
The newly synthesized phosphazenes were screened for their in-vitro antimicro-
bial activity against two Gram-positive bacteria, S. aureus and Enterococcus
faecalis, two Gram-negative bacteria, E. coli and K. pneumoniae, and the fungal
123

Synthesis, characterization and biological evaluation
1121
140 130 120 110 100 90 80 70 60 50 40 30 20 10
0
-10 -20 -30 -40 -50 -60 -70 -80 -90 -100
-120
-140
f1 (ppm)
Fig. 1 31P NMR spectrum of compound 3
Fig. 2 13C NMR spectrum of compound 3
strains Aspergillus niger and Candida albicans, by the agar well diffusion method
using ciprofloxacin and fluconazole as control drugs for antibacterial and antifungal
activity, respectively. The pathogens were cultured on the nutrient agar medium
(antibacterial activity) and Sabouraud dextrose agar (antifungal activity). DMSO
was used as solvent and as negative control. The susceptibility was assessed on the
basis of the diameters of zones of inhibition against the organisms. Inhibition zones
were measured and compared with those for ciprofloxacin for antibacterial activity
and fluconazole for antifungal activity. The diameters of zones of inhibition,
measured in mm, for antibacterial and antifungal tests are listed in Table 1.
Minimum inhibitory concentrations (MIC) are listed in Table 2. It is apparent the
compounds had different inhibitory activity against these microorganisms. The
123

1122
K. Koran et al.
Table 1 Antibacterial and antifungal activity of the synthesized compounds (in-vitro activity zone of
inhibition in mm)
Compd. no.
Gram-positive
Gram-negative
Fungi
S. aureus
E. faecalis
E. coli
K. pneumonia
A. niger
C. albicans
2
13
25
18
21
26
27
15
24
22
20
26
28
29
–
15
24
20
21
25
30
16
23
19
18
25
29
30
–
17
23
18
20
24
28
14
22
20
21
24
27
28
–
16
26
19
19
26
29
17
26
23
22
27
30
31
–
5
7
4
8
3
4
6
10
6
5
4
6
7
11
18
3
7
16
5
8
9
6
8
10
8
6
11
5
10
12
19
–
12
8
13
18
–
Ciprofloxacin
Fluconazole
Control
20
–
23
–
–
–
–
–
– no activity
Table 2 Minimum inhibitory concentration (lg/mL)
Compd. no.
Gram-positive
Gram-negative
Fungi
S. aureus
E. faecalis
E. coli
K. pneumonia
A. niger
C. albicans
2
100
25
50
50
25
25
100
25
50
50
25
25
12.5
–
100
25
50
50
25
25
100
25
50
50
25
25
12.5
–
100
25
50
50
25
25
100
25
50
50
25
25
12.5
–
100
25
50
50
25
25
100
25
50
50
25
25
[100
[100
[100
[100
[100
75
[100
[100
[100
[100
[100
75
3
4
5
6
7
8
[100
[100
[100
[100
[100
75
[100
[100
[100
[100
[100
75
9
10
11
12
13
a
–
Ciprofloxacin
Fluconazole
12.5
–
–
12.5
12.5
a
No activity
123

Synthesis, characterization and biological evaluation
1123
efficacy of compounds 7 and 13 was almost equal to that of the standard drug
ciprofloxacin against E. faecalis and E. coli, respectively; they also had good
activity against all the other microorganisms, with inhibition zones 27–30 mm at
25 lg/mL. Compounds 3, 6, 9, and 12 had significant activity against all four
microorganisms, with inhibition zones ranging between 22 and 27 mm at 25 lg/mL.
Compounds 4, 5, 10, and 11 had moderate activity, with inhibition zones ranging
between 18 and 23 mm at 50 lg/mL. Compounds 2 and 8 had least activity, with
inhibition zones of 13–17 mm at 100 lg/mL. The in-vitro antifungal evaluation
revealed that the activity of all the compounds was relatively weak compared to
their antibacterial activity. Unexpectedly, all target compounds had negative
efficacy except compounds 7 and 13 which had moderate activity. In general, the
compounds had better antibacterial than antifungal activity.
Conclusion
For preparation of phosphazenes containing oxime groups (in high yield), this
method is very suitable. Compounds 2–13 had different antibacterial activity. The
thiophene ring in compound 13 had a positive effect on antibacterial effectiveness
which resulted in better antibacterial activity against all four bacterial pathogens
than all the other compounds except compound 7, the efficacy of which was almost
equal. Compound 2, which does not contain an oxime group, and compound 8,
which contains a propanoyloxime group, were least active (active at 100 lg/mL
only). We can make no generalizations about the antibacterial and antifungal
activity of oxime phosphazenes because the literature does not contain sufficient
basic information on the subject. We hope this original work is a potentially useful
addition to the literature and can lead to other high-polymer and biological work.
Acknowledgments The authors are grateful to the Firat University Research Fund for financial support
of this work (project no: FF.11.04).
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