Syntheses and Spectral Properties of Longwave Absorbing and Fluorescing Substrates for the Direct and Continuous Kinetic Assay of Carboxylesterases, Phosphatases, and Sulfatases

Article abstract of DOI:10.1007/BF00798280

Syntheses, absorption and fluorescence properties for a series of new enzyme substrates are described, which are derived from 7-hydroxycoumarins possessing electron-withdrawing substituents in position 3.The new substrates are advantageous over existing ones in that they exhibit longwave absorption and fluorescence maxima as well as large Stokes' shifts.In addition, their pK_a values, which are usually between 6.0 and 7.0, allow the direct and continuous kinetic assay of hydrolases such as esterases, phosphatases, and sulfatases. - Keywords: Enzyme substrates; Absorption spectra; Fluorescence spectra