Solid-phase enzymatic synthesis of a lewis a trisaccharide using an acceptor reversibly bound to Sepharose

Article abstract of DOI:10.1080/07328309708006516

The disaccharide 2-aminoethyl O-β-D-galactopyranosyl-(1→3)-2-acetamido-2-deoxy-β-D- glucopyranoside was reacted with thiobutyrolactone to give a disaccharide with a thiol group on the aglycone. This disaccharide was reacted with activated Thiopropyl Sepharose, which gave a disaccharide bound to Sepharose via a disulphide bond. Enzymatic fucosylation, using GDP-fucose and partially purified human milk fucosyltransferase, gave a trisaccharide in good yield, which was cleaved from Sepharose by treatment with mercaptoethanol or dithiothreitol.

Full text of DOI:10.1080/07328309708006516

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Journal of Carbohydrate Chemistry
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Solid-Phase Enzymatic Synthesis of a
Lewis a Trisaccharide Using an Acceptor
Reversibly Bound to Sepharose
O. Blixt a & T. Norberg a
a Department of Chemistry , Swedish University of Agricultural
Sciences , P.O. Box 7015, S-750 07, Uppsala, Sweden
Published online: 21 Aug 2006.
To cite this article: O. Blixt & T. Norberg (1997) Solid-Phase Enzymatic Synthesis of a Lewis a
Trisaccharide Using an Acceptor Reversibly Bound to Sepharose, Journal of Carbohydrate Chemistry,
16:2, 143-154, DOI: 10.1080/07328309708006516
To link to this article: http://dx.doi.org/10.1080/07328309708006516
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