Journal of the American Chemical Society p. 2395 - 2400 (1995)
Update date:2022-08-29
Topics:
Draths
Frost
A biocatalytic alternative to currently employed industrial synthesis of catechol has been developed Klebsiella pneumoniae genes encoding 3-dehydroshikimate dehydratase (aroZ) and protocatechnic acid decarboxylase (aroY) were introduced into an Escherichia coli construct that synthesizes elevated levels of 3-dehydroshikimic acid. One of the resulting biocatalysts, E. coli AB2834/pKD136/pKD9.069A, synthesizes 18.5 ± 2.0 mM catechol from 56 mM D-glucose on 1 L scale.
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