ChemBioChem
10.1002/cbic.201700415
FULL PAPER
The resin-coimmobilized enzyme particles of LDH-7αHSDH@ES-
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M. M. Zheng, K. C. Chen, R. F. Wang, H. Li, C. X. Li, J. H. Xu, J. Agric.
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103 (3.5 g, ca. 5 mL) were packed into a water-jacketed glass
[
[
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column (D:16 mm, H: 60 mm). A substrate solution containing 10
710−724.
+
mM CDCA, 30 mM sodium pyruvate, 0.5 mM NAD and 100 mM
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potassium phosphate buffer (pH 8.0) was continuously fed into
the immobilized enzyme column either from top to bottom or from
bottom to top using a peristaltic pump (BT100-1F, Baoding Longer
Precision Pump Co., Ltd., China) at a variety of flow rates (0.3–
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The coimmobilized enzyme particles of 7βHSDH-GDH@ES-103
(
3.5 g, 10 g or 17.5 g) were packed into another water-jacketed
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were connected in series by a rubber hose. A substrate solution,
4
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+
containing 10 mM CDCA, 0.5 mM NAD , 0.5 mM NADP , 30 mM
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two enzyme columns from top to bottom at a variety of flow rates
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-1
(
0.3–1.5 mL min ) using the aforementioned peristaltic pump.
The reaction temperature was maintained at 30°C by circulating
a thermostat water in the jackets surrounding the enzyme
columns.
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1
Keywords: Cascade reaction • Coimmobilized enzymes •
Continuous operation • Packed bed bioreactor • Ursodeoxycholic
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