Isolation and characterization of 5-carbamoylmethyluridine and 5- carbamoylmethyl-2-thiouridine from human urine

Article abstract of DOI:10.1080/07328319908044873

Two new modified uracil nucleosides, 5-carbamoylmethyuridine (ncm⁵U, I) and 5-carbamoylmethyl-2-thiouridine (ncm⁵s²U, II) were isolated from a 24 hr collection of a normal human urine. The structures were assigned on the basis of UV, NMR and mass spectral data and confirmed by comparison of the spectral data and HPLC mobilities with those of authentic samples. On the basis of experimental data it appears possible that 5-carbamoylmethyl- 2-thio-uridine (ncm⁵s²U, II) may be a degradation product produced from a labile precursor by the chemical treatments during the isolation procedure. However, the other nucleoside (ncm⁵U,I) certainly appears to be of metabolic origin and was also found in the urines of one chronic myelogenous leukemia and one lung carcinoma patient.

Full text of DOI:10.1080/07328319908044873

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Isolation and Characterization of
5-Carbamoylmethyluridine and 5-
Carbamoylmethyl-2-Thiouridine from
Human Urine
G. B. Chheda a , H. B. Patrzyc a , H. A. Tworek a & S. P. Dutta a
a Department of Biophysics, Roswell Park Cancer Institute, Buffalo,
NY, 14263
Published online: 04 Oct 2006.
To cite this article: G. B. Chheda , H. B. Patrzyc , H. A. Tworek & S. P. Dutta (1999) Isolation and
Characterization of 5-Carbamoylmethyluridine and 5-Carbamoylmethyl-2-Thiouridine from Human
Urine, Nucleosides and Nucleotides, 18:10, 2155-2173, DOI: 10.1080/07328319908044873
To link to this article: http://dx.doi.org/10.1080/07328319908044873
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