Synthesis of an Aspartame Precursor by Immobilized Thermolysin in an Organic Solvent

Article abstract of DOI:10.1021/jo00338a045

The synthesis of N-(benzyloxycarbonyl)-L-aspartyl-L-phenylalanine methyl ester, the precursor of the synthetic sweetener aspartame, from N-(benzyloxycarbonyl)-L-aspartic acid an L-phenylalanine methyl ester was carried out in an apparent single phase of the organic solvent by using thermolysin immobilized with various methods.