SYNTHESIS OF TRITIUM-LABELED COMPONENTS OF NUCLEIC ACIDS OF THE HYPOXANTHINE SERIES

Article abstract of DOI:10.1007/BF00602471

The synthesis of <8-3H> and <2,8-3H>hypoxanthine, inosine, inosine 5'-mono-, -di- and -triphosphates, and also of 2'-deoxy<2,2'-8-3H>inosine from the corresponding labeled compounds of the adenine series with the aid of the de-amination reaction is described.De-amination was carried out with sodium nitrite in the presence of acetic acid.In the case of bases and nucleosides, the separation of the reaction mixtures with simultaneous desalting of the final products was achieved by column chromatography on Sephadex G-10 or SE C-25 with elution by water.For nucleotides, the isolation process included chromatography on DEAE-Sephadex A-25 (HCOO^- or Cl^-) and Dowex 1 * 8 (Cl^-) followed by desalting with the aid of reprecipitation or adsorption on Carboraffin activated carbon.The molar radioactivities of the compounds synthesized amounted to 370-2220 TBq/mole (10-60 kCi/mole) and corresponded to the molar radioactivities of the initial compounds of the adenine series.