Laser-Induced Room-Temperature Phosphorescence Detection of Bnzopyrene-DNA Adducts

Article abstract of DOI:10.1021/ac00135a006

The room-temperature phosphorescence (RTP) spectrum of benzopyrene-r-7,t-8,9,10-tetrahydrotetrol (BP-tetrol) has been measured using laser excitation.The BP-terol was obtained by acid hydrolysis of the r-7,t-8-dihydroxy-t-9,10-epoxy-7,8,9,10-tetrahydrobenzopyrene (BPDE)-DNA adducts.BPDE is the ultimate carcinogenic metabolite of benzopyrene (BP).The BP-tetrol sample was measured on a filter paper substrate pretreated with a heavy-atom salt, thallium acetate, used to increase the phosphorescence signal of BP-tetrol.The detection limit of BPDE in vitro modified BPDE-DNA was about 15 fmol.The results indicate the RTP would be useful as a simple and practical screening tool for monitoring BPDE-DNA adducts and related BP metabolites in biological samples.