Synthesis of oligonucleotide derivatives with pyrene group at sugar fragment

Article abstract of DOI:10.1016/0040-4039(91)80166-4

The synthesis of oligonucleotide derivatives possessing pyrene group at a specific sugar residue has been described. The incorporation of the pyrene was accomplished by preparation of the pyrene-modified uridine, 2'-(1-pyrenylmethyl)uridine, which was then converted to the protected phosphorobisdiethylamidite. This reagent was used for the solid-phase synthesis of oligonucleotide-pyrene conjugates. The purification of the conjugates was effected by reversed-phase HPLC. The oligonucleotide-pyrene conjugates synthesized here can bind to their complementary sequences with a cooperative interaction between the pyrene group and the adjacent base-pairs.