Specificity of KLK3 subsites S3, S2, S01, and S20 on the hydrolysis of FRET peptide series Abz-KXRSSKQ-EDDnp

July 26, 2024

• Title:       

  Specificity of KLK3 subsites S₃, S₂, S⁰₁, and S₂⁰ on the hydrolysis of FRET peptide series Abz-KXRSSKQ-EDDnp

• Image Source:

  Substrate specificity and inhibition of human kallikrein-related peptidase 3 (KLK3 or PSA) activated with sodium citrate and glycosaminoglycans

• Mark:

  Fig. 9

• Associated context:

  We further explored the arginyl-hydrolase activity of KLK3 by assaying four series of FRET peptides derived from Abz-KLR; SSKQ-EDDnp, where R at the P1 position was fixed and K(P3),  00L(P2), S(P1 ) and S(P2 ) were substituted by natural amino acids.  Fig. 9 presents the initial velocities for hydrolysis of all the series under the same conditions as those used for the analysis of S1 subsite specificity in the presence of 1.5 M sodium citrate.