Steroids p. 21 - 37 (1980)
Update date:2022-08-12
Topics:
Satyaswaroop, P. G.
Frost, A.
Gurpide, E.
Human endometrial adenocarcinoma cells (HEC-1 line) were incubated with 14C-progesterone.Four major labeled metabolites, 3β-hydroxy 5α-pregnan-20-one, 5α-pregnane-3β,20α-diol, 20α-hydroxy-4-pregnen-3-one and 5α-pregnane-3,20-dione were separated by thin layer chromatography, further purified by high pressure liquid chromatography, and finally identified by addition of carriers and crystallization to constant specific activity.Among these metabolites, 5α-pregnane-3β,20α-diol seems characteristic of this cell line since its formation from labeled progesterone was not detected in normal endometrium or in 2 specimens of endometrial adenocarcinoma.The growth of HEC cells was unaffected by either progesterone or medroxyprogesterone acetate, a slowly metabolized progestin, at about 10-6 M levels but was inhibited by about 10-5 M concentrations of these compounds.
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