90-80-2Relevant articles and documents
carba Nicotinamide Adenine Dinucleotide Phosphate: Robust Cofactor for Redox Biocatalysis
D?ring, Manuel,Sieber, Volker,Simon, Robert C.,Tafertshofer, Georg,Zachos, Ioannis
supporting information, p. 14701 - 14706 (2021/05/13)
Here we report a new robust nicotinamide dinucleotide phosphate cofactor analog (carba-NADP+) and its acceptance by many enzymes in the class of oxidoreductases. Replacing one ribose oxygen with a methylene group of the natural NADP+ was found to enhance stability dramatically. Decomposition experiments at moderate and high temperatures with the cofactors showed a drastic increase in half-life time at elevated temperatures since it significantly disfavors hydrolysis of the pyridinium-N?glycoside bond. Overall, more than 27 different oxidoreductases were successfully tested, and a thorough analytical characterization and comparison is given. The cofactor carba-NADP+ opens up the field of redox-biocatalysis under harsh conditions.
Efficient improvement in non-enzymatic glucose detection induced by the hollow prism-like NiCo2S4electrocatalyst
Chen, Qiwen,Chen, Xiaojun,Chu, Dandan,Chu, Xue-Qiang,Ge, Danhua,Yan, Li
, p. 15162 - 15169 (2021/11/17)
Hollow prism-like NiCo2S4 materials (NiCo2S4 HNPs) were successfully fabricated by a two-step method. Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and powder X-ray diffraction (XRD) confirmed the morphology and structure of the as-prepared NiCo2S4 nanoprisms. A non-enzymatic sensor based on NiCo2S4 HNPs was constructed with outstanding electrochemical activity towards glucose oxidation in alkaline medium. The sensor showed a rapid response time (～0.1 s), a high sensitivity of 82.9 μA mM-1 cm-2, a wide linear range (0.005-20.2 mM) and a detection limit of 0.8 μM (S/N = 3) with a good selectivity and reproducibility. Additionally, the proposed electrode also confirmed the feasibility in practical blood serum. These results indicate that NiCo2S4/ITO has great potential in the development of non-enzymatic glucose sensor applications.
Anticancer and antileishmanial in vitro activity of gold(I) complexes with 1,3,4-oxadiazole-2(3H)-thione ligands derived from δ-D-gluconolactone
Espinosa, Andrés Villase?or,Costa, Danilo de Souza,Tunes, Luiza Guimar?es,Monte-Neto, Rubens L. do,Grazul, Richard Michael,de Almeida, Mauro Vieira,Silva, Heveline
, p. 41 - 50 (2020/07/28)
Four gold(I) complexes conceived as anticancer agents were synthesized by reacting [Au(PEt3)Cl] and [Au(PPh3)Cl] with ligands derived from δ-d-gluconolactone. The ligands’ structure was designed to combine desired biological properti
The non-enzymatic electrochemical detection of glucose and ammonia using ternary biopolymer based-nanocomposites
Bano, Sayfa,Ganie, Adil Shafi,Sultana, Saima,Khan, Mohammad Zain,Sabir, Suhail
, p. 8008 - 8021 (2021/05/21)
The non-enzymatic electrochemical detection of glucose and aqueous ammonia was carried out using ternary bio-nanocomposites. A co-precipitation method was employed to synthesize cadmium stannate nanoparticles, while bio-nanocomposites of polyaniline-cadmium stannate-chitosan were synthesized via the chemical oxidative polymerization technique. Functional group analysis, structural and morphological studies were further carried out via Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and scanning and transmission electron microscopy techniques. The electrode kinetics and electrochemical properties were validated via cyclic voltammetry, electrochemical impedance spectroscopy (EIS), and chronoamperometric techniques. The sensing properties of the electrocatalysts were explored using cyclic voltammetry and amperometry for the detection of glucose and ammonia solution of varying concentrations using NaOH electrolyte. The electrochemical activity of the electrocatalysts were optimized using different weight percentages of chitosan, and bio-nanocomposites of polyaniline/cadmium stannate/chitosan (10%) showed excellent electrocatalytic and electrochemical activities. Moreover, the bio-nanocomposites have a low detection limit and short response time toward different concentrations of glucose and ammonia solution. Apart from these studies, the sensors have also been evaluated for an anti-interference study. The developed novel sensors present significant potential to be efficiently utilized on a large scale, as they also show effective stability and reproducibility, along with a high level of sensitivity for the given target analytes. This journal is
Synthesis of Cu(OH)F microspheres using atmospheric dielectric barrier discharge microplasma: a high-performance non-enzymatic electrochemical sensor
Hu, Zhangmei,Huang, Ke,Jiang, Xue,Wang, Qiang,Yu, Huimin,Zhao, Li,Zhou, Jiaxin
supporting information, p. 18277 - 18281 (2021/10/19)
In this study, Cu(OH)F microspheres suppported on a carbon cloth (Cu(OH)F MS/CC) were rapidly synthesized (at 90 V with 20 min) using an atmospheric dielectric barrier discharge microplasma (DBD). As a multifunctional electrochemical sensor for the detection of glucose (Glu), formaldehyde and hydrogen peroxide, it can accurately detect blood glucose levels in actual serum samples and can determine the contents of formaldehyde and hydrogen peroxide in water samples. Furthermore, it shows good sensitivity and selectivity, which confirmed the feasibility of the Cu(OH)F microsphere electrode for electrochemical sensing. This method was not only rapid and mild (at room temperature and atmospheric pressure) but also provided a promising route for the preparation of nanomaterials for electrochemical sensors.
Enzyme Kinetics via Open Circuit Potentiometry
Smith, Lettie A.,Glasscott, Matthew W.,Vannoy, Kathryn J.,Dick, Jeffrey E.
, p. 2266 - 2273 (2020/02/04)
We demonstrate the application of open circuit potentiometry (OCP) to measure enzyme turnover kinetics, kturn. The electrode surface will become poised by the addition of a well-behaved redox pair, such as ferrocenemethanol/ferrocenium methanol (FcMeOH/FcMeOH+), which acts as the cosubstrate for the enzymatic process. A measurable change in potential results when an enzyme consumes the one-electron transfer mediator. Glucose oxidase was studied as a test-case, but the method is generalizable across oxidoreductase enzymes that rely on electron transfer mediators. In the presence of glucose and FcMeOH+, glucose oxidase delivers electrons to FcMeOH+, and the potential changes with respect to the Nernst equation. A theoretical model incorporating enzymatic rate expressions into the Nernst equation was derived to explain the observed potential transients, and experimental data fit theory well. A similar experiment was performed using amperometry on ultramicroelectrodes (UMEs). Here, the same enzymatic rate expression may be incorporated into the equation for steady-state flux to an UME to obtain kturn. While similar kinetic information was obtained from the potentiometric and amperometric responses, potentiometry is independent of electrode size and mass transfer effects. Finally, we show how kturn changes as a function of one-electron mediator. Our results may eventually find applications to biosensors, where electrode fouling plagues long-term sensor performance.
Engineering the valence state of ZIF-67 by Cu2O for efficient nonenzymatic glucose detection
Guo, Kailu,Xu, Cailing,Yang, Nian,Zhang, Yanwen
, p. 2856 - 2861 (2020/04/17)
The valence state regulation of Co-based electrocatalysts is extremely important and greatly challenging to enhance the electrochemical performance toward glucose oxidation. Herein, Cu2O?ZIF-67 composites with fine-tuned valence states were rationally constructed for boosting glucose oxidation. X-ray photoelectron spectroscopy (XPS) and cyclic voltammetry (CV) analysis confirm that the content of the high valence state Co (Co3+) in Cu2O?ZIF-67 is much higher than that in the individual ZIF-67 due to the synergistic effects between ZIF-67 and Cu2O. As a result, Co3+-rich Cu2O?ZIF-67 composite exhibits remarkable activity toward glucose electro-oxidation with two linear response ranges of glucose concentration, from 0.01 to 10 mM and 10 to 16.3 mM, high sensitivities of the linear ranges (307.02 and 181.34 μA mM-1 cm-2) as well as a low detection limit (6.5 μM). This research provides a novel avenue for the progress of highly efficient electrocatalysts for nonenzymatic glucose oxidation.
Protein Spherical Nucleic Acids for Live-Cell Chemical Analysis
Samanta, Devleena,Ebrahimi, Sasha B.,Kusmierz, Caroline D.,Cheng, Ho Fung,Mirkin, Chad A.
, p. 13350 - 13355 (2020/09/09)
We report the development of a new strategy for the chemical analysis of live cells based on protein spherical nucleic acids (ProSNAs). The ProSNA architecture enables analyte detection via the highly programmable nucleic acid shell or a functional protein core. As a proof-of-concept, we use an i-motif as the nucleic acid recognition element to probe pH in living cells. By interfacing the i-motif with a forced-intercalation readout, we introduce a quencher-free approach that is resistant to false-positive signals, overcoming limitations associated with conventional fluorophore/quencher-based gold NanoFlares. Using glucose oxidase as a functional protein core, we show activity-based, amplified sensing of glucose. This enzymatic system affords greater than 100-fold fluorescence turn on in buffer, is selective for glucose in the presence of close analogs (i.e., glucose-6-phosphate), and can detect glucose above a threshold concentration of ～5 μM, which enables the study of relative changes in intracellular glucose concentrations.
Cobalt sulfides/carbon nanohybrids: A novel biocatalyst for nonenzymatic glucose biofuel cells and biosensors
Li, Zihan,Li, Gangyong,Wu, Zhongdong,Jiao, Shuqiang,Hu, Zongqian
, p. 32898 - 32905 (2019/10/23)
Exploring high-performance electrocatalysts is of great importance in developing nonenzymatic biofuel cells. Hybrid nanostructures with transition metal compounds and carbon nanomaterials exhibit excellent electrocatalytic activity and have emerged as promising low-cost alternatives for various electrochemical reactions. Herein, we report cobalt sulfide/carbon nanohybrids via a facile synthesis, which have excellent electrocatalytic activity for glucose oxidation and oxygen reduction reaction. The nonenzymatic glucose biofuel cells equipped with cobalt sulfide/carbon nanohybrids deliver a high open circuit voltage of 0.72 V with a maximum open power density of 88 μW cm-2, indicating that cobalt sulfide/carbon nanohybrids are high performance biocatalysts for bioenergy conversion.
Nickel oxide decorated MoS2 nanosheet-based non-enzymatic sensor for the selective detection of glucose
Jeevanandham, Gayathri,Jerome,Murugan,Preethika,Vediappan, Kumaran,Sundramoorthy, Ashok K.
, p. 643 - 654 (2020/01/23)
Understanding blood glucose levels in our body can be a key part in identifying and diagnosing prediabetes. Herein, nickel oxide (NiO) decorated molybdenum disulfide (MoS2) nanosheets have been synthesized via a hydrothermal process to develop a non-enzymatic sensor for the detection of glucose. The surface morphology of the NiO/MoS2 nanocomposite was comprehensively investigated by field-emission scanning electron microscopy (FE-SEM), high-resolution transmission electron microscopy (HR-TEM), powder X-ray diffraction (PXRD), X-ray photoelectron spectroscopy (XPS) and Brunauer-Emmett-Teller (BET) analysis. The electro-catalytic activity of the as-prepared NiO/MoS2 nanocomposite towards glucose oxidation was investigated by cyclic voltammetry, electrochemical impedance spectroscopy (EIS) and amperometry in 0.1 M NaOH. The NiO/MoS2 nanocomposite-based sensor showed outstanding electrocatalytic activity for the direct electro-oxidation of glucose due to it having more catalytic active sites, good conductivity, excellent electron transport and high specific surface area. Meanwhile, the NiO/MoS2 modified glassy carbon electrode (GCE) showed a linear range of glucose detection from 0.01 to 10 mM by amperometry at 0.55 V. The effect of other common interferent molecules on the electrode response was also tested using alanine, l-cysteine, fructose, hydrogen peroxide, lactose, uric acid, dopamine and ascorbic acid. These molecules did not interfere in the detection of glucose. Moreover, this NiO/MoS2/GCE sensor offered rapid response (2 s) and a wide linear range with a detection limit of 1.62 μM for glucose. The reproducibility, repeatability and stability of the sensor were also evaluated. The real application of the sensor was tested in a blood serum sample in the absence and presence of spiked glucose and its recovery values (96.1 to 99.8%) indicated that this method can be successfully applied to detect glucose in real samples.
