101-98-4Relevant articles and documents
A Universal Labeling Strategy for Nucleic Acids in Expansion Microscopy
Hofkens, Johan,Leen, Volker,Rohand, Taoufik,Vandereyken, Katy,Vanheusden, Marisa,Voet, Thierry,Wen, Gang
supporting information, p. 13782 - 13789 (2021/09/11)
Expansion microscopy (ExM) enables the nanoscale imaging of ribonucleic acids (RNAs) on a conventional fluorescence microscope, providing information on the intricate patterns of gene expression at (sub)cellular resolution and within spatial context. To extend the use of such strategies, we examined a series of multivalent reagents that allow the labeling and grafting of deoxyribonucleic acid (DNA) oligonucleotide probes in a unified approach. We show that the reagents are directly compatible with third-generation in situ hybridization chain reaction RNA FISH (fluorescence in situ hybridization) techniques while displaying complete retention of the targeted transcripts. Furthermore, we validate and demonstrate that our labeling method is compatible with multicolor staining. Through oligonucleotide-conjugated antibodies, we demonstrate excellent performance in ×4 ExM and ×10 ExM, achieving a resolution of ~50 nm in ×10 ExM for both pre- and postexpansion labeling strategies. Our results indicate that our multivalent molecules enable the rapid functionalization of DNA oligonucleotides for ExM.
Biquaternary ammonium compound and preparation method and application thereof
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Paragraph 0142-0145, (2021/06/02)
The invention provides a biquaternary ammonium compound and a preparation method and application thereof. The biquaternary ammonium compound is shown as a formula (I). The compound disclosed by the invention has quick-acting and ultra-short-acting non-depolarized muscle relaxation molecular activity, is quick in acting and can generate a complete muscle relaxation effect under the condition of low administration dosage, and the time required for complete recovery of muscle strength is short and is obviously shorter than that of a positive drug cisatracurium and a depolarized muscle relaxation drug succinylcholine; the problems of non-depolarized muscle relaxant in the prior art are effectively solved, and the compound can be used for preparing the non-depolarized muscle relaxant with an excellent effect and has a good prospect.
DEUTERATED 3-(4,5-SUBSTITUTED PYRIMIDINAMINE) PHENYL DERIVATIVES AND APPLICATIONS THEREOF
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Paragraph 0025-0028; 0079-0082, (2019/06/27)
The present invention discloses a type of deuterated 3-(4,5-substituted aminopyrimidine)phenyl derivatives and use thereof. The derivatives are compounds of Formula (I) or pharmaceutically acceptable salts thereof. These compounds or salts thereof can be used for treatment or prevention of diseases or illnesses through certain mutant forms of epidermal growth factor receptors, inhibit the growth of various tumor cells effectively, and inhibit other proteases of EGFR and Her families, they can be used for preparing anti-tumor drugs.