696-60-6Relevant articles and documents
Method for synthesizing hydroxybenzylamine
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Paragraph 0046-0052, (2020/02/27)
The invention discloses a method for synthesizing hydroxybenzylamine, and belongs to the technical field of organic synthesis. The principle of the method comprises that a demethylation reaction is carried out on methoxybenzylamine under the action of hydrobromic acid; the method is characterized in that methoxybenzylamine and hydrobromic acid are distilled in a reflux state to remove redundant water so as to increase the reaction temperature and increase the concentration of the hydrobromic acid in a reaction mixture, so that the demethylation effect of hydrobromic acid on methoxybenzylamineis enhanced, the reaction time is shortened, and the conversion rate is increased; when no bromomethane gas generation is observed, distillation is continued, excessive hydrobromic acid is recovered to further improve the reaction temperature and the conversion rate, meanwhile, the consumption of the raw material hydrobromic acid is reduced, and the treatment capacity of subsequent steps and the consumption of the raw material sodium hydroxide can also be reduced; therefore, the method has the advantages of simple technological process; the reaction time is short; the product is easy to purify; raw material consumption is low; the reaction yield is high.
Metagenomic discovery of a novel transaminase for valorization of monoaromatic compounds
Pawar, Sandip V.,Hallam, Steven J.,Yadav, Vikramaditya G.
, p. 22490 - 22497 (2018/06/29)
The profitability of next-generation biorefineries is acutely contingent on the discovery and utilization of biocatalysts that can valorize lignin. To this end, the metabolic catalogues of diverse microbiota have been mined previously using functional metagenomics in order to identify biocatalysts that can selectively degrade lignin into monoaromatic compounds. Herein, we have further improved the valorization factor of biorefining by deploying functional metagenomics toward the identification of a novel transaminase that can selectively functionalize lignin-derived monoaromatics to produce value-added feedstocks for pharmaceutical synthesis. We implemented a high-throughput colorimetric assay using o-xylylenediamine as the amino donor and successfully identified a transaminase that utilizes the canonical cofactor, pyridoxal 5′-phosphate, to aminate as many as 14 monoaromatic aldehydes and ketones. We subsequently identified the optimal conditions for enzyme activity towards the most favoured amino acceptor, benzaldehyde, including temperature, pH and choice of co-solvent. We also evaluated the specificity of the enzyme towards a variety of amino donors, as well as the optimal concentration of the most favoured amino donor. Significantly, the novel enzyme is markedly smaller than typical transaminases, and it is stably expressed in E. coli without any modifications to its amino acid sequence. Finally, we developed and implemented a computational methodology to assess the activity of the novel transaminase. The methodology is generalizable for assessing any transaminase and facilitates in silico screening of enzyme-substrate combinations in order to develop efficient biocatalytic routes to value-added amines. The computational pipeline is an ideal complement to metagenomics and opens new possibilities for biocatalyst discovery.
MANUFACTURING METHOD OF AROMATIC COMPOUND AND FURAN DERIVATIVE HAVING METHYLAMINO GROUP
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Paragraph 0034; 0035, (2017/10/26)
PROBLEM TO BE SOLVED: To provide a method for manufacturing an aromatic compound or a furan derivative where only aldehyde group is converted to an aminomethyl group while maintaining a structure of aromatic or furan ring from an aromatic compound or a furan derivative having an aldehyde group, capable of being conducted in a water solvent containing no organic solvent and relatively low in by-product. SOLUTION: Amine or ammonia is added in water at first to convert to imine, then a reaction is conducted by using compressive hydrogen with a pressure of 0.1 MPa to 4 MPa in the presence of a metal carried solid catalyst carrying one or more kind of metal selected from rhodium, palladium and platinum or an alloy containing these metal elements. SELECTED DRAWING: Figure 2 COPYRIGHT: (C)2017,JPOandINPIT
