1609-47-8 Usage
Chemical Properties
liquid
Uses
Different sources of media describe the Uses of 1609-47-8 differently. You can refer to the following data:
1. Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.1
2. Gentle esterifying agent. Preservative for wines, soft drinks, fruit juices.
3. Diethyl pyrocarbonate may be used in the following studies:For the modification of histidyl residues in proteins.As a nuclease inhibitor, for the extraction of undegraded nucleic acids from etiolated and green plant tissues.For the modification of linear and supercoiled plasmid DNAs.As a chemical probe to investigate the secondary structure in negatively supercoiled DNA.For cabethoxylation of histidine residues of actin.
Definition
ChEBI: The diethyl ester of dicarbonic acid.
General Description
Diethyl pyrocarbonate (DEP) is a bactericidal agent and decomposes in water to CO2 and ethanol. DEP inhibits enzymes such as DNase and RNase without affecting nucleic acids. It is useful for the preparation of mRNA from tissues.
Biochem/physiol Actions
Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.
Safety Profile
Poison by ingestion,
inhalation, and intraperitoneal routes.
Concentrated DEPC is irritating to eyes,
mucous membranes, and sh. When heated
to decomposition it emits acrid smoke and
fumes. See also ESTERS.
Purification Methods
Dissolve the ester in Et2O, wash it with dilute HCl, H2O, dry over Na2SO4, filter, evaporate and distil the residue first in vacuo then at atmospheric pressure. It is soluble in alcohols, esters, ketones and hydrocarbon solvents. A 50% w/w solution is usually prepared for general use. Treat with great CAUTION as DEP irritates the eyes, mucous membranes and skin. [Boehm & Mehta Chem Ber 71 1797 1938, Thoma & Rinke Justus Liebigs Ann Chem 624 30 1959, Beilstein 3 IV 18.]
Check Digit Verification of cas no
The CAS Registry Mumber 1609-47-8 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,6,0 and 9 respectively; the second part has 2 digits, 4 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 1609-47:
(6*1)+(5*6)+(4*0)+(3*9)+(2*4)+(1*7)=78
78 % 10 = 8
So 1609-47-8 is a valid CAS Registry Number.
InChI:InChI=1/C6H10O5/c1-3-9-5(7)6(8)11-10-4-2/h3-4H2,1-2H3
1609-47-8Relevant articles and documents
Method for making mixed high purity (meth)acrylic anhydrides
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Page column 6-7, (2008/06/13)
The invention concerns a method consisting in making a mixed (meth)acrylic anhydride of formula (I) by reacting an alkaline (meth)acrylate of formula (II) and a chloroformate of formula (III), carrying out said reaction in an aqueous medium and in the absence of amines, the mol ratio chloroformate (III)/alkaline (meth)acrylate (II) being at least equal to 1.15. R1represents H or CH3; R2represents an alkyl, alkenyl, aryl, alkaryl or aralkyl residue; and M is an alkaline metal.
Method for preparing di(organo) esters of pyrocarbonic acid
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, (2008/06/13)
Di(organo) esters of pyrocarbonic acid, e.g., dialkyl pyrocarbonates, such as diethyl pyrocarbonate, are prepared by reaction of the corresponding organohaloformate with aqueous alkali metal hydroxide, e.g., sodium hydroxide, in the substantial absence of an organic solvent and in the presence of a catalytic amount of a bis[poly(oxy(C2 -C4)alkylene)] C6 -C20 aliphatic amine, e.g., coco bis(polyoxyethylene) amine.
