6168-72-5Relevant articles and documents
Samyn et al.
, p. 2849,2858 (1976)
Selective amination of 1,2-propanediol over Co/La3O4 catalyst prepared by liquid-phase reduction
Yue, Chuan-Jun,Di, Kai,Gu, Li-Ping,Zhang, Zhen-Wei,Ding, Lin-Lin
, (2019/08/20)
The catalytic coupling of alcohol and ammonia is an environmentally friendly process. Cobalt-based catalysts, modified by supports (including CeO2, Fe3O4, Nb2O5, La3O4 and Al2O3), and prepared by the liquid-phase reduction, were used for the amination of 1,2-propanediol. The screened nano-Co/La3O4 catalyst exhibited an excellent catalytic performance of 68% conversion and 89% selectivity toward 2-amino-1-propanol under optimal conditions. The characterizations of the catalyst was performed by XRD, XPS, BET, TEM, TG, and CO2-TPD, revealing a relatively large specific surface area, strongly alkaline sites and a Co-La-O transition phase, which were responsible for the selective catalysis of 1,2-propanediol. The efficient construction of cobalt-based catalysts on the basis of the active species is key to improving the efficiency of the reaction process.
Rapid and Quantitative Profiling of Substrate Specificity of ω-Transaminases for Ketones
Han, Sang-Woo,Shin, Jong-Shik
, p. 3287 - 3295 (2019/06/21)
ω-Transaminases (ω-TAs) have gained growing attention owing to their capability for asymmetric synthesis of chiral amines from ketones. Reliable high-throughput activity assay of ω-TAs is essential in carrying out extensive substrate profiling and establishing a robust screening platform. Here we report spectrophotometric and colorimetric methods enabling rapid quantitation of ω-TA activities toward ketones in a 96-well microplate format. The assay methods employ benzylamine, a reactive amino donor for ω-TAs, as a cosubstrate and exploit aldehyde dehydrogenase (ALDH) as a reporter enzyme, leading to formation of benzaldehyde detectable by ALDH owing to concomitant NADH generation. Spectrophotometric substrate profiling of two wild-type ω-TAs of opposite stereoselectivity was carried out at 340 nm with 22 ketones, revealing subtle differences in substrate specificities that were consistent with docking simulation results obtained with cognate amines. Colorimetric readout for naked eye detection of the ω-TA activity was also demonstrated by supplementing the assay mixture with color-developing reagents whose color reaction could be quantified at 580 nm. The colorimetric assay was applied to substrate profiling of an engineered ω-TA for 24 ketones, leading to rapid identification of reactive ketones. The ALDH-based assay is expected to be promising for high-throughput screening of enzyme collections and mutant libraries to fish out the best ω-TA candidate as well as to tailor enzyme properties for efficient amination of a target ketone.